Elucidation of Antifungal Metabolites Produced by Pseudomonas aurantiaca IB5-10 with Broad-Spectrum Antifungal Activity

• Published in: Journal of microbiology and biotechnology Vol. 22; no. 3; pp. 326 - 330
• Main Authors: Park, G.K., Yeungnam University, Gyeongsan, Republic of Korea, Lim, J.H., Yeungnam University, Gyeongsan, Republic of Korea, Kim, S.D., Yeungnam University, Gyeongsan, Republic of Korea, Shim, S.H., Yeungnam University, Gyeongsan, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.03.2012; 한국미생물·생명공학회
• Subjects: antifungal activities; Antifungal Agents - chemistry; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Fungi - drug effects; Fungi - physiology; Microbial Sensitivity Tests; PCA; Phenazines - chemistry; Phenazines - metabolism; Phenazines - pharmacology; Plant Diseases - microbiology; PSEUDOMONAS; Pseudomonas - chemistry; Pseudomonas - metabolism; Pseudomonas aurantiaca; 생물학; Pseudomonadales; cyclo-(L-Pro-L-Val); 2-hydroxyphenazine (2-OH-PHZ); Antifungal agent; phenazine-1-carboxylic acid (PCA); Carboxylic acid; Metabolite; antifungal activities; Bacteria; Pseudomonadaceae; Pseudomonas aurarrtiaca; Pseudomonas
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1106.06042

Antifungal metabolites were isolated from a culture of Pseudomonas aurantiaca IBS-10. Chemical structures of the metabolites were elucidated as phenazine-1-carboxylic acid (PCA; 1), 2-hydroxyphenazine (2-OH-PHZ; 2), and cyclo-(L-Pro-L-Val; 3), respectively, based on spectroscopic methods. Among them, 3 was isolated for the first time from this strain. The antifungal activities of 1-3 were evaluated against a variety of plant pathogens. To the best of our knowledge, the antifungal activities of 3 against plant fungal pathogens have been evaluated for the first time in this work. PCA (1) showed the most potent antifungal activities against Phytophthora capsici, Rhizoctonia solani AG-1(IA), and Pythium ultimum with MICs (㎍/ml) of less than 1.0, 1.3, and 2.0, respectively. On the other hand, 2-OH-PHZ (2) showed potent antifungal activity against R. solani AG-1(IA) with the MIC (㎍/ml) of 2.0, whereas it showed moderate antifungal activity against P. ultimum with the MIC (㎍/ml) of 50.0. In addition, 3 showed antifungal activity against only R. solani AG-1(IA).