DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus

•Gibson gene assembly method was used to generate infectious clone of fowl adenovirus 4 (FAdV-4).•Purification and one-step growth curve of the rescued FAdV-4 was studied.•DNA assembly simplified the construction of infectious clone of adenovirus when compared with traditional methods. Plasmid beari...

Full description

Saved in:

Bibliographic Details
Published in	Journal of virological methods Vol. 257; pp. 85 - 92
Main Authors	Zou, Xiao-Hui, Bi, Zhi-Xiang, Guo, Xiao-Juan, Zhang, Zun, Zhao, Yang, Wang, Min, Zhu, Ya-Lu, Jie, Hong-Ying, Yu, Yang, Hung, Tao, Lu, Zhuo-Zhuang
Format	Journal Article
Language	English
Published	Netherlands Elsevier B.V 01.07.2018
Subjects	Animals Aviadenovirus - genetics Aviadenovirus - growth & development Cell Line Chickens DNA assembly DNA, Viral - genetics Escherichia coli Escherichia coli - genetics Fowl adenovirus 4 Fowl aviadenovirus C genes homologous recombination Human mastadenovirus C Infectious clone One-Step growth curve Plasmids Purification Recombination, Genetic reverse genetics Reverse Genetics - methods terminal repeat sequences Transfection viruses Infectious clone Purification Fowl adenovirus 4 One-Step growth curve DNA assembly
Online Access	Get full text
ISSN	0166-0934 1879-0984 1879-0984
DOI	10.1016/j.jviromet.2018.04.001

Cover

Abstract	•Gibson gene assembly method was used to generate infectious clone of fowl adenovirus 4 (FAdV-4).•Purification and one-step growth curve of the rescued FAdV-4 was studied.•DNA assembly simplified the construction of infectious clone of adenovirus when compared with traditional methods. Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics.
AbstractList	Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics. •Gibson gene assembly method was used to generate infectious clone of fowl adenovirus 4 (FAdV-4).•Purification and one-step growth curve of the rescued FAdV-4 was studied.•DNA assembly simplified the construction of infectious clone of adenovirus when compared with traditional methods. Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics. Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics.Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics.
Author	Bi, Zhi-Xiang Yu, Yang Zou, Xiao-Hui Lu, Zhuo-Zhuang Zhao, Yang Wang, Min Zhu, Ya-Lu Guo, Xiao-Juan Hung, Tao Zhang, Zun Jie, Hong-Ying
Author_xml	– sequence: 1 givenname: Xiao-Hui surname: Zou fullname: Zou, Xiao-Hui organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China – sequence: 2 givenname: Zhi-Xiang surname: Bi fullname: Bi, Zhi-Xiang organization: National Veterinary Product Engineering Research Center, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China – sequence: 3 givenname: Xiao-Juan surname: Guo fullname: Guo, Xiao-Juan organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China – sequence: 4 givenname: Zun surname: Zhang fullname: Zhang, Zun organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China – sequence: 5 givenname: Yang surname: Zhao fullname: Zhao, Yang organization: National Veterinary Product Engineering Research Center, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China – sequence: 6 givenname: Min surname: Wang fullname: Wang, Min organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China – sequence: 7 givenname: Ya-Lu surname: Zhu fullname: Zhu, Ya-Lu organization: National Veterinary Product Engineering Research Center, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China – sequence: 8 givenname: Hong-Ying surname: Jie fullname: Jie, Hong-Ying organization: National Veterinary Product Engineering Research Center, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China – sequence: 9 givenname: Yang surname: Yu fullname: Yu, Yang email: yangyu1719223@163.com organization: National Veterinary Product Engineering Research Center, Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China – sequence: 10 givenname: Tao surname: Hung fullname: Hung, Tao organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China – sequence: 11 givenname: Zhuo-Zhuang surname: Lu fullname: Lu, Zhuo-Zhuang email: luzz@ivdc.chinacdc.cn organization: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/29703616$$D View this record in MEDLINE/PubMed
BookMark	eNqFkUtv3CAUhVGVqJmk_QsRy27GvYBtjNRFozR9SFGzaRddIYwvCiMbpmCnyr8P1mQ23cyKh75zOZxzSc5CDEjINYOKAWs_7qrdk09xwrniwLoK6gqAvSEb1km1BdXVZ2RTwLbsRX1BLnPeAUAjhXhLLriSIFrWbsifLz9vqMkZp358pjPax-D_Lkizn_ajdx4znR-R2hjynBY7-xhodNQHh-thydSOxdl65-K_kZoBQyzOlvyOnDszZnz_ul6R31_vft1-394_fPtxe3O_tTWweculaKXE3km3mmtQ9UPbCFTW8oZj1_dNZxpAyXvl-NAY0wFih-2gyi9cL67Ih8PcfYrFeZ715LPFcTQBiz_Ny1DGQfHuNAqC1yAViIJev6JLP-Gg98lPJj3rY3IF-HQAbIo5J3Ta-tms-czJ-FEz0GtReqePRem1KA21LkUVefuf_PjCSeHngxBLpk8ek87WY7A4-FQq0UP0p0a8ADIWspI
CitedBy_id	crossref_primary_10_3390_v15081657 crossref_primary_10_3390_v12020228 crossref_primary_10_3390_v15010204 crossref_primary_10_1128_spectrum_00470_22 crossref_primary_10_1016_j_engmic_2023_100097 crossref_primary_10_1016_j_jbiotec_2019_11_009 crossref_primary_10_3390_v12030301 crossref_primary_10_3390_v15020529 crossref_primary_10_1089_hum_2018_258 crossref_primary_10_1016_j_omto_2021_11_011 crossref_primary_10_3390_genes13112129 crossref_primary_10_1128_jvi_00284_23 crossref_primary_10_3390_ijms21176370 crossref_primary_10_3390_genes13122316 crossref_primary_10_1186_s13567_025_01496_x crossref_primary_10_35118_apjmbb_2024_032_4_Special__08 crossref_primary_10_3390_v14030546 crossref_primary_10_3389_fmicb_2022_1041338
Cites_doi	10.1016/0042-6822(71)90175-9 10.1111/j.1348-0421.2006.tb03829.x 10.1128/JVI.70.7.4805-4810.1996 10.1016/j.gendis.2017.04.001 10.1007/BF01555121 10.1016/0022-2836(67)90307-5 10.1007/s00705-012-1293-z 10.1038/nmeth.1318 10.1016/j.celrep.2017.05.008 10.1002/jgm.1284 10.1016/S0147-9571(01)00032-7 10.1128/JVI.73.2.1399-1410.1999 10.1016/0092-8674(79)90275-7 10.1073/pnas.95.5.2509 10.1017/S0016672314000172 10.1016/0378-1119(87)90251-4 10.1007/BF01315225 10.1128/MCB.4.2.302 10.1007/978-1-4939-6472-7_24 10.1073/pnas.72.2.447 10.14405/kjvr.2011.51.3.209 10.1038/emi.2016.115 10.1002/j.1460-2075.1984.tb02232.x 10.1007/978-1-4939-6869-5_3 10.1034/j.1399-0004.2000.580101.x 10.2174/156652311796150363 10.1128/JVI.75.11.5288-5301.2001 10.1016/j.jviromet.2011.05.010 10.3390/ijms18040686
ContentType	Journal Article
Copyright	2018 Elsevier B.V. Copyright © 2018 Elsevier B.V. All rights reserved.
Copyright_xml	– notice: 2018 Elsevier B.V. – notice: Copyright © 2018 Elsevier B.V. All rights reserved.
