Response surface modeling and process optimization of aqueous extraction of natural pigments from Beta vulgaris using Box–Behnken design of experiments

The aim of the study was to investigate the influence of independent process variables like extraction temperature, time and mass of beetroot on the aqueous extraction of betalain from beetroot. The optimum conditions for the aqueous extraction of betalamic acid, betaxanthin and betacyanin from beet...

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Published inDyes and pigments Vol. 111; pp. 64 - 74
Main Authors Swamy, Gabriela John, Sangamithra, A., Chandrasekar, V.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.12.2014
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Online AccessGet full text
ISSN0143-7208
1873-3743
DOI10.1016/j.dyepig.2014.05.028

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Abstract The aim of the study was to investigate the influence of independent process variables like extraction temperature, time and mass of beetroot on the aqueous extraction of betalain from beetroot. The optimum conditions for the aqueous extraction of betalamic acid, betaxanthin and betacyanin from beetroot were performed using a three-factor and three-level Box–Behnken design under response surface methodology. The pigments were extracted from beetroot at temperature (40–70 °C), time (30–90 min) and mass of beetroot (0.5–1.5 g) using water as solvent. The data obtained from the experiments were analyzed by Pareto analysis of variance. Further, the data was fitted to a second-order polynomial equation using multiple regression analysis. The optimal conditions based on both individual and combinations of all responses (extraction temperature – 60 °C, time – 84 min and mass – 1.5 g) were found out. At this optimum condition, the total betalamic acid, betaxanthin and betacyanin content were found to be 9.9 mg/100 g, 16.3 mg/100 g and 30.9 mg/100 g with desirability value of 0.999. The experimental values closely agreed with the corresponding predicted values. •Betalain was extracted from red beetroot.•Extraction temperature, time and mass of beetroot were the independent variables.•Box–Behnken design of experiments was used to derive optimum process parameters.•Second order polynomial regression models were developed.•Optimal conditions were deduced by Derringer's desired function methodology.
AbstractList The aim of the study was to investigate the influence of independent process variables like extraction temperature, time and mass of beetroot on the aqueous extraction of betalain from beetroot. The optimum conditions for the aqueous extraction of betalamic acid, betaxanthin and betacyanin from beetroot were performed using a three-factor and three-level Box–Behnken design under response surface methodology. The pigments were extracted from beetroot at temperature (40–70 °C), time (30–90 min) and mass of beetroot (0.5–1.5 g) using water as solvent. The data obtained from the experiments were analyzed by Pareto analysis of variance. Further, the data was fitted to a second-order polynomial equation using multiple regression analysis. The optimal conditions based on both individual and combinations of all responses (extraction temperature – 60 °C, time – 84 min and mass – 1.5 g) were found out. At this optimum condition, the total betalamic acid, betaxanthin and betacyanin content were found to be 9.9 mg/100 g, 16.3 mg/100 g and 30.9 mg/100 g with desirability value of 0.999. The experimental values closely agreed with the corresponding predicted values. •Betalain was extracted from red beetroot.•Extraction temperature, time and mass of beetroot were the independent variables.•Box–Behnken design of experiments was used to derive optimum process parameters.•Second order polynomial regression models were developed.•Optimal conditions were deduced by Derringer's desired function methodology.
Author Chandrasekar, V.
Swamy, Gabriela John
Sangamithra, A.
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  givenname: V.
  surname: Chandrasekar
  fullname: Chandrasekar, V.
  organization: Agricultural Engineering College & Research Institute, TamilNadu Agricultural University, Coimbatore, TamilNadu, India
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Keywords Beetroot
Natural colorants
Box–Behnken design
Response surface methodology
Aqueous extraction
Optimization
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SubjectTerms Aqueous extraction
Beetroot
Box–Behnken design
Natural colorants
Optimization
Response surface methodology
Title Response surface modeling and process optimization of aqueous extraction of natural pigments from Beta vulgaris using Box–Behnken design of experiments
URI https://dx.doi.org/10.1016/j.dyepig.2014.05.028
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