Comparative genomics of Cryptosporidium parvum reveals the emergence of an outbreak-associated population in Europe and its spread to the United States
The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gp60 gene enabled the classification of C. parvum isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Euro...
Saved in:
| Published in | Genome research Vol. 34; no. 6; pp. 877 - 887 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Cold Spring Harbor Laboratory Press
01.06.2024
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1088-9051 1549-5469 1549-5469 |
| DOI | 10.1101/gr.278830.123 |
Cover
| Abstract | The zoonotic parasite
Cryptosporidium parvum
is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic
gp60
gene enabled the classification of
C. parvum
isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified. |
|---|---|
| AbstractList | The zoonotic parasite
is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic
gene enabled the classification of
isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified. The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gp60 gene enabled the classification of C. parvum isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified. The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gp60 gene enabled the classification of C. parvum isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified. The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gp60 gene enabled the classification of C. parvum isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified.The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gp60 gene enabled the classification of C. parvum isolates into multiple groups (e.g., IIa, IIc, Id) and a large number of subtypes. In Europe, subtype IIaA15G2R1 is largely predominant and has been associated with many water- and food-borne outbreaks. In this study, we generated new whole-genome sequence (WGS) data from 123 human- and ruminant-derived isolates collected in 13 European countries and included other available WGS data from Europe, Egypt, China, and the United States (n = 72) in the largest comparative genomics study to date. We applied rigorous filters to exclude mixed infections and analyzed a data set from 141 isolates from the zoonotic groups IIa (n = 119) and IId (n = 22). Based on 28,047 high-quality, biallelic genomic SNPs, we identified three distinct and strongly supported populations: Isolates from China (IId) and Egypt (IIa and IId) formed population 1; a minority of European isolates (IIa and IId) formed population 2; and the majority of European (IIa, including all IIaA15G2R1 isolates) and all isolates from the United States (IIa) clustered in population 3. Based on analyses of the population structure, population genetics, and recombination, we show that population 3 has recently emerged and expanded throughout Europe to then, possibly from the United Kingdom, reach the United States, where it also expanded. The reason(s) for the successful spread of population 3 remain elusive, although genes under selective pressure uniquely in this population were identified. |
