GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo／reperfusion rat

• Published in: Journal of Zhejiang University. B. Science Vol. 6; no. 4; pp. 254 - 258
• Main Author: 刘建仁 丁美萍 魏尔清 罗建红 宋英 黄鉴政 葛求富 胡华 朱丽君
• Format: Journal Article
• Language: English
• Published: China Department of Neurology, Second Affiliated Hospital, School of Medicine,Zhejiang University, Hangzhou 310009, China%Department of Pharmacology,School of Medicine, Zhejiang University, Hangzhou 310009, China%Department of Neurobiology, School of Medicine, Zhejiang University, Hangzhou 310009, China%Department of Neurosurgery,Second Affiliated Hospital, School of Medicine,Zhejiang University, Hangzhou 310009, China 01.04.2005; Zhejiang University Press
• Subjects: Animals; Biomedicine; Brain Ischemia - metabolism; Brain Ischemia - pathology; Brain Ischemia - prevention & control; G(M1) Ganglioside - pharmacology; G(M1) Ganglioside - therapeutic use; Gene Expression Regulation - drug effects; Male; Middle Cerebral Artery - surgery; Neurons - drug effects; Neurons - physiology; Protein Subunits - metabolism; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate - metabolism; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; Treatment Outcome; 再灌注; 冬氨酸盐; 神经节苷脂; 脑动脉栓塞; G(M1) ganglioside; Rats; N-methyl-D-aspartate receptors; Reperfusion; Middle cerebral artery occlusion
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.2005.B0254

Objective: To determine the protective effect ofmonosialoganglionside (GM 1) and evaluate the influence ofGM 1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ischemia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured, GM 1 ( 10 mg/kg) was given ip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2 h (group C) after MCA occlusion (MCAo). Expression of NMDAR 1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM 1 administered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01 and P<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM 1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 rain or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression ofNMDAR1.