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 7S9 L.6
DOI	10.1016/j.jviromet.2018.04.001
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic AGRICOLA AGRICOLA - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic AGRICOLA AGRICOLA - Academic
DatabaseTitleList	AGRICOLA MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	1879-0984
EndPage	92
ExternalDocumentID	29703616 10_1016_j_jviromet_2018_04_001 S0166093418300090
Genre	Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	--- --K --M .GJ .~1 0R~ 1B1 1RT 1~. 1~5 29L 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8P~ 9JM AAAJQ AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABBQC ABFNM ABFRF ABJNI ABLVK ABMAC ABMZM ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD AEBSH AEFWE AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGEKW AGHFR AGUBO AGYEJ AHHHB AI. AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CJTIS CNWQP CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HEJ HMG HMK HMO HVGLF HZ~ IH2 IHE J1W K-O KOM L7B LCYCR LUGTX M29 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SAE SCC SDF SDG SDP SES SEW SIN SPCBC SSH SSI SSZ T5K VH1 WUQ XFK Y6R ZGI ~G- ~KM AAHBH AATTM AAXKI AAYWO AAYXX ABWVN ACIEU ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGCQF AGQPQ AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP CITATION CGR CUY CVF ECM EIF NPM 7X8 EFKBS 7S9 ACLOT L.6 ~HD
ID	FETCH-LOGICAL-c401t-273677ebf7f57335e9bd653e9cc252e8bb58a50e72b9f2d5aa80ee8e6d9297fb3
IEDL.DBID	AIKHN
ISSN	0166-0934 1879-0984
IngestDate	Sun Sep 28 05:13:27 EDT 2025 Fri Sep 05 09:50:03 EDT 2025 Thu Apr 03 07:10:54 EDT 2025 Tue Jul 01 04:29:06 EDT 2025 Thu Apr 24 23:03:10 EDT 2025 Fri Feb 23 02:34:16 EST 2024
IsPeerReviewed	true
IsScholarly	true
Keywords	Infectious clone Purification Fowl adenovirus 4 One-Step growth curve DNA assembly
Language	English
License	Copyright © 2018 Elsevier B.V. All rights reserved.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c401t-273677ebf7f57335e9bd653e9cc252e8bb58a50e72b9f2d5aa80ee8e6d9297fb3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
PMID	29703616
PQID	2032407903
PQPubID	23479
PageCount	8
ParticipantIDs	proquest_miscellaneous_2335120928 proquest_miscellaneous_2032407903 pubmed_primary_29703616 crossref_citationtrail_10_1016_j_jviromet_2018_04_001 crossref_primary_10_1016_j_jviromet_2018_04_001 elsevier_sciencedirect_doi_10_1016_j_jviromet_2018_04_001
ProviderPackageCode	CITATION AAYXX
PublicationCentury	2000
PublicationDate	July 2018 2018-07-00 20180701
PublicationDateYYYYMMDD	2018-07-01
PublicationDate_xml	– month: 07 year: 2018 text: July 2018
PublicationDecade	2010
PublicationPlace	Netherlands
PublicationPlace_xml	– name: Netherlands
PublicationTitle	Journal of virological methods
PublicationTitleAlternate	J Virol Methods
PublicationYear	2018
Publisher	Elsevier B.V
Publisher_xml	– name: Elsevier B.V
References	Gelderblom, Maichle-Lauppe (bib0040) 1982; 72 King, Adams, Carstens, Lefkowitz (bib0085) 2011 Liu, Wan, Gao, Li, Yang, Liu, Yao, Chen, Wang, Zhao (bib0105) 2016; 5 Hirt (bib0070) 1967; 26 Khare, Chen, Weaver, Barry (bib0080) 2011; 11 Corredor, Pei, Nagy (bib0020) 2017; 1581 Jones, Shenk (bib0075) 1979; 17 Parks (bib0125) 2000; 58 Balamurugan, Kataria, Kataria, Verma, Nanthakumar (bib0005) 2002; 25 Chen, Dong, Li, Guo, Wang, Liu, Lu, Hung (bib0015) 2012; 157 Michou, Lehrmann, Saltik, Cotten (bib0115) 1999; 73 Petek, Felluga, Zoletto, Bersani (bib0130) 1964; 14 Graham (bib0055) 1984; 3 Fukuda, Terashima, Koshikawa, Kanegae, Saito (bib0035) 2006; 50 Li, Yu, Wang, Shen (bib0100) 2016; 35 Gianni, Smotkin, Weinberg (bib0045) 1975; 72 Francois, Eterradossi, Delmas, Payet, Langlois (bib0030) 2001; 75 Laver, Younghusband, Wrigley (bib0090) 1971; 45 Chartier, Degryse, Gantzer, Dieterle, Pavirani, Mehtali (bib0010) 1996; 70 He, Zhou, da Costa, Yu, Kinzler, Vogelstein (bib0065) 1998; 95 Yonemoto, Weyman (bib0145) 2017; 1498 Hanahan, Gluzman (bib0060) 1984; 4 Fougeroux, Holst (bib0025) 2017; 18 Thapana, Sujiwattanarat, Srikulnath, Hirai, Koga (bib0140) 2014; 96 Zou, Xiao, Chen, Li, Song, Wang, Qu, Lu, Hung (bib0155) 2011; 175 Shinagawa, Iida, Matsuda, Tsukiyama, Sato (bib0135) 1987; 55 Lee, Bishop, Zhang, Yu, Farina, Yan, Zhao, Zheng, Shu, Wu, Lei, Li, Zhang, Yang, Wu, Wu, Ho, Athiviraham, Lee, Wolf, Reid, He (bib0095) 2017; 4 Zhang, Fu, Liu, Wang, Schiwon, Janz, Schaffarczyk, von der Goltz, Ehrke-Schulz, Dorner, Solanki, Boehme, Bergmann, Lieber, Lauber, Dahl, Petzold, Zhang, Stewart, Ehrhardt (bib0150) 2017; 19 Lu, Zou, Dong, Qu, Song, Wang, Guo, Hung (bib0110) 2009; 11 Park, Lim, Kim, Kim, Yeo (bib0120) 2011; 51 Gibson, Young, Chuang, Venter, Hutchison, Smith (bib0050) 2009; 6 Parks (10.1016/j.jviromet.2018.04.001_bib0125) 2000; 58 Francois (10.1016/j.jviromet.2018.04.001_bib0030) 2001; 75 Zhang (10.1016/j.jviromet.2018.04.001_bib0150) 2017; 19 Zou (10.1016/j.jviromet.2018.04.001_bib0155) 2011; 175 Jones (10.1016/j.jviromet.2018.04.001_bib0075) 1979; 17 Thapana (10.1016/j.jviromet.2018.04.001_bib0140) 2014; 96 Balamurugan (10.1016/j.jviromet.2018.04.001_bib0005) 2002; 25 Fougeroux (10.1016/j.jviromet.2018.04.001_bib0025) 2017; 18 Liu (10.1016/j.jviromet.2018.04.001_bib0105) 2016; 5 Shinagawa (10.1016/j.jviromet.2018.04.001_bib0135) 1987; 55 Li (10.1016/j.jviromet.2018.04.001_bib0100) 2016; 35 Park (10.1016/j.jviromet.2018.04.001_bib0120) 2011; 51 Gelderblom (10.1016/j.jviromet.2018.04.001_bib0040) 1982; 72 Yonemoto (10.1016/j.jviromet.2018.04.001_bib0145) 2017; 1498 Hanahan (10.1016/j.jviromet.2018.04.001_bib0060) 1984; 4 Graham (10.1016/j.jviromet.2018.04.001_bib0055) 1984; 3 Petek (10.1016/j.jviromet.2018.04.001_bib0130) 1964; 14 Hirt (10.1016/j.jviromet.2018.04.001_bib0070) 1967; 26 Lu (10.1016/j.jviromet.2018.04.001_bib0110) 2009; 11 Laver (10.1016/j.jviromet.2018.04.001_bib0090) 1971; 45 Fukuda (10.1016/j.jviromet.2018.04.001_bib0035) 2006; 50 Corredor (10.1016/j.jviromet.2018.04.001_bib0020) 2017; 1581 Gibson (10.1016/j.jviromet.2018.04.001_bib0050) 2009; 6 Chen (10.1016/j.jviromet.2018.04.001_bib0015) 2012; 157 Gianni (10.1016/j.jviromet.2018.04.001_bib0045) 1975; 72 King (10.1016/j.jviromet.2018.04.001_bib0085) 2011 Chartier (10.1016/j.jviromet.2018.04.001_bib0010) 1996; 70 He (10.1016/j.jviromet.2018.04.001_bib0065) 1998; 95 Khare (10.1016/j.jviromet.2018.04.001_bib0080) 2011; 11 Lee (10.1016/j.jviromet.2018.04.001_bib0095) 2017; 4 Michou (10.1016/j.jviromet.2018.04.001_bib0115) 1999; 73
References_xml	– volume: 96 start-page: e13 year: 2014 ident: bib0140 article-title: Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria publication-title: Genet. Res. – volume: 72 start-page: 289 year: 1982 end-page: 298 ident: bib0040 article-title: The fibers of fowl adenoviruses publication-title: Arch. Virol. – volume: 51 start-page: 209 year: 2011 end-page: 216 ident: bib0120 article-title: Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea publication-title: Korean J. Vet. Res. – volume: 17 start-page: 683 year: 1979 end-page: 689 ident: bib0075 article-title: Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells publication-title: Cell – volume: 14 start-page: 637 year: 1964 end-page: 649 ident: bib0130 article-title: Further studies on celo virus: its relationship to the adenovirus group publication-title: Archiv fur Die Gesamte Virusforschung – volume: 5 start-page: e117 year: 2016 ident: bib0105 article-title: Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China publication-title: Emerg. Microbes Infect. – year: 2011 ident: bib0085 article-title: Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses – volume: 4 start-page: 302 year: 1984 end-page: 309 ident: bib0060 article-title: Rescue of functional replication origins from embedded configurations in a plasmid carrying the adenovirus genome publication-title: Mol. Cell. Biol. – volume: 11 start-page: 128 year: 2009 end-page: 138 ident: bib0110 article-title: Novel recombinant adenovirus