Isolation of Cultured Endothelial Progenitor Cells in vitro from PBMCs and CD133~+ Enriched Cells
Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPC...
Saved in:
| Published in | Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 1; pp. 18 - 24 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
Heidelberg
Huazhong University of Science and Technology
01.02.2010
Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-0733 1993-1352 |
| DOI | 10.1007/s11596-010-0104-6 |
Cover
| Abstract | Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPCs.PBMCs from healthy subjects were used either directly for cell culture or for CD133+ sorting.The two groups of cells were cultured in complete medium 199(M199)for 7 to 14 days and the phenotypes of EPCs were an... |
|---|---|
| AbstractList | R3; Two isolation methods for sorting of endothelial progenitor cells(EPCs): from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPCs.PBMCs from healthy subjects were used either directly for cell culture or for CD133+ sorting.The two groups of cells were cultured in complete medium 199(M199)for 7 to 14 days and the phenotypes of EPCs were analyzed by FACS.The proliferation of differentiated EPCs was studied by MTT assay,and the VEGF concentration was measured using an ELISA kit.ECM gel experiment and migration assay were performed in vivo.The results showed that PBMCs produced more colony-forming units(CFU)than CD133+enriched cells from the same volume of blood(P<0.01).From day 7 to 14,the two groups showed decreased expression of hematopoietic stem cell markers and increased level of endothelial markers,but CD144+cells in CD133+ group were less than in PBMCs group(P<0.01).PBMCs group secreted more VEGF than CD133+group on the day 7(P<0.01).As compared with CD133+ group,PBMCs group had more potent potential of proliferation and vascularization in vitro.It was concluded that CD133+sorted cells showed a lower capacity of differentiation,secretion,proliferation and vascularization in vitro,suggesting that CD133-negative cells may be a preferential way to get EPCs for clinical therapy. Summary Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133 + enriched cells were compared, by defining the cell morphology, phenotype, reproductive activities and function in vitro , to provide a reference for clinical application of EPCs. PBMCs from healthy subjects were used either directly for cell culture or for CD133 + sorting. The two groups of cells were cultured in complete medium 199 (M199) for 7 to 14 days and the phenotypes of EPCs were analyzed by FACS. The proliferation of differentiated EPCs was studied by MTT assay, and the VEGF concentration was measured using an ELISA kit. ECM gel experiment and migration assay were performed in vivo . The results showed that PBMCs produced more colony-forming units (CFU) than CD133 + enriched cells from the same volume of blood ( P <0.01). From day 7 to 14, the two groups showed decreased expression of hematopoietic stem cell markers and increased level of endothelial markers, but CD144 + cells in CD133 + group were less than in PBMCs group ( P <0.01). PBMCs group secreted more VEGF than CD133 + group on the day 7 ( P <0.01). As compared with CD133 + group, PBMCs group had more potent potential of proliferation and vascularization in vitro . It was concluded that CD133 + sorted cells showed a lower capacity of differentiation, secretion, proliferation and vascularization in vitro , suggesting that CD133-negative cells may be a preferential way to get EPCs for clinical therapy. Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133(+) enriched cells were compared, by defining the cell morphology, phenotype, reproductive activities and function in vitro, to provide a reference for clinical application of EPCs. PBMCs from healthy subjects were used either directly for cell culture or for CD133(+) sorting. The two groups of cells were cultured in complete medium 199 (M199) for 7 to 14 days and the phenotypes of EPCs were analyzed by FACS. The proliferation of differentiated EPCs was studied by MTT assay, and the VEGF concentration was measured using an ELISA kit. ECM gel experiment and migration assay were performed in vivo. The results showed that PBMCs produced more colony-forming units (CFU) than CD133(+) enriched cells from the same volume of blood (P<0.01). From day 7 to 14, the two groups showed decreased expression of hematopoietic stem cell markers and increased level of endothelial markers, but CD144(+) cells in CD133(+) group were less than in PBMCs group (P<0.01). PBMCs group secreted more VEGF than CD133(+) group on the day 7 (P<0.01). As compared with CD133(+) group, PBMCs group had more potent potential of proliferation and vascularization in vitro. It was concluded that CD133(+) sorted cells showed a lower capacity of differentiation, secretion, proliferation and vascularization in vitro, suggesting that CD133-negative cells may be a preferential way to get EPCs for clinical therapy.Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133(+) enriched cells were compared, by defining the cell morphology, phenotype, reproductive activities and function in vitro, to provide a reference for clinical application of EPCs. PBMCs from healthy subjects were used either directly for cell culture or for CD133(+) sorting. The two groups of cells were cultured in complete medium 199 (M199) for 7 to 14 days and the phenotypes of EPCs were analyzed by FACS. The proliferation of differentiated EPCs was studied by MTT assay, and the VEGF concentration was measured using an ELISA kit. ECM gel experiment and migration assay were performed in vivo. The results showed that PBMCs produced more colony-forming units (CFU) than CD133(+) enriched cells from the same volume of blood (P<0.01). From day 7 to 14, the two groups showed decreased expression of hematopoietic stem cell markers and increased level of endothelial markers, but CD144(+) cells in CD133(+) group were less than in PBMCs group (P<0.01). PBMCs group secreted more VEGF than CD133(+) group on the day 7 (P<0.01). As compared with CD133(+) group, PBMCs group had more potent potential of proliferation and vascularization in vitro. It was concluded that CD133(+) sorted cells showed a lower capacity of differentiation, secretion, proliferation and vascularization in vitro, suggesting that CD133-negative cells may be a preferential way to get EPCs for clinical therapy. Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133(+) enriched cells were compared, by defining the cell morphology, phenotype, reproductive activities and function in vitro, to provide a reference for clinical application of EPCs. PBMCs from healthy subjects were used either directly for cell culture or for CD133(+) sorting. The two groups of cells were cultured in complete medium 199 (M199) for 7 to 14 days and the phenotypes of EPCs were analyzed by FACS. The proliferation of differentiated EPCs was studied by MTT assay, and the VEGF concentration was measured using an ELISA kit. ECM gel experiment and migration assay were performed in vivo. The results showed that PBMCs produced more colony-forming units (CFU) than CD133(+) enriched cells from the same volume of blood (P<0.01). From day 7 to 14, the two groups showed decreased expression of hematopoietic stem cell markers and increased level of endothelial markers, but CD144(+) cells in CD133(+) group were less than in PBMCs group (P<0.01). PBMCs group secreted more VEGF than CD133(+) group on the day 7 (P<0.01). As compared with CD133(+) group, PBMCs group had more potent potential of proliferation and vascularization in vitro. It was concluded that CD133(+) sorted cells showed a lower capacity of differentiation, secretion, proliferation and vascularization in vitro, suggesting that CD133-negative cells may be a preferential way to get EPCs for clinical therapy. Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPCs.PBMCs from healthy subjects were used either directly for cell culture or for CD133+ sorting.The two groups of cells were cultured in complete medium 199(M199)for 7 to 14 days and the phenotypes of EPCs were an... |
| Author | 郑伟红 万亚峰 马小鹏 李兴睿 杨志芳 殷茜 易继林 |
| AuthorAffiliation | Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology |
| AuthorAffiliation_xml | – name: Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| Author_xml | – sequence: 1 fullname: 郑伟红 万亚峰 马小鹏 李兴睿 杨志芳 殷茜 易继林 |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20155450$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kU9vFSEUxYmpse3TD-DGsDBx0YwCw5-ZpR2rNqmxC10TBpj3eJ0HLTDabvrZy3Semrhowg0szu8ezr3H4MAHbwF4jdF7jJD4kDBmLa8QRnPRij8DR7ht6wrXjByUNxekQqKuD8FxSluEmOCEvgCHBGHGKENHQJ2nMKrsgodhgN005ilaA8-8CXljR6dGeBnD2nqXQ4SdHccEnYe_XI4BDjHs4OXpty5B5Q3sPuG6vj8pcHR6U7o8yl-C54Mak321v1fg5-ezH93X6uL7l_Pu40WlKWpyZQZi1dCY3ljEdduKRvS0pDINowYj1RDGDVNaGMYIM9xoSo1BvGltjxtt6hU4Wfr-Vn5Qfi23YYq-OMq8vbsyt7e9tCU3mg8t6neL-jqGm8mmLHcu6fJf5W2YkixDa2veluGtwJu9cup31sjr6HYq3sk_MywCvAh0DClFO_yVYCTnPcllT7I4z0UlL4z4j9EuP64hR-XGJ0mykKm4-LWN_3I-Bb3d222CX98UTvZKXw1utDOCCWOiaesHeviwqQ |
| CitedBy_id | crossref_primary_10_1007_s11596_010_0652_9 crossref_primary_10_1002_jctb_2723 crossref_primary_10_1007_s10518_018_0403_z crossref_primary_10_1007_s11596_011_0566_1 |
| Cites_doi | 10.1161/01.CIR.0000058702.69484.A0 10.1016/j.jacc.2004.08.014 10.1172/JCI8071 10.1182/blood-2002-03-0711 10.1016/j.yjmcc.2005.07.003 10.1080/14653240701247853 10.1056/NEJMoa022287 10.1080/14653240601034708 10.1089/ten.2006.12.331 10.1161/CIRCULATIONAHA.107.694778 10.1126/science.275.5302.964 10.1016/S0092-8674(03)00687-1 10.1016/S0140-6736(02)09670-8 10.1016/j.exphem.2005.03.004 10.1016/j.athoracsur.2007.12.006 10.1161/01.RES.0000105569.77539.21 10.1161/ATVBAHA.107.144972 10.1038/sj.bmt.1703792 10.1182/blood-2006-08-043471 10.1152/ajpheart.01166.2004 10.1073/pnas.070046397 10.1016/S0008-6363(03)00252-9 10.1093/rheumatology/ken407 10.1016/j.critrevonc.2008.06.009 10.1182/blood.V95.10.3106 10.1182/blood.V95.9.2813.009k20_2813_2820 10.1073/pnas.97.7.3422 |
| ClassificationCodes | R3 |
| ContentType | Journal Article |
| Copyright | Huazhong University of Science and Technology and Springer Berlin Heidelberg 2010 Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Huazhong University of Science and Technology and Springer Berlin Heidelberg 2010 – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.1007/s11596-010-0104-6 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| DocumentTitleAlternate | Isolation of Cultured Endothelial Progenitor Cells in vitro from PBMCs and CD133~+ Enriched Cells |
| EISSN | 1993-1352 |
| EndPage | 24 |
| ExternalDocumentID | tjykdxxb_e201001004 20155450 10_1007_s11596_010_0104_6 1001255789 |
| Genre | Journal Article |
| GroupedDBID | -5E -5G -BR -Y2 -~C .86 .VR 06C 06D 0R~ 0VY 1N0 29K 29~ 2B. 2C~ 2J2 2JN 2KG 2KM 2LR 2RA 2~H 30V 4.4 408 40D 40E 53G 5GY 5VS 6NX 8TC 8UJ 92F 92I 92L 95- 95. 95~ 96X AAAVM AABHQ AAJKR AANXM AARHV AARTL AAYIU AAYQN AAYTO ABFTV ABJOX ABKCH ABMNI ABNWP ABQBU ABTMW ACGFS ACHXU ACKNC ACOMO ACSNA ACUDM ADHIR ADINQ ADKPE ADURQ ADYFF ADZKW AEBTG AEGNC AEJHL AEKMD AEOHA AEPYU AETLH AEXYK AFWTZ AFZKB AGAYW AGDGC AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHYZX AIIXL AJBLW AJRNO ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP ARMRJ AZFZN B-. BA0 BGNMA CAG CCEZO CHBEP CIEJG COF CQIGP CS3 CSCUP CW9 D-I EBS EJD ESBYG FA0 FEDTE FNLPD FRRFC FWDCC G-Y G-Z GGCAI GGRSB GJIRD GQ6 GQ7 H13 HF~ HG6 HMJXF HRMNR HVGLF HZ~ IJ- IPNFZ IXD I~X I~Z J-C JBSCW JUIAU KOV M4Y MA- N2Q NDZJH NQJWS NU0 O9- O93 O9I O9J P9S PF0 QOR QOS R-E R89 R9I RIG ROL RPX RSV S.. S16 S1Z S27 S37 S3B SAP SCL SDH SHX SMD SNE SNX SOJ SPISZ SZ9 SZN T13 TCJ TGQ TSG TT1 TUC U2A U9L UG4 VC2 W48 W91 WK8 Z7U Z82 Z8V ZOVNA ~A9 ~WA ABJNI ABQSL DPUIP AAYXX ADHKG AGQPQ CITATION CGR CUY CVF ECM EIF NPM 7X8 4A8 93N PSX |
| ID | FETCH-LOGICAL-c408t-df2eaf8dbde06c99787b4010d854d10a8256d5ac7d5525d6dc44dd0689eb18cd3 |
| IEDL.DBID | U2A |
| ISSN | 1672-0733 |
| IngestDate | Thu May 29 04:06:47 EDT 2025 Thu Sep 04 14:46:41 EDT 2025 Mon Jul 21 05:59:15 EDT 2025 Tue Jul 01 00:21:49 EDT 2025 Thu Apr 24 22:49:59 EDT 2025 Fri Feb 21 02:37:19 EST 2025 Fri Nov 25 07:22:36 EST 2022 |
| IsPeerReviewed | false |
| IsScholarly | false |
| Issue | 1 |
| Keywords | cell culture endothelial progenitor cells MACS |
| Language | English |
| License | http://www.springer.com/tdm |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c408t-df2eaf8dbde06c99787b4010d854d10a8256d5ac7d5525d6dc44dd0689eb18cd3 |
