Bioleaching of chalcopyrite by UV-induced mutagenized Acidiphilium cryptum and Acidithiobacillus ferrooxidans

• Published in: Transactions of Nonferrous Metals Society of China Vol. 20; no. 2; pp. 315 - 321
• Main Author: 徐爱玲 夏金兰 张帅 杨宇 聂珍嫒 邱冠周
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2010; Key Laboratory of Biometallurgy of Ministry of Education of China,School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
• Subjects: Acidiphilium; Acidiphilium cryptum; Acidithiobacillus ferrooxidans; bioleaching; chalcopyrite; UV-induced mutagenesis; 最佳时间; 氧化亚铁硫杆菌; 生物浸出; 突变株; 突变率; 紫外线; 黄铜矿; Acidithiobacillus ferrooxidans; bioleaching; Acidiphilium cryptum; UV-induced mutagenesis; chalcopyrite
• ISSN: 1003-6326
• DOI: 10.1016/S1003-6326(09)60140-0

The original strains Acidithiobacillusferrooxidans GF and Acidiphilium cryptum DXI-1 were isolated from the drainage of some caves riched in chalcopyrite in Dexing Mine in Jiangxi Province of China. The optimum temperature and pH for growth were 30 ℃ and 3.5 for Ac. cryptum DXI-1, and 30 ℃ and 2.0 for At. ferrooxidans GF, respectively. For Ac. cryptum DXI-1, the optimum UV radiating time was 60 s and the positive mutation rate was 22.5%. The growth curves show that Ac. cryptum after mutagenesis reached stationary phase within 60 h, which was 20 h earlier than the original strain. For At. ferrooxidans GF, the optimum mutation time was 60 s and the positive mutation rate was 35%. The most active UV-mutated strain At. ferrooxidans GF oxidized all the ferrous after 48 h. The bioleaching experiments showed that bioleaching with the mixture of UV-mutated strains of At. ferrooxidans GF and A c. cryptum DX1-1 （1：1） could extract 3.01 g/L of copper after 30 d, while the extracted copper was 2.63 g/L with the mixture of the original strains before UV-mutation. At the end of the bioleaching experiments, the proportion of the cell density in the cultures ofAc. cryptum DXI-1 andAt.ferrooxidans GF was approximately 1：5.