type 41 vector and its biological properties publication-title: J. Gene Med. – volume: 157 start-page: 1313 year: 2012 end-page: 1321 ident: bib0015 article-title: Construction of an infectious clone of human adenovirus type 41 publication-title: Arch. Virol. – volume: 19 start-page: 1698 year: 2017 end-page: 1709 ident: bib0150 article-title: An engineered virus library as a Resource for the spectrum-wide exploration of virus and vector diversity publication-title: Cell Rep. – volume: 50 start-page: 643 year: 2006 end-page: 654 ident: bib0035 article-title: Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends publication-title: Microbiol. Immunol. – volume: 45 start-page: 598 year: 1971 end-page: 614 ident: bib0090 article-title: Purification and properties of chick embryo lethal orphan virus (an avian adenovirus) publication-title: Virology – volume: 6 start-page: 343 year: 2009 end-page: 345 ident: bib0050 article-title: Enzymatic assembly of DNA molecules up to several hundred kilobases publication-title: Nat. Methods – volume: 3 start-page: 2917 year: 1984 end-page: 2922 ident: bib0055 article-title: Covalently closed circles of human adenovirus DNA are infectious publication-title: EMBO J. – volume: 95 start-page: 2509 year: 1998 end-page: 2514 ident: bib0065 article-title: A simplified system for generating recombinant adenoviruses publication-title: Proc. Natl. Acad. Sci. U. S. A. – volume: 75 start-page: 5288 year: 2001 end-page: 5301 ident: bib0030 article-title: Construction of avian adenovirus CELO recombinants in cosmids publication-title: J. Virol. – volume: 1498 start-page: 359 year: 2017 end-page: 366 ident: bib0145 article-title: Facile site-directed mutagenesis of large constructs using gibson isothermal DNA assembly publication-title: Methods Mol. Biol. – volume: 58 start-page: 1 year: 2000 end-page: 11 ident: bib0125 article-title: Improvements in adenoviral vector technology: overcoming barriers for gene therapy publication-title: Clin. Genet. – volume: 55 start-page: 85 year: 1987 end-page: 93 ident: bib0135 article-title: Phylogenetic relationships between adenoviruses as inferred from nucleotide sequences of inverted terminal repeats publication-title: Gene – volume: 35 start-page: 5 year: 2016 end-page: 8 ident: bib0100 article-title: Establishment and application of a PCR method to detect fowl adenovirus group I based on hexon gene [in chinese] publication-title: Poult. Husb. Dis. Control – volume: 72 start-page: 447 year: 1975 end-page: 451 ident: bib0045 article-title: Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus publication-title: Proc. Natl. Acad. Sci. U. S. A. – volume: 175 start-page: 188 year: 2011 end-page: 196 ident: bib0155 article-title: An improved HAdV-41 E1B55K-expressing 293 cell line for packaging fastidious adenovirus publication-title: J. Virol. Methods – volume: 73 start-page: 1399 year: 1999 end-page: 1410 ident: bib0115 article-title: Mutational analysis of the avian adenovirus CELO, which provides a basis for gene delivery vectors publication-title: J. Virol. – volume: 11 start-page: 241 year: 2011 end-page: 258 ident: bib0080 article-title: Advances and future challenges in adenoviral vector pharmacology and targeting publication-title: Curr. Gene Ther. – volume: 4 start-page: 43 year: 2017 end-page: 63 ident: bib0095 article-title: Adenovirus-Mediated Gene delivery: potential applications for Gene and cell-based therapies in the New era of personalized medicine publication-title: Genes Dis. – volume: 1581 start-page: 29 year: 2017 end-page: 54 ident: bib0020 article-title: Fowl adenovirus-based vaccine platform publication-title: Methods Mol. Biol. – volume: 70 start-page: 4805 year: 1996 end-page: 4810 ident: bib0010 article-title: Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli publication-title: J. Virol. – volume: 25 start-page: 139 year: 2002 end-page: 147 ident: bib0005 article-title: Characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken publication-title: Comp. Immunol. Microbiol. Infect. Dis. – volume: 18 year: 2017 ident: bib0025 article-title: Future prospects for the development of cost-effective adenovirus vaccines publication-title: Int. J. Mol. Sci. – volume: 26 start-page: 365 year: 1967 end-page: 369 ident: bib0070 article-title: Selective extraction of polyoma DNA from infected mouse cell cultures publication-title: J. Mol. Biol. – volume: 45 start-page: 598 year: 1971 ident: 10.1016/j.jviromet.2018.04.001_bib0090 article-title: Purification and properties of chick embryo lethal orphan virus (an avian adenovirus) publication-title: Virology doi: 10.1016/0042-6822(71)90175-9 – volume: 50 start-page: 643 year: 2006 ident: 10.1016/j.jviromet.2018.04.001_bib0035 article-title: Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends publication-title: Microbiol. Immunol. doi: 10.1111/j.1348-0421.2006.tb03829.x – volume: 70 start-page: 4805 year: 1996 ident: 10.1016/j.jviromet.2018.04.001_bib0010 article-title: Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli publication-title: J. Virol. doi: 10.1128/JVI.70.7.4805-4810.1996 – volume: 4 start-page: 43 year: 2017 ident: 10.1016/j.jviromet.2018.04.001_bib0095 article-title: Adenovirus-Mediated Gene delivery: potential applications for Gene and cell-based therapies in the New era of personalized medicine publication-title: Genes Dis. doi: 10.1016/j.gendis.2017.04.001 – volume: 14 start-page: 637 year: 1964 ident: 10.1016/j.jviromet.2018.04.001_bib0130 article-title: Further studies on celo virus: its relationship to the adenovirus group publication-title: Archiv fur Die Gesamte Virusforschung doi: 10.1007/BF01555121 – volume: 26 start-page: 365 year: 1967 ident: 10.1016/j.jviromet.2018.04.001_bib0070 article-title: Selective extraction of polyoma DNA from infected mouse cell cultures publication-title: J. Mol. Biol. doi: 10.1016/0022-2836(67)90307-5 – volume: 157 start-page: 1313 year: 2012 ident: 10.1016/j.jviromet.2018.04.001_bib0015 article-title: Construction of an infectious clone of human adenovirus type 41 publication-title: Arch. Virol. doi: 10.1007/s00705-012-1293-z – volume: 6 start-page: 343 year: 2009 ident: 10.1016/j.jviromet.2018.04.001_bib0050 article-title: Enzymatic assembly of DNA molecules up to several hundred kilobases publication-title: Nat. Methods doi: 10.1038/nmeth.1318 – year: 2011 ident: 10.1016/j.jviromet.2018.04.001_bib0085 – volume: 19 start-page: 1698 year: 2017 ident: 10.1016/j.jviromet.2018.04.001_bib0150 article-title: An engineered virus library as a Resource for the spectrum-wide exploration of virus and vector diversity publication-title: Cell Rep. doi: 10.1016/j.celrep.2017.05.008 – volume: 11 start-page: 128 year: 2009 ident: 10.1016/j.jviromet.2018.04.001_bib0110 article-title: Novel recombinant adenovirus type 41 vector and its biological properties publication-title: J. Gene Med. doi: 10.1002/jgm.1284 – volume: 25 start-page: 139 year: 2002 ident: 10.1016/j.jviromet.2018.04.001_bib0005 article-title: Characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken publication-title: Comp. Immunol. Microbiol. Infect. Dis. doi: 10.1016/S0147-9571(01)00032-7 – volume: 73 start-page: 1399 year: 1999 ident: 10.1016/j.jviromet.2018.04.001_bib0115 article-title: Mutational analysis of the avian adenovirus CELO, which provides a basis for gene delivery vectors publication-title: J. Virol. doi: 10.1128/JVI.73.2.1399-1410.1999 – volume: 17 start-page: 683 year: 1979 ident: 10.1016/j.jviromet.2018.04.001_bib0075 article-title: Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells publication-title: Cell doi: 10.1016/0092-8674(79)90275-7 – volume: 95 start-page: 2509 year: 1998 ident: 10.1016/j.jviromet.2018.04.001_bib0065 article-title: A simplified system for generating recombinant adenoviruses publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.95.5.2509 – volume: 96 start-page: e13 year: 2014 ident: 