In Vivo and in Vitro Antiviral Effects of Berberine on Influenza Virus

Objective:To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods:In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells.In vivo anti-influenza virus assays were per...

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Published inChinese journal of integrative medicine Vol. 17; no. 6; pp. 444 - 452
Main Author 吴莹 李季倩 金叶智 吴珺 王谦 郝鈺
Format Journal Article
LanguageEnglish
Published Heidelberg Chinese Association of Traditional and Western Medicine 01.06.2011
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ISSN1672-0415
1993-0402
1993-0402
DOI10.1007/s11655-011-0640-3

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Abstract Objective:To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods:In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells.In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice.The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality.On days 2,4,and 6,the viral titers in the lungs were determined by hemagglutination assay;hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha(TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay;the concentrations of nitric oxide(NO) and inducible nitric oxide synthetase(iNOS) were detected by colorimetric method;reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-αand MCP-1.Results: Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus,with the therapeutic index 9.69.In vivo,berberine decreased mice mortality from 90%to 55%,reduced virus titers in the lungs on day 2 postinfection(P0.05).The lung histology scores were 1.50±0.67,4.50±1.00,and 5.50±1.00 in the berberine group on days 2,4,and 6,respectively,which were significantly reduced compared to 2.17±0.22,6.83±0.44,and 8.50±0.33 in the infected group(P0.05).The productions of NO and iNOS were repressed by berberine compared with those in the infected group(P0.01).The transcription and expression of TNF-αwere inhibited by berberine on day 4(P0.01) and day 6(P0.05),and those of MCP-1 were inhibited on day 6(P0.01) compared with the infected group.Conclusions:Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo.The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection,as well as improving the pathogenic changes by repressing inflammatory substances release
AbstractList Objective:To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods:In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells.In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice.The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality.On days 2,4,and 6,the viral titers in the lungs were determined by hemagglutination assay;hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha(TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay;the concentrations of nitric oxide(NO) and inducible nitric oxide synthetase(iNOS) were detected by colorimetric method;reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-αand MCP-1.Results: Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus,with the therapeutic index 9.69.In vivo,berberine decreased mice mortality from 90%to 55%,reduced virus titers in the lungs on day 2 postinfection(P0.05).The lung histology scores were 1.50±0.67,4.50±1.00,and 5.50±1.00 in the berberine group on days 2,4,and 6,respectively,which were significantly reduced compared to 2.17±0.22,6.83±0.44,and 8.50±0.33 in the infected group(P0.05).The productions of NO and iNOS were repressed by berberine compared with those in the infected group(P0.01).The transcription and expression of TNF-αwere inhibited by berberine on day 4(P0.01) and day 6(P0.05),and those of MCP-1 were inhibited on day 6(P0.01) compared with the infected group.Conclusions:Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo.The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection,as well as improving the pathogenic changes by repressing inflammatory substances release
Objective To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo . Methods In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2, 4, and 6, the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1. Results Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus, with the therapeutic index 9.69. In vivo , berberine decreased mice mortality from 90% to 55%, reduced virus titers in the lungs on day 2 postinfection ( P <0.05). The lung histology scores were 1.50±0.67, 4.50±1.00, and 5.50±1.00 in the berberine group on days 2, 4, and 6, respectively, which were significantly reduced compared to 2.17±0.22, 6.83±0.44, and 8.50±0.33 in the infected group ( P <0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group ( P <0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 ( P <0.01) and day 6 ( P <0.05), and those of MCP-1 were inhibited on day 6 ( P <0.01) compared with the infected group. Conclusions Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo . The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection, as well as improving the pathogenic changes by repressing inflammatory substances release.
To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.OBJECTIVETo explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2, 4, and 6, the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1.METHODSIn vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2, 4, and 6, the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1.Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus, with the therapeutic index 9.69. In vivo, berberine decreased mice mortality from 90% to 55%, reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50 ± 0.67, 4.50 ± 1.00, and 5.50 ± 1.00 in the berberine group on days 2, 4, and 6, respectively, which were significantly reduced compared to 2.17 ± 0.22, 6.83 ± 0.44, and 8.50 ± 0.33 in the infected group (P<0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group (P<0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 (P<0.01) and day 6 (P<0.05), and those of MCP-1 were inhibited on day 6 (P<0.01) compared with the infected group.RESULTSBerberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus, with the therapeutic index 9.69. In vivo, berberine decreased mice mortality from 90% to 55%, reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50 ± 0.67, 4.50 ± 1.00, and 5.50 ± 1.00 in the berberine group on days 2, 4, and 6, respectively, which were significantly reduced compared to 2.17 ± 0.22, 6.83 ± 0.44, and 8.50 ± 0.33 in the infected group (P<0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group (P<0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 (P<0.01) and day 6 (P<0.05), and those of MCP-1 were inhibited on day 6 (P<0.01) compared with the infected group.Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo. The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection, as well as improving the pathogenic changes by repressing inflammatory substances release.CONCLUSIONSBerberine exhibited antiviral effects on the influenza virus both in vitro and in vivo. The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection, as well as improving the pathogenic changes by repressing inflammatory substances release.
To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo. In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2, 4, and 6, the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1. Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus, with the therapeutic index 9.69. In vivo, berberine decreased mice mortality from 90% to 55%, reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50 ± 0.67, 4.50 ± 1.00, and 5.50 ± 1.00 in the berberine group on days 2, 4, and 6, respectively, which were significantly reduced compared to 2.17 ± 0.22, 6.83 ± 0.44, and 8.50 ± 0.33 in the infected group (P<0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group (P<0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 (P<0.01) and day 6 (P<0.05), and those of MCP-1 were inhibited on day 6 (P<0.01) compared with the infected group. Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo. The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection, as well as improving the pathogenic changes by repressing inflammatory substances release.
Author 吴莹 李季倩 金叶智 吴珺 王谦 郝鈺
AuthorAffiliation Key Laboratory of Chinese Medicine on Viral Diseases,Ministry of Education,Microbiology and Immunology Department,Beijing University of Chinese Medicine,Beijing(100029),China
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Issue 6
Keywords influenza virus
antiviral effects
berberine
Language English
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Notes 11-4928/R
in vivo ;in vitro ;antiviral effects ;berberine ;influenza virus
Objective:To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods:In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells.In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice.The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality.On days 2,4,and 6,the viral titers in the lungs were determined by hemagglutination assay;hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha(TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay;the concentrations of nitric oxide(NO) and inducible nitric oxide synthetase(iNOS) were detected by colorimetric method;reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-αand MCP-1.Results: Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus,with the therapeutic index 9.69.In vivo,berberine decreased mice mortality from 90%to 55%,reduced virus titers in the lungs on day 2 postinfection(P0.05).The lung histology scores were 1.50±0.67,4.50±1.00,and 5.50±1.00 in the berberine group on days 2,4,and 6,respectively,which were significantly reduced compared to 2.17±0.22,6.83±0.44,and 8.50±0.33 in the infected group(P0.05).The productions of NO and iNOS were repressed by berberine compared with those in the infected group(P0.01).The transcription and expression of TNF-αwere inhibited by berberine on day 4(P0.01) and day 6(P0.05),and those of MCP-1 were inhibited on day 6(P0.01) compared with the infected group.Conclusions:Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo.The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection,as well as improving the pathogenic changes by repressing inflammatory substances release
WU Ying ,LI Ji-qian,KIM Ye-ji, WU Jun, WANG Qian, HAO Yu (Key Laboratory of Chinese Medicine on Viral Diseases,Ministry of Education,Microbiology and Immunology Department,Beijing University of Chinese Medicine,Beijing(100029),China )
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PublicationTitle Chinese journal of integrative medicine
PublicationTitleAbbrev Chin. J. Integr. Med
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Snippet Objective:To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods:In vitro anti-influenza virus assays...
Objective To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo . Methods In vitro anti-influenza virus assays...
To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo. In vitro anti-influenza virus assays were performed by...
To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.OBJECTIVETo explore the potential effects of berberine on...
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SubjectTerms Animals
Antiviral Agents - pharmacology
Antiviral Agents - therapeutic use
Berberine - pharmacology
Berberine - therapeutic use
Cell Line
Chemokine CCL2 - genetics
Chemokine CCL2 - metabolism
Dogs
Female
Lung - drug effects
Lung - enzymology
Lung - pathology
Lung - virology
Male
Medicine
Medicine & Public Health
Mice
Neuraminidase - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - metabolism
Original Article
Orthomyxoviridae - drug effects
Orthomyxoviridae - enzymology
Orthomyxoviridae Infections - complications
Orthomyxoviridae Infections - drug therapy
Orthomyxoviridae Infections - pathology
Orthomyxoviridae Infections - virology
Pneumonia - complications
Pneumonia - drug therapy
Pneumonia - pathology
Pneumonia - virology
Reactive Oxygen Species - metabolism
Survival Analysis
Transcription, Genetic - drug effects
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
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Title In Vivo and in Vitro Antiviral Effects of Berberine on Influenza Virus
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