| Author | Chalmers, Rachel Castelli, Michele Klotz, Christian Nardi, Tiago Sassera, Davide Robinson, Guy Blanchard, Yannick Sroka, Jacek Rimhanen-Finne, Ruska Betson, Martha Vatta, Paolo Zalewska, Barbora Bellinzona, Greta D'Avino, Nicoletta Bandi, Claudio Östlund, Emma Batisti Biffignandi, Gherard Stensvold, Christen Rune Gomes, Jacinto Cacciò, Simone M. Sannella, Anna Rosa Troell, Karin Plutzer, Judith Davidson, Rebecca Bujila, Ioana Adjou, Karim Enbom, Tuulia Karadjian, Gregory |
| AuthorAffiliation | 3 Department of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford GU2 7AL, United Kingdom 13 Swedish Veterinary Agency, SE-751 89 Uppsala, Sweden 11 National Institute for Agricultural and Veterinary Research, 1300 Lisbon, Portugal 16 Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy 14 National Institute for Public Education, Budapest, 1007, Hungary 5 Department of Microbiology, Public Health Agency of Sweden, SE-171 82 Solna, Sweden 6 Cryptosporidium Reference Unit, Public Health Wales, Swansea SA2 8QA, United Kingdom 9 Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, 06126 Perugia, Italy 8 Norwegian Veterinary Institute, N-1431 Ås, Norway 1 Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy 18 Statens Serum Institut, 2300 Copenhagen, Denmark 2 UMR BIPAR, Anses, Laboratoire de Santé Animale, INRAE, École Nationale Vétérinaire d'Alfort, 94700 Maisons-Alfort, France 20 Depa |
| AuthorAffiliation_xml | – name: 2 UMR BIPAR, Anses, Laboratoire de Santé Animale, INRAE, École Nationale Vétérinaire d'Alfort, 94700 Maisons-Alfort, France – name: 7 Swansea Medical School, Swansea University, Swansea SA2 8PP, United Kingdom – name: 21 IRCCS Fondazione Policlinico San Matteo, 27100 Pavia, Italy – name: 19 Veterinary Research Institute, Department of Food and Feed Safety, 621 00 Brno, Czech Republic – name: 18 Statens Serum Institut, 2300 Copenhagen, Denmark – name: 9 Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, 06126 Perugia, Italy – name: 15 Finnish Institute for Health and Welfare, FI-00271 Helsinki, Finland – name: 17 Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland – name: 20 Department of Biosciences, University of Milan, 20133 Milan, Italy – name: 6 Cryptosporidium Reference Unit, Public Health Wales, Swansea SA2 8QA, United Kingdom – name: 14 National Institute for Public Education, Budapest, 1007, Hungary – name: 3 Department of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford GU2 7AL, United Kingdom – name: 1 Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy – name: 8 Norwegian Veterinary Institute, N-1431 Ås, Norway – name: 16 Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy – name: 4 Viral Genetics and Biosecurity Unit (GVB), French Agency for Food, Environmental and Occupational Health Safety (ANSES), 22440 Ploufragan, France – name: 11 National Institute for Agricultural and Veterinary Research, 1300 Lisbon, Portugal – name: 10 Animal Health Diagnostic Unit, Finnish Food Authority, FI-70210 Kuopio, Finland – name: 12 Department of Infectious Diseases, Robert Koch Institute, 13353 Berlin, Germany – name: 13 Swedish Veterinary Agency, SE-751 89 Uppsala, Sweden – name: 5 Department of Microbiology, Public Health Agency of Sweden, SE-171 82 Solna, Sweden |
