Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction

• Published in: Talanta (Oxford) Vol. 221; p. 121594
• Main Authors: Hrdlička, Vojtěch, Barek, Jiří, Navrátil, Tomáš
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: biomarkers; Biomarkers, Tumor; boron; Boron doped diamond electrode; Catecholamine metabolites; Chromatography, High Pressure Liquid; detection; Differential pulse voltammetry; electrodes; Hollow fiber; Homovanillic Acid; Humans; Liquid Phase Microextraction; neoplasms; Neuroblastoma biomarkers; polypropylenes; sodium phosphate; supported liquid membranes; urine; voltammetry; Neuroblastoma biomarkers; Homovanillic acid; Hollow fiber; Catecholamine metabolites; Boron doped diamond electrode; Liquid phase microextraction; Differential pulse voltammetry
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2020.121594

Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5). [Display omitted] •New hollow-fiber microextraction determination of homovanillic acid.•Microextraction coupled with differential pulse voltammetry.•Cathodically pre-treated boron doped diamond working electrode.•Green solvent butyl benzoate as supported liquid membrane.•Method verified using real human urine sample.