Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

• Published in: Chinese journal of oceanology and limnology Vol. 34; no. 4; pp. 719 - 729
• Main Author: 周君 侯付景 李晔 苏秀榕 李太武 金春华
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.07.2016; Springer Nature B.V
• Subjects: Acaudina; amino acid sequences; Amino acids; Antibodies; Aquaculture; Aquatic life; Bioinformatics; Biology; cDNA libraries; cDNA文库; Complementary DNA; Cytology; defense mechanisms; Earth and Environmental Science; Earth Sciences; Energy metabolism; Ferritin; Gene expression; gene expression regulation; Genes; Genetics; Holothurioidea; Homology; Intestine; Intestines; Marine; Marine invertebrates; messenger RNA; Metabolism; mitochondria; Nucleotide sequence; Oceanography; PCR; Polyclonal antibodies; Polyculture (aquaculture); Polymerase chain reaction; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; Signal transduction; Transduction; 分子遗传学; 半定量RT-PCR; 地瓜; 差异表达; 海参; 蛋白肽; 铁蛋白基因; China, People's Rep; ferritin; real-time RT-PCR; polyclonal antibody; cDNA library
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-016-5010-8

Acaudina leucoprocta is an edible sea cucumber of economic interest that is widely distributed in China. Little information is available concerning the molecular genetics of this species although such knowledge would contribute to a better understanding of the optimal conditions for its aquaculture and its mechanisms of defense against disease. Therefore, we constructed a c DNA library and, based on bioinformatics analysis of the sequences, the functions of 75% of the c DNAs were identifi ed, including those involved in cell structure, energy metabolism, mitochondrial function, and signal transduction pathways. Approximately 25% of genes in the library were unmatched. The gene for A. leucoprocta ferritin was also cloned. The predicted amino-acid sequence of ferritin displayed signifi cant homology with other sea-cucumber counterparts but indicated that it was a new member of the ferritin family. Semiquantitative real-time RT-PCR indicated the highest levels of ferritin m RNA expression in the intestine. A polyclonal antibody of ferritin was also produced. These data provide a set of molecular tools essential for further studies of the functions of ferritin protein in A. leucoprocta.