A Large-Insert Porcine Library with Sevenfold Genome Coverage: a Tool for Positional Cloning of Candidate Genes for Major Quantitative Traits

A porcine genomic bacterial artificial chromosome (BAC) library was constructed by cloning partial EcoRI-digested high-molecular-weight DNA from a Korean native boar into the EcoRI site of the pBACe3.6 vector. The library consists of about 165,000 clones with an average insert size of 125 kb, repres...

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Published inMolecules and cells Vol. 16; no. 1; pp. 113 - 116
Main Authors Jeon, J.T. (Gyeongsang national University, Jinju, Republic of Korea), Jeon, H.J (Genomics Division, Bioneer Corporation, Daejeon, Republic of Korea), Kim, T.H, Lee, K.T, Cheong, I.C, Park, Y.W. (Rural Development Adiministration, Suwon, Republic of Korea)
Format Journal Article
LanguageEnglish
Published United States 한국분자세포생물학회 31.08.2003
Subjects
Animals
Cloning, Molecular - methods
Genome
GENOMES
Genomic Library
Microsatellite Repeats
Molecular Sequence Data
Quantitative Trait Loci
Swine - genetics
생물학
Online AccessGet full text
ISSN1016-8478
0219-1032
0219-1032
DOI10.1016/S1016-8478(23)13774-5

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Summary:A porcine genomic bacterial artificial chromosome (BAC) library was constructed by cloning partial EcoRI-digested high-molecular-weight DNA from a Korean native boar into the EcoRI site of the pBACe3.6 vector. The library consists of about 165,000 clones with an average insert size of 125 kb, representing about seven genome equivalents of coverage.
Bibliography:F01
2004005932
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content type line 23
G704-000079.2003.16.1.021
ISSN:1016-8478
0219-1032
0219-1032
DOI:10.1016/S1016-8478(23)13774-5