| Notes | endothelial progenitor cells; cell culture; MACS 42-1679/R cell culture endothelial progenitor cells R329 MACS ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 20155450 |
| PQID | 733936907 |
| PQPubID | 23479 |
| PageCount | 7 |
| ParticipantIDs | wanfang_journals_tjykdxxb_e201001004 proquest_miscellaneous_733936907 pubmed_primary_20155450 crossref_primary_10_1007_s11596_010_0104_6 crossref_citationtrail_10_1007_s11596_010_0104_6 springer_journals_10_1007_s11596_010_0104_6 chongqing_backfile_1001255789 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2010-02-01 |
| PublicationDateYYYYMMDD | 2010-02-01 |
| PublicationDate_xml | – month: 02 year: 2010 text: 2010-02-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Heidelberg |
| PublicationPlace_xml | – name: Heidelberg – name: China |
| PublicationSubtitle | Medical Sciences |
| PublicationTitle | Journal of Huazhong University of Science and Technology. Medical sciences |
| PublicationTitleAbbrev | J. Huazhong Univ. Sci. Technol. [Med. Sci.] |
| PublicationTitleAlternate | Journal of Zuazhong University of Science and Technology: Medical Edition |
| PublicationTitle_FL | JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY(MEDICAL SCIENCE) |
| PublicationYear | 2010 |
| Publisher | Huazhong University of Science and Technology Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| Publisher_xml | – name: Huazhong University of Science and Technology – name: Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| References | Bitan, Shapira, Resnick (CR26) 2005; 33 Beltrami, Barlucchi, Torella (CR5) 2003; 114 Dome, Timar, Ladanyi (CR9) 2009; 69 George, Shmilovich, Deutsch (CR16) 2006; 12 Kuci, Wessels, Bühring (CR11) 2003; 101 Lin, Weisdorf, Solovey (CR13) 2000; 105 Hur, Yang, Yoon (CR17) 2007; 116 Gordon, Leimig, Mueller (CR18) 2003; 31 Kalka, Masuda, Takahashi (CR2) 2000; 97 Yoder, Mead, Prater (CR19) 2007; 109 Mieno, Clements, Boodhwani (CR6) 2008; 85 Schachinger, Assmus, Britten (CR4) 2004; 44 Gallacher, Murdoch, Wu (CR10) 2000; 95 Gehling, Ergun, Schumacher (CR12) 2000; 95 Eggermann, Kliche, Jarmy (CR15) 2003; 58 Asahara, Murohara, Sullivan (CR1) 1997; 275 Lu, Baudouin, Gillespie (CR21) 2007; 9 Timmermans, Van Hauwermeiren, De Smedt (CR20) 2007; 27 Tateishi-Yuyama, Matsubara, Murohara (CR3) 2002; 360 Cañizo, Lozano, González-Porras (CR25) 2007; 9 Hill, Zalos, Halcox (CR7) 2003; 348 Gulati, Jevremovic, Peterson (CR8) 2003; 93 Urbich, Aicher, Heeschen (CR23) 2005; 39 Nevskaya, Ananieva, Bykovskaia (CR24) 2009; 48 Rehman, Li, Orschell (CR14) 2003; 107 He, Peterson, Katusic (CR22) 2005; 289 F. Timmermans (104_CR20) 2007; 27 M.C. Cañizo (104_CR25) 2007; 9 S. Kuci (104_CR11) 2003; 101 U.M. Gehling (104_CR12) 2000; 95 J. George (104_CR16) 2006; 12 L. Gallacher (104_CR10) 2000; 95 C. Urbich (104_CR23) 2005; 39 Y. Lin (104_CR13) 2000; 105 J. Eggermann (104_CR15) 2003; 58 J. Rehman (104_CR14) 2003; 107 J. Hur (104_CR17) 2007; 116 A.P. Beltrami (104_CR5) 2003; 114 T. Nevskaya (104_CR24) 2009; 48 S. Mieno (104_CR6) 2008; 85 T. He (104_CR22) 2005; 289 X. Lu (104_CR21) 2007; 9 M. Bitan (104_CR26) 2005; 33 P.R. Gordon (104_CR18) 2003; 31 T. Asahara (104_CR1) 1997; 275 V. Schachinger (104_CR4) 2004; 44 E. Tateishi-Yuyama (104_CR3) 2002; 360 R. Gulati (104_CR8) 2003; 93 M.C. Yoder (104_CR19) 2007; 109 C. Kalka (104_CR2) 2000; 97 J.M. Hill (104_CR7) 2003; 348 B. Dome (104_CR9) 2009; 69 16548691 - Tissue Eng. 2006 Feb;12(2):331-5 12584367 - N Engl J Med. 2003 Feb 13;348(7):593-600 12393715 - Blood. 2003 Feb 1;101(3):869-76 12757882 - Cardiovasc Res. 2003 May 1;58(2):478-86 16199052 - J Mol Cell Cardiol. 2005 Nov;39(5):733-42 10619863 - J Clin Invest. 2000 Jan;105(1):71-7 17495235 - Arterioscler Thromb Vasc Biol. 2007 Jul;27(7):1572-9 10725398 - Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3422-7 17464761 - Cytotherapy. 2007;9(3):292-300 14605020 - Circ Res. 2003 Nov 28;93(11):1023-5 12621502 - Bone Marrow Transplant. 2003 Jan;31(1):17-22 17909106 - Circulation. 2007 Oct 9;116(15):1671-82 15911096 - Exp Hematol. 2005 Jun;33(6):713-8 12615796 - Circulation. 2003 Mar 4;107(8):1164-9 15489105 - J Am Coll Cardiol. 2004 Oct 19;44(8):1690-9 10807776 - Blood. 2000 May 15;95(10):3106-12 17053059 - Blood. 2007 Mar 1;109(5):1801-9 12241713 - Lancet. 2002 Aug 10;360(9331):427-35 15805227 - Am J Physiol Heart Circ Physiol. 2005 Aug;289(2):H968-72 10779426 - Blood. 2000 May 1;95(9):2813-20 19022831 - Rheumatology (Oxford). 2009 Jan;48(1):61-4 17354105 - Cytotherapy. 2007;9(1):99-102 9020076 - Science. 1997 Feb 14;275(5302):964-7 18768327 - Crit Rev Oncol Hematol. 2009 Feb;69(2):108-24 18355528 - Ann Thorac Surg. 2008 Apr;85(4):1361-6 14505575 - Cell. 2003 Sep 19;114(6):763-76 |
