A novel soluble β-1,3- d -glucan Salecan reduces adiposity and improves glucose tolerance in high-fat diet-fed mice

• Published in: British journal of nutrition Vol. 109; no. 2; pp. 254 - 262
• Main Authors: Zhang, Ying, Xia, Lin, Pang, Wenqiang, Wang, Tao, Chen, Peng, Zhu, Bin, Zhang, Jianfa
• Format: Journal Article
• Language: English
• Published: Cambridge Cambridge University Press 28.01.2013
• Subjects: absorption; Adiposity; Agrobacterium; Animals; Anti-Obesity Agents - administration & dosage; Anti-Obesity Agents - chemistry; Anti-Obesity Agents - therapeutic use; beta-Glucans - administration & dosage; beta-Glucans - chemistry; beta-Glucans - therapeutic use; bile; Biological and medical sciences; blood serum; Cell Size; cellulose; Diet, High-Fat - adverse effects; Dietary Fats - analysis; Dietary Fats - metabolism; Dietary Supplements; droplets; emulsifying; Emulsifying Agents - administration & dosage; Emulsifying Agents - chemistry; Emulsifying Agents - therapeutic use; emulsions; energy; fat body; feces; Feces - chemistry; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; glucose; Glucose Intolerance - etiology; Glucose Intolerance - prevention & control; glucose tolerance; high density lipoprotein cholesterol; high fat diet; Intestinal Absorption; Intra-Abdominal Fat - pathology; Lipid Metabolism; liver; Liver - metabolism; Liver - pathology; Mice; Mice, Inbred C57BL; obesity; Obesity - etiology; Obesity - metabolism; Obesity - pathology; Obesity - prevention & control; Organ Size; polymers; Solubility; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Weight Gain; Obesity; β-Glucans; Salecan; Rodentia; Nutrition disorder; Tolerance; Glucose; Feeding; Glucan; Glucose tolerance; Vertebrata; Mammalia; Adiposity; Diet; Mouse; Animal; High-fat diet; Fat; Nutritional status
• ISSN: 0007-1145; 1475-2662; 1475-2662
• DOI: 10.1017/S0007114512000980

Salecan is a recently identified water-soluble viscous extracellular β-1,3- d -glucan polysaccharide from an Agrobacterium species. It is a high-molecular-mass polymer (about 2 × 10 6 Da) and composed of a linear chain of glucosyl residues linked through a repeat unit of seven β-(1,3) and two α-(1,3) glucosidic bonds. In the present study, we examined the effects of dietary Salecan fed at 2 and 5 % in a high-fat diet (64 % energy) in C57BL/6J mice. After 6 weeks, mice fed 2 and 5 % Salecan had significantly lower body weight, fat mass and percentage of body fat mass compared with those fed a high-fat cellulose (control) diet. Both the Salecan groups significantly and dose-dependently improved glucose tolerance, with a 9 and 26 % reduction of glucose AUC, respectively. Liver and adipose tissue weights were also significantly decreased by the Salecan treatment. Supplementation with 5 % Salecan led to lower serum TAG, total cholesterol (TC) and HDL-cholesterol (52, 18 and 19 %, respectively) and lower hepatic TAG by 56 % and TC by 22 % compared with the high-fat cellulose control group. Dietary Salecan intake caused an obvious elevation of fat in the faeces. Supplementation with Salecan disturbed bile acid-promoted emulsification and reduced the size of emulsion droplets in vitro. These results indicate that Salecan decreases fat absorption, improves glucose tolerance and has biologically important, dose-related effects on reducing high-fat diet-induced obesity.