SUPERCRITICAL EXTRACTION OF LYOPHILIZED STRAWBERRY ANTHOCYANINS WITH PULSED ELECTRIC FIELDS PRETREATMENT

• Published in: Química Nova Vol. 45; no. 6; pp. 728 - 733
• Main Authors: Marco A. Ávila-Hernández, César Pérez-Alonso, Juan Orozco-Villafuerte, Carlos E. Barrera-Díaz, Erik Alpizar-Reyes, Julian Cruz-Olivares
• Format: Journal Article
• Language: English
• Published: Sociedade Brasileira de Química 01.01.2022
• Subjects: anthocyanins; lyophilized strawberry; pulsed electric fields; supercritical carbon dioxide; supercritical extraction
• ISSN: 1678-7064; 0100-4042; 1678-7064
• DOI: 10.21577/0100-4042.20170849

The lyophilized strawberry anthocyanins were extracted using a supercritical extraction (SE) process. The effect of pulsed electric fields (PEF) as pretreatment and the influence of the addition of ethanol as a cosolvent on the percentage of extraction yield (EY) and the total anthocyanin concentration (TAC) were analyzed. The effect of PEF was evaluated at 0.5 and 1.0 kV/cm, while the effect of the cosolvent was studied in mixtures of supercritical carbon dioxide - ethanol (SCCO2 + ethanol) at 1.6 and 3.3% by weight. The best results (% EY = 0.506, TAC = 0.428 g /100 g of lyophilized strawberry) were obtained with a PEF pretreatment of 1.0 kV cm-1, 3.3%wt. ethanol at 200 bar and 333.15 K. The experimental results of solubility were suitably adjusted with the Kumar and Johnston model. The maximum solubility (0.114 g/100 g of solvent mixture) was obtained at 300 bar and 313.15 K.