| Author_xml | – sequence: 1 givenname: Greta orcidid: 0000-0001-7050-8940 surname: Bellinzona fullname: Bellinzona, Greta – sequence: 2 givenname: Tiago orcidid: 0000-0002-1248-9873 surname: Nardi fullname: Nardi, Tiago – sequence: 3 givenname: Michele orcidid: 0000-0003-2177-5772 surname: Castelli fullname: Castelli, Michele – sequence: 4 givenname: Gherard surname: Batisti Biffignandi fullname: Batisti Biffignandi, Gherard – sequence: 5 givenname: Karim surname: Adjou fullname: Adjou, Karim – sequence: 6 givenname: Martha surname: Betson fullname: Betson, Martha – sequence: 7 givenname: Yannick surname: Blanchard fullname: Blanchard, Yannick – sequence: 8 givenname: Ioana surname: Bujila fullname: Bujila, Ioana – sequence: 9 givenname: Rachel surname: Chalmers fullname: Chalmers, Rachel – sequence: 10 givenname: Rebecca surname: Davidson fullname: Davidson, Rebecca – sequence: 11 givenname: Nicoletta surname: D'Avino fullname: D'Avino, Nicoletta – sequence: 12 givenname: Tuulia surname: Enbom fullname: Enbom, Tuulia – sequence: 13 givenname: Jacinto surname: Gomes fullname: Gomes, Jacinto – sequence: 14 givenname: Gregory surname: Karadjian fullname: Karadjian, Gregory – sequence: 15 givenname: Christian surname: Klotz fullname: Klotz, Christian – sequence: 16 givenname: Emma surname: Östlund fullname: Östlund, Emma – sequence: 17 givenname: Judith surname: Plutzer fullname: Plutzer, Judith – sequence: 18 givenname: Ruska surname: Rimhanen-Finne fullname: Rimhanen-Finne, Ruska – sequence: 19 givenname: Guy surname: Robinson fullname: Robinson, Guy – sequence: 20 givenname: Anna Rosa surname: Sannella fullname: Sannella, Anna Rosa – sequence: 21 givenname: Jacek surname: Sroka fullname: Sroka, Jacek – sequence: 22 givenname: Christen Rune surname: Stensvold fullname: Stensvold, Christen Rune – sequence: 23 givenname: Karin surname: Troell fullname: Troell, Karin – sequence: 24 givenname: Paolo surname: Vatta fullname: Vatta, Paolo – sequence: 25 givenname: Barbora surname: Zalewska fullname: Zalewska, Barbora – sequence: 26 givenname: Claudio surname: Bandi fullname: Bandi, Claudio – sequence: 27 givenname: Davide orcidid: 0000-0003-0442-5252 surname: Sassera fullname: Sassera, Davide – sequence: 28 givenname: Simone M. surname: Cacciò fullname: Cacciò, Simone M. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38977307$$D View this record in MEDLINE/PubMed https://hal.science/hal-04643965$$DView record in HAL |
| BookMark | eNp1kk1v1DAQhiNURD_gyBVZ4gKHFDuxY-eEqlWhSCtxgJ4tx5nsuiR2sJ2V-kv4u8x2SwUrcRp_PO87Hs-cFyc-eCiK14xeMkbZh028rKRSNW6r-llxxgRvS8Gb9gTXVKmypYKdFucp3VFKa67Ui-K0Vq2UNZVnxa9VmGYTTXY7IBvwYXI2kTCQVbyfc0hziK53y0QQ2mGIsAMzJpK3QGCCiBILe954EpbcRTA_SpNSsM5k6Mkc5mVE9-CJ8-R6iWEGZHviciJpRrwnOTzY3Xq3V3zLKEwvi-cD5oFXj_GiuP10_X11U66_fv6yulqXljOWS2ls11QC-k71DRdAbWN5XfFKcj6oivW07Q0b5KCgbatOAGMAneksNaKx0tQXxceD77x0E_QWfI5m1HN0k4n3Ohin_73xbqs3YacZq9paiAod3h8ctke6m6u13p9R3vC6bcSOIfvuMVsMPxdIWU8uWRhH4yEsSWNLJJNC0T369gi9C0v0-BdItYzJBgtC6s3fz3_K_6fBCJQHwMaQUoThCWFU7wdIb6I-DJDGAUK-PuKtyw_9w-rd-B_Vb6TvzE8 |
| CitedBy_id | crossref_primary_10_1016_j_jinf_2025_106427 |