| References_xml | – volume: 107 start-page: 1164 issue: 8 year: 2003 end-page: 1169 ident: CR14 article-title: Peripheral blood ldendothelial progenitor cells” are derived from monocyte/macrophages and secrete angiogenic growth factors publication-title: Circulation doi: 10.1161/01.CIR.0000058702.69484.A0 – volume: 44 start-page: 1690 issue: 8 year: 2004 end-page: 1699 ident: CR4 article-title: Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction: final one-year results of the TOPCARE-AMI Trial publication-title: J Am Coll Cardiol doi: 10.1016/j.jacc.2004.08.014 – volume: 105 start-page: 71 issue: 1 year: 2000 end-page: 77 ident: CR13 article-title: Origins of circulating endothelial cells and endothelial outgrowth from blood publication-title: J Clin Invest doi: 10.1172/JCI8071 – volume: 101 start-page: 869 issue: 3 year: 2003 end-page: 876 ident: CR11 article-title: Identification of a novel class of human adherent CD34-stem cells that give rise to SCID-repopulating cells publication-title: Blood doi: 10.1182/blood-2002-03-0711 – volume: 39 start-page: 733 issue: 5 year: 2005 end-page: 742 ident: CR23 article-title: Soluble factors released by endothelial progenitor cells promote migration of endothelial cells and cardiac resident progenitor cells publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2005.07.003 – volume: 9 start-page: 292 issue: 3 year: 2007 end-page: 300 ident: CR21 article-title: A comparison of CFU-GM, BFU-E and endothelial progenitor cells using expansion of selected cord blood CD133(+) and CD34(+) cells publication-title: Cytotherapy doi: 10.1080/14653240701247853 – volume: 348 start-page: 593 issue: 7 year: 2003 end-page: 600 ident: CR7 article-title: Circulating endothelial progenitor cells, vascular function, and cardiovascular risk publication-title: N Engl J Med doi: 10.1056/NEJMoa022287 – volume: 9 start-page: 99 issue: 1 year: 2007 end-page: 102 ident: CR25 article-title: Peripheral endothelial progenitor cells (CD133 ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up publication-title: Cytotherapy doi: 10.1080/14653240601034708 – volume: 12 start-page: 331 issue: 2 year: 2006 end-page: 335 ident: CR16 article-title: Comparative analysis of methods for assessment of circulating endothelial progenitor cells publication-title: Tissue Eng doi: 10.1089/ten.2006.12.331 – volume: 116 start-page: 1671 issue: 15 year: 2007 end-page: 1682 ident: CR17 article-title: Identification of a novel role of T cells in postnatal vasculogenesis: characterization of endothelial progenitor cell colonies publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.107.694778 – volume: 275 start-page: 964 issue: 5302 year: 1997 end-page: 967 ident: CR1 article-title: Isolation of putative progenitor endothelial cells for angiogenesis publication-title: Science doi: 10.1126/science.275.5302.964 – volume: 114 start-page: 763 issue: 6 year: 2003 end-page: 776 ident: CR5 article-title: Adult cardiac stem cells are multipotent and support myocardial regeneration publication-title: Cell doi: 10.1016/S0092-8674(03)00687-1 – volume: 360 start-page: 427 issue: 9331 year: 2002 end-page: 435 ident: CR3 article-title: Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomized controlled trial publication-title: Lancet doi: 10.1016/S0140-6736(02)09670-8 – volume: 33 start-page: 713 issue: 6 year: 2005 end-page: 718 ident: CR26 article-title: Successful transplantation of haploidentically mismatched peripheral blood stem cells using CD133 -purified stem cells publication-title: Exp Hematol doi: 10.1016/j.exphem.2005.03.004 – volume: 85 start-page: 1361 issue: 4 year: 2008 end-page: 1366 ident: CR6 article-title: Characteristics and function of cryopreserved bone marrow-derived endothelial progenitor cells publication-title: Ann Thorac Surg doi: 10.1016/j.athoracsur.2007.12.006 – volume: 93 start-page: 1023 issue: 11 year: 2003 end-page: 1025 ident: CR8 article-title: Diverse origin and function of cells with endothelial phenotype obtained from adult human blood publication-title: Circ Res doi: 10.1161/01.RES.0000105569.77539.21 – volume: 27 start-page: 1572 issue: 7 year: 2007 end-page: 1579 ident: CR20 article-title: Endothelial outgrowth cells are not derived from CD133+ cells or CD45+ hematopoietic precursors publication-title: Arterioscler Thromb Vasc Biol doi: 10.1161/ATVBAHA.107.144972 – volume: 31 start-page: 17 issue: 1 year: 2003 end-page: 22 ident: CR18 article-title: Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells publication-title: Bone Marrow Transplant doi: 10.1038/sj.bmt.1703792 – volume: 109 start-page: 1 801 issue: 5 year: 2007 end-page: 1809 ident: CR19 article-title: Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals publication-title: Blood doi: 10.1182/blood-2006-08-043471 – volume: 289 start-page: H968 issue: 2 year: 2005 end-page: 972 ident: CR22 article-title: Paracrine mitogenic effect of human endothelial progenitor cells: role of interleukin-8 publication-title: Am J Physiol Heart Circ Physiol doi: 10.1152/ajpheart.01166.2004 – volume: 97 start-page: 3422 issue: 7 year: 2000 end-page: 3427 ident: CR2 article-title: Transplantation of expanded endothelial progenitor cells for therapeutic neovascularization publication-title: Proc Nat Acad Sci USA doi: 10.1073/pnas.070046397 – volume: 58 start-page: 478 issue: 2 year: 2003 end-page: 486 ident: CR15 article-title: Endothelial progenitor cell culture and differentiation : a methodological comparison using human umbilical cord blood publication-title: Cardiovasc Res doi: 10.1016/S0008-6363(03)00252-9 – volume: 48 start-page: 61 issue: 1 year: 2009 end-page: 64 ident: CR24 article-title: Autologous progenitor cell implantation as a novel therapeutic intervention for ischaemic digits in systemic sclerosis publication-title: Rheumatology (Oxford) doi: 10.1093/rheumatology/ken407 – volume: 95 start-page: 3106 issue: 10 year: 2000 end-page: 3112 ident: CR12 article-title: differentiation of endothelial cells from AC133-positive progenitor cells publication-title: Blood – volume: 95 start-page: 2813 issue: 9 year: 2000 end-page: 2820 ident: CR10 article-title: Isolation and characterization of human CD34(−)Lin(−) and CD34(+)L in(−) hematopoietic stem cells using cell surface markers AC133 and CD7 publication-title: Blood – volume: 69 start-page: 108 issue: 2 year: 2009 end-page: 124 ident: CR9 article-title: Circulating endothelial cells, bone marrow-derived endothelial progenitor cells and proangiogenic hematopoietic cells in cancer: From biology to therapy publication-title: Crit Rev Oncol Hematol doi: 10.1016/j.critrevonc.2008.06.009 – volume: 275 start-page: 964 issue: 5302 year: 1997 ident: 104_CR1 publication-title: Science doi: 10.1126/science.275.5302.964 – volume: 58 start-page: 478 issue: 2 year: 2003 ident: 104_CR15 publication-title: Cardiovasc Res doi: 10.1016/S0008-6363(03)00252-9 – volume: 9 start-page: 292 issue: 3 year: 2007 ident: 104_CR21 publication-title: Cytotherapy doi: 10.1080/14653240701247853 – volume: 39 start-page: 733 issue: 5 year: 2005 ident: 104_CR23 publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2005.07.003 – volume: 114 start-page: 763 issue: 6 year: 2003 ident: 104_CR5 publication-title: Cell doi: 10.1016/S0092-8674(03)00687-1 – volume: 95 start-page: 3106 issue: 10 year: 2000 ident: 104_CR12 publication-title: Blood doi: 10.1182/blood.V95.10.3106 – volume: 109 start-page: 1 801 issue: 5 year: 2007 ident: 104_CR19 publication-title: Blood doi: 10.1182/blood-2006-08-043471 – volume: 348 start-page: 593 issue: 7 year: 2003 ident: 104_CR7 publication-title: N Engl J Med doi: 10.1056/NEJMoa022287 – volume: 95 start-page: 2813 issue: 9 year: 2000 ident: 104_CR10 publication-title: Blood doi: 