10.1016/j.jviromet.2018.04.001_bib0140 article-title: Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria publication-title: Genet. Res. doi: 10.1017/S0016672314000172 – volume: 55 start-page: 85 year: 1987 ident: 10.1016/j.jviromet.2018.04.001_bib0135 article-title: Phylogenetic relationships between adenoviruses as inferred from nucleotide sequences of inverted terminal repeats publication-title: Gene doi: 10.1016/0378-1119(87)90251-4 – volume: 72 start-page: 289 year: 1982 ident: 10.1016/j.jviromet.2018.04.001_bib0040 article-title: The fibers of fowl adenoviruses publication-title: Arch. Virol. doi: 10.1007/BF01315225 – volume: 4 start-page: 302 year: 1984 ident: 10.1016/j.jviromet.2018.04.001_bib0060 article-title: Rescue of functional replication origins from embedded configurations in a plasmid carrying the adenovirus genome publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.4.2.302 – volume: 35 start-page: 5 year: 2016 ident: 10.1016/j.jviromet.2018.04.001_bib0100 article-title: Establishment and application of a PCR method to detect fowl adenovirus group I based on hexon gene [in chinese] publication-title: Poult. Husb. Dis. Control – volume: 1498 start-page: 359 year: 2017 ident: 10.1016/j.jviromet.2018.04.001_bib0145 article-title: Facile site-directed mutagenesis of large constructs using gibson isothermal DNA assembly publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-6472-7_24 – volume: 72 start-page: 447 year: 1975 ident: 10.1016/j.jviromet.2018.04.001_bib0045 article-title: Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.72.2.447 – volume: 51 start-page: 209 year: 2011 ident: 10.1016/j.jviromet.2018.04.001_bib0120 article-title: Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea publication-title: Korean J. Vet. Res. doi: 10.14405/kjvr.2011.51.3.209 – volume: 5 start-page: e117 year: 2016 ident: 10.1016/j.jviromet.2018.04.001_bib0105 article-title: Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China publication-title: Emerg. Microbes Infect. doi: 10.1038/emi.2016.115 – volume: 3 start-page: 2917 year: 1984 ident: 10.1016/j.jviromet.2018.04.001_bib0055 article-title: Covalently closed circles of human adenovirus DNA are infectious publication-title: EMBO J. doi: 10.1002/j.1460-2075.1984.tb02232.x – volume: 1581 start-page: 29 year: 2017 ident: 10.1016/j.jviromet.2018.04.001_bib0020 article-title: Fowl adenovirus-based vaccine platform publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-6869-5_3 – volume: 58 start-page: 1 year: 2000 ident: 10.1016/j.jviromet.2018.04.001_bib0125 article-title: Improvements in adenoviral vector technology: overcoming barriers for gene therapy publication-title: Clin. Genet. doi: 10.1034/j.1399-0004.2000.580101.x – volume: 11 start-page: 241 year: 2011 ident: 10.1016/j.jviromet.2018.04.001_bib0080 article-title: Advances and future challenges in adenoviral vector pharmacology and targeting publication-title: Curr. Gene Ther. doi: 10.2174/156652311796150363 – volume: 75 start-page: 5288 year: 2001 ident: 10.1016/j.jviromet.2018.04.001_bib0030 article-title: Construction of avian adenovirus CELO recombinants in cosmids publication-title: J. Virol. doi: 10.1128/JVI.75.11.5288-5301.2001 – volume: 175 start-page: 188 year: 2011 ident: 10.1016/j.jviromet.2018.04.001_bib0155 article-title: An improved HAdV-41 E1B55K-expressing 293 cell line for packaging fastidious adenovirus publication-title: J. Virol. Methods doi: 10.1016/j.jviromet.2011.05.010 – volume: 18 year: 2017 ident: 10.1016/j.jviromet.2018.04.001_bib0025 article-title: Future prospects for the development of cost-effective adenovirus vaccines publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms18040686
SSID	ssj0005733
Score	2.3143868
Snippet	•Gibson gene assembly method was used to generate infectious clone of fowl adenovirus 4 (FAdV-4).•Purification and one-step growth curve of the rescued FAdV-4... Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene...