| Cites_doi | 10.1016/j.cub.2021.04.028 10.1038/nmeth.1923 10.1038/s41598-023-39518-3) 10.1016/S2214-109X(18)30283-3 10.1016/j.ijpara.2021.08.007 10.1016/j.meegid.2019.05.018 10.1016/j.chom.2022.11.013 10.1073/pnas.1303367110 10.1371/journal.pone.0012901 10.7554/eLife.65088 10.3168/jds.S0022-0302(42)95275-5 10.1007/978-3-319-24277-4_9 10.1093/molbev/msj030 10.1093/nar/gkh050 10.3390/ani11113307 10.1093/nar/gkl200 10.1093/molbev/msz189 10.1093/bioinformatics/btu033 10.12688/f1000research.15333.1 10.1093/nar/gkh340 10.1038/srep04208 10.3389/fmicb.2021.810142 10.1016/j.pt.2019.12.016 10.1111/1755-0998.13721 10.1128/MRA.01291-18 10.1128/mSystems.00920-20 10.1186/s13071-023-05844-8 10.1186/s12864-015-1805-9 10.1073/pnas.0509210103 10.1093/bioinformatics/btr330 10.2807/1560-7917.ES.2023.28.43.2300538 10.1093/bioinformatics/btu170 10.1093/gigascience/giab008 10.1093/bioinformatics/bty875 10.1086/702788 10.1186/s12936-018-2349-7 10.1016/j.meegid.2022.105212 10.1016/S0140-6736(13)60844-2 10.1038/ng.806 10.1080/14787210.2018.1500457 10.1111/mec.16556 10.1590/S1678-9946202264070 10.3389/fcimb.2023.1115522 10.1128/AEM.69.7.4302-4307.2003 10.1016/j.ijpara.2016.12.002 10.1016/j.pt.2018.07.009 10.1126/science.1170587 10.1093/ve/vev003 10.1128/JCM.41.6.2744-2747.2003 10.1186/s13643-018-0731-8 10.1111/j.1471-8286.2005.01155.x 10.1371/journal.pcbi.1005595 10.1093/molbev/msv035 10.7554/eLife.70451 10.1016/j.cmi.2016.04.021 10.1017/S0031182016001323 10.1186/s13059-019-1832-y 10.1016/j.actatropica.2021.106273 10.1038/s41564-019-0377-x 10.1186/s12864-016-2815-y 10.1038/nature11174 10.1038/s41467-017-02341-2 10.1093/molbev/msac150 10.1016/j.rvsc.2020.08.002 10.1186/s12864-019-5788-9 10.1101/gr.275325.121 |
| ContentType | Journal Article |
| Copyright | 2024 Bellinzona et al.; Published by Cold Spring Harbor Laboratory Press. Copyright Cold Spring Harbor Laboratory Press Jun 2024 Attribution 2024 |
| Copyright_xml | – notice: 2024 Bellinzona et al.; Published by Cold Spring Harbor Laboratory Press. – notice: Copyright Cold Spring Harbor Laboratory Press Jun 2024 – notice: Attribution – notice: 2024 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7TM 8FD FR3 P64 RC3 7X8 1XC 5PM |
| DOI | 10.1101/gr.278830.123 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Nucleic Acids Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic Hyper Article en Ligne (HAL) PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Genetics Abstracts Engineering Research Database Technology Research Database Nucleic Acids Abstracts Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE Genetics Abstracts CrossRef MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry Biology |
| DocumentTitleAlternate | Bellinzona et al |
| EISSN | 1549-5469 |
| EndPage | 887 |
| ExternalDocumentID | PMC11293552 oai_HAL_hal_04643965v1 38977307 10_1101_gr_278830_123 |
| Genre | Journal Article |
| GeographicLocations | United States Europe China Egypt United States--US |
| GeographicLocations_xml | – name: China – name: United States – name: Egypt – name: Europe – name: United States--US |
| GrantInformation_xml | – fundername: European Union's Horizon 2020 Research and Innovation grantid: 773830 – fundername: Ministry of Agriculture and Forestry of Finland – fundername: Development Fund Agricultural and Forestry MAKERA |
