DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy
Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable s...
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| Published in | Journal of clinical medicine Vol. 9; no. 5; p. 1341 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
MDPI AG
04.05.2020
MDPI |
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| Online Access | Get full text |
| ISSN | 2077-0383 2077-0383 |
| DOI | 10.3390/jcm9051341 |
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| Abstract | Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615–0.776), p < 0.001 for total sDF; 0.718 (0.640–0.797), p < 0.001 for viable sDF; 0.760 (0.685–0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells. |
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| AbstractList | Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615-0.776), p < 0.001 for total sDF; 0.718 (0.640-0.797), p < 0.001 for viable sDF; 0.760 (0.685-0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells.Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615-0.776), p < 0.001 for total sDF; 0.718 (0.640-0.797), p < 0.001 for viable sDF; 0.760 (0.685-0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells. Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615–0.776), p < 0.001 for total sDF; 0.718 (0.640–0.797), p < 0.001 for viable sDF; 0.760 (0.685–0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells. Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615–0.776), p < 0.001 for total sDF; 0.718 (0.640–0.797), p < 0.001 for viable sDF; 0.760 (0.685–0.835), p < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells. Sperm DNA fragmentation (sDF) negatively affects reproduction and is traditionally detected in total sperm population including viable and non-viable spermatozoa. Here, we aimed at exploring the ability of DNA fragmentation to discriminate fertile and subfertile men when detected in viable (viable sDF), non-viable (non-viable sDF), and total spermatozoa (total sDF). We revealed sDF in 91 male partners of infertile couples and 71 fertile men (max 1 year from natural conception) with LiveTUNEL coupled to flow cytometry, able to reveal simultaneously DNA fragmentation and cell viability. We found that the three sDF parameters discriminated fertile and subfertile men with similar accuracy and independently from age and basal semen parameters: AUCs (area under the curves) (95% CI) were: 0.696 (0.615-0.776), < 0.001 for total sDF; 0.718 (0.640-0.797), < 0.001 for viable sDF; 0.760 (0.685-0.835), < 0.001 for non-viable sDF. We also found that total and non-viable but not viable sDF significantly correlated to age and semen quality. In conclusion, the three sDF parameters similarly discriminated fertile and subfertile men. Viable spermatozoa with DNA fragmentation are likely cells able to fertilize the oocyte but failing to properly support subsequent embryo development. Non-viable sDF could be a sign of a subtler damage extended beyond the non-viable cells. |
| Author | Borini, Andrea Lotti, Francesco Boni, Luca Maggi, Mario Azzari, Chiara Muratori, Monica Pellegrino, Giulia Tarozzi, Nicoletta Baldi, Elisabetta Mangone, Giusi |
| AuthorAffiliation | 1 Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, Center of Excellence DeNothe, University of Florence, 50139 Florence, Italy; giulia.pellegrino3004@gmail.com (G.P.); francesco.lotti@unifi.it (F.L.); mario.maggi@unifi.it (M.M.) 2 Pediatric Section, Department of Health Sciences, University of Florence and Anna Meyer Children’s University Hospital, 50139 Florence, Italy; giusi.mangone@meyer.it (G.M.); chiara.azzari@meyer.it (C.A.) 4 Clinical Trials Center, AOU Careggi, 50139 Florence, Italy; bonil@aou-careggi.toscana.it 5 Department of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, 50139 Florence, Italy; elisabetta.baldi@unifi.it 3 9.baby, Family and Fertility Center, 40125 Bologna, Italy; nicoletta.tarozzi@9puntobaby.it (N.T.); andrea.borini@9puntobaby.it (A.B.) |
| AuthorAffiliation_xml | – name: 1 Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, Center of Excellence DeNothe, University of Florence, 50139 Florence, Italy; giulia.pellegrino3004@gmail.com (G.P.); francesco.lotti@unifi.it (F.L.); mario.maggi@unifi.it (M.M.) – name: 5 Department of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, 50139 Florence, Italy; elisabetta.baldi@unifi.it – name: 2 Pediatric Section, Department of Health Sciences, University of Florence and Anna Meyer Children’s University Hospital, 50139 Florence, Italy; giusi.mangone@meyer.it (G.M.); chiara.azzari@meyer.it (C.A.) – name: 3 9.baby, Family and Fertility Center, 40125 Bologna, Italy; nicoletta.tarozzi@9puntobaby.it (N.T.); andrea.borini@9puntobaby.it (A.B.) – name: 4 Clinical Trials Center, AOU Careggi, 50139 Florence, Italy; bonil@aou-careggi.toscana.it |
| Author_xml | – sequence: 1 givenname: Monica surname: Muratori fullname: Muratori, Monica – sequence: 2 givenname: Giulia surname: Pellegrino fullname: Pellegrino, Giulia – sequence: 3 givenname: Giusi surname: Mangone fullname: Mangone, Giusi – sequence: 4 givenname: Chiara surname: Azzari fullname: Azzari, Chiara – sequence: 5 givenname: Francesco surname: Lotti fullname: Lotti, Francesco – sequence: 6 givenname: Nicoletta surname: Tarozzi fullname: Tarozzi, Nicoletta – sequence: 7 givenname: Luca surname: Boni fullname: Boni, Luca – sequence: 8 givenname: Andrea orcidid: 0000-0001-9049-0020 surname: Borini fullname: Borini, Andrea – sequence: 9 givenname: Mario surname: Maggi fullname: Maggi, Mario – sequence: 10 givenname: Elisabetta surname: Baldi fullname: Baldi, Elisabetta |
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| Keywords | sperm DNA fragmentation male infertility viable spermatozoa |
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| SubjectTerms | Clinical medicine Data analysis Fertility Infertility Lasers Morphology Motility Oxidative stress Pregnancy Sperm |
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| Title | DNA Fragmentation in Viable and Non-Viable Spermatozoa Discriminates Fertile and Subfertile Subjects with Similar Accuracy |
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