10.1182/blood.V95.9.2813.009k20_2813_2820 – volume: 360 start-page: 427 issue: 9331 year: 2002 ident: 104_CR3 publication-title: Lancet doi: 10.1016/S0140-6736(02)09670-8 – volume: 69 start-page: 108 issue: 2 year: 2009 ident: 104_CR9 publication-title: Crit Rev Oncol Hematol doi: 10.1016/j.critrevonc.2008.06.009 – volume: 93 start-page: 1023 issue: 11 year: 2003 ident: 104_CR8 publication-title: Circ Res doi: 10.1161/01.RES.0000105569.77539.21 – volume: 9 start-page: 99 issue: 1 year: 2007 ident: 104_CR25 publication-title: Cytotherapy doi: 10.1080/14653240601034708 – volume: 97 start-page: 3422 issue: 7 year: 2000 ident: 104_CR2 publication-title: Proc Nat Acad Sci USA doi: 10.1073/pnas.97.7.3422 – volume: 44 start-page: 1690 issue: 8 year: 2004 ident: 104_CR4 publication-title: J Am Coll Cardiol doi: 10.1016/j.jacc.2004.08.014 – volume: 31 start-page: 17 issue: 1 year: 2003 ident: 104_CR18 publication-title: Bone Marrow Transplant doi: 10.1038/sj.bmt.1703792 – volume: 85 start-page: 1361 issue: 4 year: 2008 ident: 104_CR6 publication-title: Ann Thorac Surg doi: 10.1016/j.athoracsur.2007.12.006 – volume: 12 start-page: 331 issue: 2 year: 2006 ident: 104_CR16 publication-title: Tissue Eng doi: 10.1089/ten.2006.12.331 – volume: 289 start-page: H968 issue: 2 year: 2005 ident: 104_CR22 publication-title: Am J Physiol Heart Circ Physiol doi: 10.1152/ajpheart.01166.2004 – volume: 101 start-page: 869 issue: 3 year: 2003 ident: 104_CR11 publication-title: Blood doi: 10.1182/blood-2002-03-0711 – volume: 116 start-page: 1671 issue: 15 year: 2007 ident: 104_CR17 publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.107.694778 – volume: 107 start-page: 1164 issue: 8 year: 2003 ident: 104_CR14 publication-title: Circulation doi: 10.1161/01.CIR.0000058702.69484.A0 – volume: 105 start-page: 71 issue: 1 year: 2000 ident: 104_CR13 publication-title: J Clin Invest doi: 10.1172/JCI8071 – volume: 27 start-page: 1572 issue: 7 year: 2007 ident: 104_CR20 publication-title: Arterioscler Thromb Vasc Biol doi: 10.1161/ATVBAHA.107.144972 – volume: 48 start-page: 61 issue: 1 year: 2009 ident: 104_CR24 publication-title: Rheumatology (Oxford) doi: 10.1093/rheumatology/ken407 – volume: 33 start-page: 713 issue: 6 year: 2005 ident: 104_CR26 publication-title: Exp Hematol doi: 10.1016/j.exphem.2005.03.004 – reference: 15911096 - Exp Hematol. 2005 Jun;33(6):713-8 – reference: 19022831 - Rheumatology (Oxford). 2009 Jan;48(1):61-4 – reference: 18355528 - Ann Thorac Surg. 2008 Apr;85(4):1361-6 – reference: 12757882 - Cardiovasc Res. 2003 May 1;58(2):478-86 – reference: 17495235 - Arterioscler Thromb Vasc Biol. 2007 Jul;27(7):1572-9 – reference: 10725398 - Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3422-7 – reference: 12621502 - Bone Marrow Transplant. 2003 Jan;31(1):17-22 – reference: 17909106 - Circulation. 2007 Oct 9;116(15):1671-82 – reference: 10779426 - Blood. 2000 May 1;95(9):2813-20 – reference: 15489105 - J Am Coll Cardiol. 2004 Oct 19;44(8):1690-9 – reference: 17464761 - Cytotherapy. 2007;9(3):292-300 – reference: 18768327 - Crit Rev Oncol Hematol. 2009 Feb;69(2):108-24 – reference: 15805227 - Am J Physiol Heart Circ Physiol. 2005 Aug;289(2):H968-72 – reference: 12584367 - N Engl J Med. 2003 Feb 13;348(7):593-600 – reference: 9020076 - Science. 1997 Feb 14;275(5302):964-7 – reference: 17053059 - Blood. 2007 Mar 1;109(5):1801-9 – reference: 14505575 - Cell. 2003 Sep 19;114(6):763-76 – reference: 16548691 - Tissue Eng. 2006 Feb;12(2):331-5 – reference: 10807776 - Blood. 2000 May 15;95(10):3106-12 – reference: 14605020 - Circ Res. 2003 Nov 28;93(11):1023-5 – reference: 10619863 - J Clin Invest. 2000 Jan;105(1):71-7 – reference: 12393715 - Blood. 2003 Feb 1;101(3):869-76 – reference: 17354105 - Cytotherapy. 2007;9(1):99-102 – reference: 12241713 - Lancet. 2002 Aug 10;360(9331):427-35 – reference: 16199052 - J Mol Cell Cardiol. 2005 Nov;39(5):733-42 – reference: 12615796 - Circulation. 2003 Mar 4;107(8):1164-9 |
| SSID | ssj0057624 |
| Score | 1.4837353 |