| GroupedDBID | --- .GJ 18M 29H 2WC 39C 4.4 53G 5GY 5RE 5VS AAFWJ AAYOK AAYXX AAZTW ABDIX ABDNZ ACGFO ACLKE ACYGS ADBBV ADNWM AEILP AENEX AHPUY AI. ALMA_UNASSIGNED_HOLDINGS BAWUL BTFSW C1A CITATION CS3 DIK DU5 E3Z EBS EJD F5P FRP GX1 H13 HYE IH2 K-O KQ8 MV1 R.V RCX RHI RNS RPM RXW SJN TAE TR2 VH1 W8F WOQ YKV ZCG ZGI ZXP CGR CUY CVF ECM EIF NPM 7TM 8FD FR3 P64 RC3 7X8 1XC 5PM |
| ID | FETCH-LOGICAL-c411t-7acb625edb8d645e0c6c43242744f821d09da1f7f8e992b5e11eebabc0a56c7a3 |
| ISSN | 1088-9051 1549-5469 |
| IngestDate | Thu Aug 21 18:33:37 EDT 2025 Fri Sep 12 12:49:05 EDT 2025 Fri Sep 05 06:42:44 EDT 2025 Mon Jun 30 17:05:30 EDT 2025 Mon Jul 21 06:04:24 EDT 2025 Tue Jul 01 02:20:48 EDT 2025 Thu Apr 24 23:01:21 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Language | English |
| License | 2024 Bellinzona et al.; Published by Cold Spring Harbor Laboratory Press. Attribution: http://creativecommons.org/licenses/by This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c411t-7acb625edb8d645e0c6c43242744f821d09da1f7f8e992b5e11eebabc0a56c7a3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 PMCID: PMC11293552 |
| ORCID | 0000-0003-0442-5252 0000-0002-1248-9873 0000-0003-2177-5772 0000-0001-7050-8940 0000-0002-4711-6671 0000-0001-8224-521X 0000-0003-4043-0876 |
| OpenAccessLink | https://pubmed.ncbi.nlm.nih.gov/PMC11293552 |
| PMID | 38977307 |
| PQID | 3091176992 |
| PQPubID | 2049132 |
| PageCount | 11 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_11293552 hal_primary_oai_HAL_hal_04643965v1 proquest_miscellaneous_3077175801 proquest_journals_3091176992 pubmed_primary_38977307 crossref_primary_10_1101_gr_278830_123 crossref_citationtrail_10_1101_gr_278830_123 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2024-06-01 |
| PublicationDateYYYYMMDD | 2024-06-01 |
| PublicationDate_xml | – month: 06 year: 2024 text: 2024-06-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: New York |
| PublicationTitle | Genome research |
| PublicationTitleAlternate | Genome Res |
| PublicationYear | 2024 |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher_xml | – name: Cold Spring Harbor Laboratory Press |
| References | 2024072312051061000_34.6.877.1 2024072312051061000_34.6.877.2 2024072312051061000_34.6.877.3 2024072312051061000_34.6.877.4 2024072312051061000_34.6.877.38 2024072312051061000_34.6.877.5 2024072312051061000_34.6.877.37 2024072312051061000_34.6.877.6 2024072312051061000_34.6.877.7 2024072312051061000_34.6.877.39 2024072312051061000_34.6.877.8 2024072312051061000_34.6.877.9 2024072312051061000_34.6.877.30 2024072312051061000_34.6.877.32 2024072312051061000_34.6.877.31 2024072312051061000_34.6.877.34 2024072312051061000_34.6.877.33 2024072312051061000_34.6.877.36 2024072312051061000_34.6.877.35 2024072312051061000_34.6.877.50 2024072312051061000_34.6.877.49 2024072312051061000_34.6.877.48 2024072312051061000_34.6.877.41 2024072312051061000_34.6.877.40 2024072312051061000_34.6.877.43 2024072312051061000_34.6.877.42 2024072312051061000_34.6.877.45 2024072312051061000_34.6.877.44 2024072312051061000_34.6.877.47 2024072312051061000_34.6.877.46 2024072312051061000_34.6.877.61 