Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans

Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐...

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Published inCell proliferation Vol. 56; no. 11; p. e13477
Main Authors Zhu, Meng‐Chao, Hu, Wei, Lin, Lei, Yang, Qing‐Wen, Zhang, Lu, Xu, Jia‐Lin, Xu, Yi‐Tong, Liu, Jia‐Sheng, Zhang, Meng‐Di, Tong, Xiao‐Yu, Zhu, Kai‐Yi, Feng, Ke, Feng, Yi, Su, Jian‐Zhong, Huang, Xiu‐Feng, Li, Jin
Format Journal Article
LanguageEnglish
Published England John Wiley & Sons, Inc 01.11.2023
John Wiley and Sons Inc
Subjects
Antibodies
Cell differentiation
Cells
Cells (biology)
Differentiation (biology)
Epithelial cells
Epithelium
Gene sequencing
Genes
Genomics
Heterogeneity
Lenses
Localization
Original
Progenitor cells
Ribonucleic acid
RNA
Spatial discrimination
Three dimensional imaging
United States > US
Online AccessGet full text
ISSN0960-7722
1365-2184
1365-2184
DOI10.1111/cpr.13477

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Abstract Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single‐cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4 + cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
AbstractList Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4+ cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single‐cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4 + cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation. scRNA‐seq reveals three cell types in lens superficial tissue with two differentiation trajectories between them, and a cluster might be lens epithelial stem/progenitor cell.
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4+ cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4+ cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single‐cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4 + cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4 cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
Author Feng, Yi
Xu, Yi‐Tong
Liu, Jia‐Sheng
Huang, Xiu‐Feng
Xu, Jia‐Lin
Zhang, Meng‐Di
Zhu, Kai‐Yi
Lin, Lei
Zhang, Lu
Tong, Xiao‐Yu
Su, Jian‐Zhong
Zhu, Meng‐Chao
Hu, Wei
Feng, Ke
Li, Jin
Yang, Qing‐Wen
AuthorAffiliation 4 Zhejiang Provincial Clinical Research Center for Pediatric Disease The Second Affiliated Hospital of Wenzhou Medical University Wenzhou Zhejiang China
2 National Clinical Research Center for Ocular Diseases, Eye Hospital Wenzhou Medical University Wenzhou China
1 State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital Wenzhou Medical University Wenzhou China
3 Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine Fudan University Shanghai China
AuthorAffiliation_xml – name: 4 Zhejiang Provincial Clinical Research Center for Pediatric Disease The Second Affiliated Hospital of Wenzhou Medical University Wenzhou Zhejiang China
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Copyright 2023 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.
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Meng‐Chao Zhu, Wei Hu, and Lei Lin contributed equally to this study.
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Snippet Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens...
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens...
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StartPage e13477
SubjectTerms Antibodies
Cell differentiation
Cells
Cells (biology)
Differentiation (biology)
Epithelial cells
Epithelium
Gene sequencing
Genes
Genomics
Heterogeneity
Lenses
Localization
Original
Progenitor cells
Ribonucleic acid
RNA
Spatial discrimination
Three dimensional imaging
Title Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans
URI https://www.ncbi.nlm.nih.gov/pubmed/37057399
https://www.proquest.com/docview/2885523326
https://www.proquest.com/docview/2801978602
https://pubmed.ncbi.nlm.nih.gov/PMC10623935
Volume 56
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