| Snippet | Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were... Summary Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133 + enriched cells... Two isolation methods for sorting of endothelial progenitor cells (EPCs): from peripheral blood mononuclear cells (PBMCs) and CD133(+) enriched cells were... R3; Two isolation methods for sorting of endothelial progenitor cells(EPCs): from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were... |
| SourceID | wanfang proquest pubmed crossref springer chongqing |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 18 |
| SubjectTerms | AC133 Antigen Antigens, CD - metabolism Cell Separation - methods Cells, Cultured Endothelial Cells - cytology Endothelial Cells - metabolism Glycoproteins - metabolism Hematopoietic Stem Cells - cytology Humans Leukocytes, Mononuclear - cytology Medicine Medicine & Public Health Peptides - metabolism Vascular Endothelial Growth Factor A - metabolism |
| Title | Isolation of Cultured Endothelial Progenitor Cells in vitro from PBMCs and CD133~+ Enriched Cells |
| URI | http://lib.cqvip.com/qk/85740A/20101/1001255789.html https://link.springer.com/article/10.1007/s11596-010-0104-6 https://www.ncbi.nlm.nih.gov/pubmed/20155450 https://www.proquest.com/docview/733936907 https://d.wanfangdata.com.cn/periodical/tjykdxxb-e201001004 |
| Volume | 30 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3dT9wwDI82kKa9INjnwUB54GmoUmiTXPp43PjadNMedhJ76tI4BQZK4VoQ_PfY_bgDbUKa1L4lTmU7iV3bPzO2bY2W4L2LjBIQycTnUY6KhAJJ49gThlYTPZ9810dT-fVEnXR13FWf7d6HJJuTelHshjcveb-USCVkpF-yZUVwUqjE03jUH79oP7edbPWQ0iqTpA9l_osEASqcleH0Gpd7ejH9ZW0-ipQ29T2hsOH00VV0sMpWOhuSj1qhr7EXPrxhryZdlPwt-32MCtVwnJcFb7E1PHAfgMqtLlHjOKVledrNM06_7it-HvjteT0rOdWb8B97k3HFbQA-_oLu5Q7OnVHOKLSj37Hpwf7P8VHUdVKInBSmjqCIvS0M5OCFdil6jsMcHSsBRknYFRbdRA3KuiEoFSvQ4KQEENqkeJQbB8l7thTK4D8ynhiTy0RYlRRWpk0laxHveqW0V7lzesA25yzFm9hdEL4UISOjJYWnQzpgomdz5joYcuqGcZktAJRJShl-Hr0yQ5Kf51OuWgyO5wbzXnYZ7hTiig2-vKky1AXqXiiGA_ahlemcWkyWo1RiwHZ6IWfdXq6eW2q704PF4PrP_QXc3eWZpzQDeuT6fxHdYK_bBAXKmPnElurZjd9Eu6fOt9jy6PDXt_2tRt8fAG2v9v8 |
| linkProvider | Springer Nature |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlR3LbtQw0IIiARdU3gst-NATVSQ3sb3OsV2ottCtOHSl3ozjcdrSyoFNisrfdyaP3SJQJaTkZo-jmfE8Mi_GtpzREkLwiVECEpmFIimQkZAgeZoG6qHVRs9nR3o6l59P1Elfx10P2e5DSLKV1KtiN9S85P1SIpWQib7PHlCUkTyuebo7iF-0n7tJtnpMaZVZNoQy_wWCGiqcVfH0Jx73p2L6y9q8FSlt63ti6eLpLVW0v86e9DYk3-2I_pTdC_EZezjro-TP2bcDZKgW47wqeddbIwAPEajc6hI5jlNaVqDbvOD0677m55H_Om8WFad6E_51bzapuYvAJx_RvdzGvQvKGYVu9Qs23_90PJkm_SSFxEthmgTKNLjSQAFBaJ-j5zgu0LESYJSEHeHQTdSgnB-DUqkCDV5KAKFNjqLceMhesrVYxfCa8cyYQmbCqax0Mm8rWct0Jyilgyq81yO2uUQpamJ_Qf2lqDMyWlIoHfIREwOare_bkNM0jEu7aqBMVLL4efRKiyA_LLf86Hpw3LWYD7SzeFMIKy6G6qq2yAs0vVCMR-xVR9MltJQsR6nEiG0PRLb9Xa7vOmqr54PV4ub77wu4vi5soDQDeuSb_wL6nj2aHs8O7eHB0Ze37HGXrEDZMxtsrVlchU20gZriXcvzN0le-E4 |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwEB61i4R66fuxbWl94FQUMIntzR7pwhZKF3EoEj2FxONQWOTAJlvR_vrObJJd-hBSVSm52c5jxp4ZzTffAKymsVHonA1iLTFQkcuCjBSJBNIPQ8ccWrPs-ejA7B6pj8f6uOlzWrZo9zYlWdc0MEuTrzYuMd9YFL6RFeZImEFVUgXmLiwpbiHRgaWtD1_2d9rDmLzpuq-t6THIMoraxObfFmF6ha-FP72ih_9qpv7wPW_kTWfVPj5P_ekNwzR8ACftJ9V4lPH6tMrW7Y_f2B7_45sfwv3GaRVbtZY9gjvOP4blUZOWfwIne6TBMxGLIhc1mYdD4TxyfdcFqbhgHJjj42MiOFdQijMvvp1Vk0JwgYs4fD8alCL1KAbbFM-u0dwJg1SxHv0UjoY7nwe7QdO6IbAkhyrAPHRpHmOGThrbp1C1l1EkJzHWCjdlSnGpQZ3aHmodajRolUKUJu6T7YgtRs-g4wvvXoCI4jhTkUx1lKeqPyudzcNNp7VxOrPWdGFlLjUy_XbMhFZMxUyuGx1H_S7IVpKJbXjPuf3GRbJgbOY_m9Dr8a0SWvLdfMplTfpx22DRqkdCW5P_SupdMS0TUjdulyh7XXheq818tZBdVaVlF9ZawSfN4VHe9qjVRtUWg6vz72O8vs4Sx7gGvtTLf1r0LSwfbg-TT3sH-6_gXg2OYLTOa-hUk6lbIZ-ryt40--onWsYd-g |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Isolation+of+Cultured+Endothelial+Progenitor+Cells+in+vitro+from+PBMCs+and+CD133%7E%2B+Enriched+Cells&rft.jtitle=Journal+of+Huazhong+University+of+Science+and+Technology.+Medical+sciences&rft.au=%E9%83%91%E4%BC%9F%E7%BA%A2+%E4%B8%87%E4%BA%9A%E5%B3%B0+%E9%A9%AC%E5%B0%8F%E9%B9%8F+%E6%9D%8E%E5%85%B4%E7%9D%BF+%E6%9D%A8%E5%BF%97%E8%8A%B3+%E6%AE%B7%E8%8C%9C+%E6%98%93%E7%BB%A7%E6%9E%97&rft.date=2010-02-01&rft.issn=1672-0733&rft.eissn=1993-1352&rft.issue=1&rft_id=info:doi/10.1007%2Fs11596-010-0104-6&rft.externalDocID=1001255789 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F85740A%2F85740A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Ftjykdxxb-e%2Ftjykdxxb-e.jpg |