2024072312051061000_34.6.877.60 2024072312051061000_34.6.877.16 2024072312051061000_34.6.877.15 2024072312051061000_34.6.877.59 2024072312051061000_34.6.877.18 2024072312051061000_34.6.877.17 2024072312051061000_34.6.877.19 2024072312051061000_34.6.877.52 2024072312051061000_34.6.877.51 2024072312051061000_34.6.877.10 2024072312051061000_34.6.877.54 2024072312051061000_34.6.877.53 2024072312051061000_34.6.877.12 2024072312051061000_34.6.877.56 2024072312051061000_34.6.877.11 2024072312051061000_34.6.877.55 2024072312051061000_34.6.877.14 2024072312051061000_34.6.877.58 2024072312051061000_34.6.877.13 2024072312051061000_34.6.877.57 2024072312051061000_34.6.877.27 2024072312051061000_34.6.877.26 2024072312051061000_34.6.877.29 2024072312051061000_34.6.877.28 2024072312051061000_34.6.877.63 2024072312051061000_34.6.877.62 2024072312051061000_34.6.877.21 2024072312051061000_34.6.877.65 2024072312051061000_34.6.877.20 2024072312051061000_34.6.877.64 2024072312051061000_34.6.877.23 2024072312051061000_34.6.877.67 2024072312051061000_34.6.877.22 2024072312051061000_34.6.877.66 2024072312051061000_34.6.877.25 2024072312051061000_34.6.877.69 2024072312051061000_34.6.877.24 2024072312051061000_34.6.877.68 |
| References_xml | – ident: 2024072312051061000_34.6.877.42 doi: 10.1016/j.cub.2021.04.028 – ident: 2024072312051061000_34.6.877.38 doi: 10.1038/nmeth.1923 – ident: 2024072312051061000_34.6.877.62 – ident: 2024072312051061000_34.6.877.17 doi: 10.1038/s41598-023-39518-3) – ident: 2024072312051061000_34.6.877.35 doi: 10.1016/S2214-109X(18)30283-3 – ident: 2024072312051061000_34.6.877.54 doi: 10.1016/j.ijpara.2021.08.007 – ident: 2024072312051061000_34.6.877.8 doi: 10.1016/j.meegid.2019.05.018 – ident: 2024072312051061000_34.6.877.31 doi: 10.1016/j.chom.2022.11.013 – ident: 2024072312051061000_34.6.877.44 doi: 10.1073/pnas.1303367110 – ident: 2024072312051061000_34.6.877.59 doi: 10.1371/journal.pone.0012901 – ident: 2024072312051061000_34.6.877.3 doi: 10.7554/eLife.65088 – ident: 2024072312051061000_34.6.877.7 doi: 10.3168/jds.S0022-0302(42)95275-5 – ident: 2024072312051061000_34.6.877.66 doi: 10.1007/978-3-319-24277-4_9 – ident: 2024072312051061000_34.6.877.32 doi: 10.1093/molbev/msj030 – ident: 2024072312051061000_34.6.877.50 doi: 10.1093/nar/gkh050 – ident: 2024072312051061000_34.6.877.55 doi: 10.3390/ani11113307 – ident: 2024072312051061000_34.6.877.58 doi: 10.1093/nar/gkl200 – ident: 2024072312051061000_34.6.877.16 doi: 10.1093/molbev/msz189 – ident: 2024072312051061000_34.6.877.57 doi: 10.1093/bioinformatics/btu033 – ident: 2024072312051061000_34.6.877.52 – ident: 2024072312051061000_34.6.877.5 doi: 10.12688/f1000research.15333.1 – ident: 2024072312051061000_34.6.877.20 doi: 10.1093/nar/gkh340 – ident: 2024072312051061000_34.6.877.63 doi: 10.1038/srep04208 – ident: 2024072312051061000_34.6.877.27 doi: 10.3389/fmicb.2021.810142 – ident: 2024072312051061000_34.6.877.33 doi: 10.1016/j.pt.2019.12.016 – ident: 2024072312051061000_34.6.877.29 doi: 10.1111/1755-0998.13721 – ident: 2024072312051061000_34.6.877.47 doi: 10.1128/MRA.01291-18 – ident: 2024072312051061000_34.6.877.26 doi: 10.1128/mSystems.00920-20 – ident: 2024072312051061000_34.6.877.61 