SourceID	proquest pubmed crossref elsevier
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	85
SubjectTerms	Animals Aviadenovirus - genetics Aviadenovirus - growth & development Cell Line Chickens DNA assembly DNA, Viral - genetics Escherichia coli Escherichia coli - genetics Fowl adenovirus 4 Fowl aviadenovirus C genes homologous recombination Human mastadenovirus C Infectious clone One-Step growth curve Plasmids Purification Recombination, Genetic reverse genetics Reverse Genetics - methods terminal repeat sequences Transfection viruses
Title	DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus
URI	https://dx.doi.org/10.1016/j.jviromet.2018.04.001 https://www.ncbi.nlm.nih.gov/pubmed/29703616 https://www.proquest.com/docview/2032407903 https://www.proquest.com/docview/2335120928
Volume	257
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEB60IngR39YXK3iNTdJsdvdYfFAt9uAD9bRkk11oqY3QFvHib3emSaqCj4OnkLBDNt_O7nxD5gFwhEYbWb1T3nQXRrGfekoo6yEXl0JJbgKfkpOvunH7Lrp84A9zcFLlwlBYZXn2F2f69LQunzRKNBvPvV7jBslKjP54hEpJTAH99oUQrb2swULrotPufkR6iKKjPI73SOBTonD_uE_pZE-WwioDOa16WvaH-cZG_cRBp7bofAWWSxLJWsU8V2HODtdgsWgr-boOj6fdFkNSbJ_M4JXNqrSyUY_Cxx36xgxpH0vzj-qxLHesisuajFg6yIeWnrn8ZcASPJty_IDJaAPuzs9uT9pe2ULBS9FxGlPiTSyENU44QoFbZbKYN61K05CHVhrDZcJ9K0KjXJjxJJG-tdLGGdIm4UxzE2pDfOM2MBml0gWREDJBFoICQgnrBM8S4aTKwjrwCjSdlvXFqc3FQFeBZH1dga0JbO1HFFFXh8ZM7rmosPGnhKrWRH_RFY1m4E_Zw2oRNW4k-juSDC0iq6mVPHq3ym_-MgYhpGTjUNZhq9CA2ZwRL2QDQbzzj9ntwhLdFeHAe1BDJbD7SHrG5gDmj9-Cg1K16dq5vu-8A1KIAxw
linkProvider	Elsevier
linkToHtml	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LSwMxEA6lInoR39ZnBK_rPrrZJEdRS9W2FxX0FDa7CbS03UIr4sXf7sw-qoLag9dshs1-mUy-YedByBlc2sDqrXTyUxhGXuJILo0DXFxwKZj2PUxO7vai9mN4-8SeauSyyoXBsMrS9hc2PbfW5YhboulO-n33HshKBP54CEqJTAH89qUQ2xyAUp-_f4nz4EU_eZjt4PQvacKD8wEmk40MBlX6Iq95WnaH-eGG-o2B5jdRa52slRSSXhSr3CA1M94ky0VTybct8nzVu6BAic1ID9_ovEYrnfYxeNyCZ0yB9NEk-6wdSzNLq6islylNhtnY4JjNXoc0BsuUwQe8TLfJY-v64bLtlA0UnATcphmm3UScG225RRSYkTqNWNPIJAlYYITWTMTMMzzQ0gYpi2PhGSNMlAJp4lY3d0h9DG_cI1SEibB-yLmIgYOAAJfcWM7SmFsh06BBWAWaSsrq4tjkYqiqMLKBqsBWCLbyQoynaxB3Ljcp6msslJDVnqhvmqLgElgoe1ptooJjhP9G4rEBZBU2kgffVnrNP-YAhJhqHIgG2S00YL5mwAu4gB_t_2N1J2Sl_dDtqM5N7-6ArOKTIjD4kNRBIcwR0J-ZPs7V-wNZUwI7
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=DNA+assembly+technique+simplifies+the+construction+of+infectious+clone+of+fowl+adenovirus&rft.jtitle=Journal+of+virological+methods&rft.au=Zou%2C+Xiao-Hui&rft.au=Bi%2C+Zhi-Xiang&rft.au=Guo%2C+Xiao-Juan&rft.au=Zhang%2C+Zun&rft.date=2018-07-01&rft.issn=0166-0934&rft.volume=257+p.85-92&rft.spage=85&rft.epage=92&rft_id=info:doi/10.1016%2Fj.jviromet.2018.04.001&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0166-0934&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0166-0934&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0166-0934&client=summon