doi: 10.1186/s13071-023-05844-8 – ident: 2024072312051061000_34.6.877.28 doi: 10.1186/s12864-015-1805-9 – ident: 2024072312051061000_34.6.877.4 doi: 10.1073/pnas.0509210103 – ident: 2024072312051061000_34.6.877.14 doi: 10.1093/bioinformatics/btr330 – ident: 2024072312051061000_34.6.877.48 doi: 10.2807/1560-7917.ES.2023.28.43.2300538 – ident: 2024072312051061000_34.6.877.6 doi: 10.1093/bioinformatics/btu170 – ident: 2024072312051061000_34.6.877.15 doi: 10.1093/gigascience/giab008 – ident: 2024072312051061000_34.6.877.69 doi: 10.1093/bioinformatics/bty875 – ident: 2024072312051061000_34.6.877.24 doi: 10.1086/702788 – ident: 2024072312051061000_34.6.877.56 doi: 10.1186/s12936-018-2349-7 – ident: 2024072312051061000_34.6.877.13 – ident: 2024072312051061000_34.6.877.30 doi: 10.1016/j.meegid.2022.105212 – ident: 2024072312051061000_34.6.877.37 doi: 10.1016/S0140-6736(13)60844-2 – ident: 2024072312051061000_34.6.877.18 doi: 10.1038/ng.806 – ident: 2024072312051061000_34.6.877.10 doi: 10.1080/14787210.2018.1500457 – ident: 2024072312051061000_34.6.877.12 doi: 10.1111/mec.16556 – ident: 2024072312051061000_34.6.877.34 doi: 10.1590/S1678-9946202264070 – ident: 2024072312051061000_34.6.877.36 doi: 10.3389/fcimb.2023.1115522 – ident: 2024072312051061000_34.6.877.53 doi: 10.1128/AEM.69.7.4302-4307.2003 – ident: 2024072312051061000_34.6.877.22 doi: 10.1016/j.ijpara.2016.12.002 – ident: 2024072312051061000_34.6.877.23 doi: 10.1016/j.pt.2018.07.009 – ident: 2024072312051061000_34.6.877.11 doi: 10.1126/science.1170587 – ident: 2024072312051061000_34.6.877.41 doi: 10.1093/ve/vev003 – ident: 2024072312051061000_34.6.877.1 doi: 10.1128/JCM.41.6.2744-2747.2003 – ident: 2024072312051061000_34.6.877.43 doi: 10.1186/s13643-018-0731-8 – ident: 2024072312051061000_34.6.877.49 doi: 10.1111/j.1471-8286.2005.01155.x – ident: 2024072312051061000_34.6.877.65 doi: 10.1371/journal.pcbi.1005595 – ident: 2024072312051061000_34.6.877.45 doi: 10.1093/molbev/msv035 – ident: 2024072312051061000_34.6.877.19 doi: 10.7554/eLife.70451 – ident: 2024072312051061000_34.6.877.9 doi: 10.1016/j.cmi.2016.04.021 – ident: 2024072312051061000_34.6.877.25 doi: 10.1017/S0031182016001323 – ident: 2024072312051061000_34.6.877.21 doi: 10.1186/s13059-019-1832-y – ident: 2024072312051061000_34.6.877.51 doi: 10.1016/j.actatropica.2021.106273 – ident: 2024072312051061000_34.6.877.46 doi: 10.1038/s41564-019-0377-x – ident: 2024072312051061000_34.6.877.60 doi: 10.1186/s12864-016-2815-y – ident: 2024072312051061000_34.6.877.40 doi: 10.1038/nature11174 – ident: 2024072312051061000_34.6.877.39 doi: 10.1038/s41467-017-02341-2 – ident: 2024072312051061000_34.6.877.64 doi: 10.1093/molbev/msac150 – ident: 2024072312051061000_34.6.877.68 doi: 10.1016/j.rvsc.2020.08.002 – ident: 2024072312051061000_34.6.877.67 doi: 10.1186/s12864-019-5788-9 – ident: 2024072312051061000_34.6.877.2 doi: 10.1101/gr.275325.121 |
| SSID | ssj0003488 |
| Score | 2.4717956 |
| Snippet | The zoonotic parasite
Cryptosporidium parvum
is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic... The zoonotic parasite is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic gene enabled the... The zoonotic parasite Cryptosporidium parvum is a global cause of gastrointestinal disease in humans and ruminants. Sequence analysis of the highly polymorphic... |
| SourceID | pubmedcentral hal proquest pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 877 |
| SubjectTerms | Animals China - epidemiology Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidium parvum Cryptosporidium parvum - genetics Disease Outbreaks Egypt - epidemiology Epidemics Europe - epidemiology Gastrointestinal diseases Genome, Protozoan Genomic analysis Genomics Genomics - methods Humans Life Sciences Nucleotide sequence Outbreaks Phylogeny Polymorphism, Single Nucleotide Population genetics Population structure Sequence analysis Single-nucleotide polymorphism United States - epidemiology Whole Genome Sequencing - methods Zoonoses |
| Title | Comparative genomics of Cryptosporidium parvum reveals the emergence of an outbreak-associated population in Europe and its spread to the United States |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38977307 https://www.proquest.com/docview/3091176992 https://www.proquest.com/docview/3077175801 https://hal.science/hal-04643965 https://pubmed.ncbi.nlm.nih.gov/PMC11293552 |
| Volume | 34 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF61RUAvCFoKgRYtCPUSXLJ--5gWmgChQqKVerPW9ia1aOwodSq1f4S_y8w-HKcJUuHiRPbIWWcenpmd-YaQ9xziHqxOtALGI8u1RWYlDmOWHfLQ9cKUBy42J38_8ftn7tdz73xtfa9RtTSrkoP0dmVfyf9wFc4BX7FL9h84W98UTsB34C8cgcNwvBePjxrQ3Qi2OkbIZayvmN5MqhID1jzLZ-M2EF3DB6I1IVqyrOPQbZcyZYDjKeD_AP_xl8U1v8ARndTDvTArovL29WbD1QTIs5Wua9Ph7eGycDRLI2um24Ly4rZUHWk9LHqs09Ios0qM-Kic75FcSexQU-ovLuflANigUeXtQ0w_jTArLql62FmmIANMWsN25-VXqrqovIRFy9wmtjOBPrQHSiuw9KBZnoJWG0ylhUBj6qWmLbkbWZ6r5sAYU6_zpvmS3Q71LBntAigfYPntIqcajKYHdhCGDiJzOE06EI7JWIoaeIEBmM5g_pI1hQX97s_4x6fjePDl5Nvi1Rrsu98dxBcgi7jp7ES-dw3B_QM7ANfQJKO0o-G4oer01E9fQ8iyjwtr3CSPzIIWvK_1C6z9XQ6s7tYHNxyu06fkiY6UaFeJ_TOyJootst0tgDfjG7pPZe2y3BTaIg8PzbfHR2aC4Tb53dAPavSDlkN6Rz-o0g-q9YOCQNNaP5CeF3SFftC5ftC8oEo_gDajoB9U6QetSnk7pR9U6cdzcnb8-fSob-lBJFbqMlZZAU8T3_ZEloSZ73qik_opIlkiuuYwtFnWiTLOhsEwFFFkJ55gTIiEJ2mHe34acGeHbBRlIV4SCq6Rl0ZM4BwSF7zHxM58n9tOEoVO4ridFvlgGBSnGqUfh8VcxjJa77B4NI0Va2NgbYvs1-QTBU_zN8J3KFOGZrWctciuEYZYm7mr2IGIggU-PFiLvK0vAydxZ5EXopwhTRBAHALebou8ULJT_5QRvRYJF6RqYS2LV4r8QgLdYywI8ZD96j6Lf00253Zkl2xU05nYg4ChSt5ItfkDaTYcLA |
| linkProvider | National Library of Medicine |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Comparative+genomics+of+Cryptosporidium+parvum+reveals+the+emergence+of+an+outbreak-associated+population+in+Europe+and+its+spread+to+the+United+States&rft.jtitle=Genome+research&rft.au=Bellinzona%2C+Greta&rft.au=Nardi%2C+Tiago&rft.au=Castelli%2C+Michele&rft.au=Batisti+Biffignandi%2C+Gherard&rft.date=2024-06-01&rft.pub=Cold+Spring+Harbor+Laboratory+Press&rft.issn=1088-9051&rft.eissn=1549-5469&rft.volume=34&rft.issue=6&rft.spage=877&rft.epage=887&rft_id=info:doi/10.1101%2Fgr.278830.123&rft_id=info%3Apmid%2F38977307&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=oai_HAL_hal_04643965v1 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1088-9051&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1088-9051&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1088-9051&client=summon |