Reference genes identification for qRT-PCR normalization of gene expression analysis in Cucumis sativus under Meloidogyne incognita infection and Pseudomonas treatment
qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide a...
Saved in:
| Published in | Frontiers in plant science Vol. 13; p. 1061921 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
Frontiers Media S.A
15.12.2022
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1664-462X 1664-462X |
| DOI | 10.3389/fpls.2022.1061921 |
Cover
| Abstract | qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from
M. incognita
. Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under
M. incognita
stress and
Pseudomonas
treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified
EF1
and
UBI
are the most suitable reference gene in the root knot and whole root of cucumber infected
M. incognita
, respectively, and
CACS
is the most suitable reference gene in the whole root of cucumber treated by
Pseudomonas
. The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or
Pseudomonas
inoculated, and these results would facilitate the further research on
M. incognita
or
Pseudomonas
soil rhizosphere microbe interaction with cucumber. |
|---|---|
| AbstractList | qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from M. incognita. Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under M. incognita stress and Pseudomonas treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified EF1 and UBI are the most suitable reference gene in the root knot and whole root of cucumber infected M. incognita, respectively, and CACS is the most suitable reference gene in the whole root of cucumber treated by Pseudomonas. The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or Pseudomonas inoculated, and these results would facilitate the further research on M. incognita or Pseudomonas soil rhizosphere microbe interaction with cucumber. qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from . Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under stress and treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified and are the most suitable reference gene in the root knot and whole root of cucumber infected , respectively, and is the most suitable reference gene in the whole root of cucumber treated by . The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or inoculated, and these results would facilitate the further research on or soil rhizosphere microbe interaction with cucumber. qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from M. incognita. Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under M. incognita stress and Pseudomonas treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified EF1 and UBI are the most suitable reference gene in the root knot and whole root of cucumber infected M. incognita, respectively, and CACS is the most suitable reference gene in the whole root of cucumber treated by Pseudomonas. The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or Pseudomonas inoculated, and these results would facilitate the further research on M. incognita or Pseudomonas soil rhizosphere microbe interaction with cucumber.qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from M. incognita. Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under M. incognita stress and Pseudomonas treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified EF1 and UBI are the most suitable reference gene in the root knot and whole root of cucumber infected M. incognita, respectively, and CACS is the most suitable reference gene in the whole root of cucumber treated by Pseudomonas. The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or Pseudomonas inoculated, and these results would facilitate the further research on M. incognita or Pseudomonas soil rhizosphere microbe interaction with cucumber. qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes. Cucumber as an economical vegetable is widely cultivated worldwide and is subject to serious nematode infection, especially from M. incognita . Plant could employ beneficial soil bacteria in the rhizosphere to enhance plant adaptability to various stresses. In this study, the optimal reference genes in cucumber under M. incognita stress and Pseudomonas treatment were calculated and confirmed. A total of thirteen candidate reference genes were identified across three different treatments. Of these, geNorm, NormFinder and BestKeeper programs combined RefFinder software identified EF1 and UBI are the most suitable reference gene in the root knot and whole root of cucumber infected M. incognita , respectively, and CACS is the most suitable reference gene in the whole root of cucumber treated by Pseudomonas . The work first validated the most suitable reference genes for the normalization gene expression in cucumber by nematode infected or Pseudomonas inoculated, and these results would facilitate the further research on M. incognita or Pseudomonas soil rhizosphere microbe interaction with cucumber. |
| Author | Liang, Meiting Tian, Yongqiang Ji, Tingting Wang, Xueyun Ma, Si Gao, Lihong |
| AuthorAffiliation | Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Horticulture, China Agriculture University , Beijing , China |
| AuthorAffiliation_xml | – name: Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops, College of Horticulture, China Agriculture University , Beijing , China |
| Author_xml | – sequence: 1 givenname: Tingting surname: Ji fullname: Ji, Tingting – sequence: 2 givenname: Si surname: Ma fullname: Ma, Si – sequence: 3 givenname: Meiting surname: Liang fullname: Liang, Meiting – sequence: 4 givenname: Xueyun surname: Wang fullname: Wang, Xueyun – sequence: 5 givenname: Lihong surname: Gao fullname: Gao, Lihong – sequence: 6 givenname: Yongqiang surname: Tian fullname: Tian, Yongqiang |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36589116$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNUsFu1DAQjVARLaUfwAXlyGUX23Hs-IKEVgUqFVGtisTNcpzx4sqxt3ZS2P5QfxOnWaqWA8IXj8fz3pvx88viwAcPRfEao2VVNeKd2bq0JIiQJUYMC4KfFUeYMbqgjHw_eBQfFicpXaG8aoSE4C-Kw4rVjcCYHRV3azAQwWsoN-AhlbYDP1hjtRps8KUJsbxeXy4uVuvSh9grZ2_nm2DuESX82kZIaUopr9wu2Uziy9Woxz6HKVffjKkcfQex_AIu2C5sdhlovQ4bbweVIwN6mBm68iLB2IU-eJXKIYIa-tzRq-K5US7ByX4_Lr59PL1cfV6cf_10tvpwvtCVqIdFQ2mFtSIINwigQwh0W9eoETWlLVKKIs4wJxpV-aC5MAQbpAhHFWcdFqg6Ls5m3i6oK7mNtldxJ4Oy8j4R4kaqOFjtQBKuQLQt41mCMkFbXTWkAUoU7VpSm8xFZq7Rb9Xup3LugRAjOZkoJxPlZKLcm5hB72fQdmx76HSePSr3pJOnN97-kJtwIwXP3pJpgrd7ghiuR0iDzDZocE55CGNW4wzlJ-CU5dI3j7UeRP58j1yA5wIdQ0oRzH8NwP_C6Ozx5G5u17p_IH8DDprlbw |
| CitedBy_id | crossref_primary_10_1038_s41467_024_51073_7 crossref_primary_10_3390_ijms26052133 crossref_primary_10_1016_j_plaphy_2024_109356 crossref_primary_10_1021_acs_jafc_4c08697 crossref_primary_10_1016_j_scienta_2023_112301 crossref_primary_10_1016_j_scienta_2023_112507 crossref_primary_10_1038_s41598_024_52948_x crossref_primary_10_1111_tpj_70094 crossref_primary_10_3389_fmicb_2024_1485167 crossref_primary_10_1038_s41598_024_73444_2 |
| Cites_doi | 10.1094/MPMI-23-8-1097 10.1094/MPMI-18-1247 10.1016/j.febslet.2007.12.039 10.1007/s12038-017-9728-z 10.1007/s11103-017-0621-9 10.1038/srep16961 10.1371/journal.pone.0072887 10.1038/nprot.2006.236 10.1104/pp.16.01051 10.1093/aobpla/plaa029 10.1146/annurev-phyto-080508-081846 10.1093/plcell/koaa018 10.1105/tpc.18.00905 10.1007/s10725-010-9479-4 10.4314/ACSJ.V20I1 10.1186/s12870-019-1629-x 10.1023/B:BILE.0000019559.84305.47 10.1016/j.envexpbot.2016.10.008 10.1093/pcp/pcq109 10.3390/ijms21176378 10.1073/pnas.1907968116 10.1093/hr/uhac197 10.1016/j.ab.2010.07.020 10.33804/pp.006.02.4207 10.1186/s12870-019-1953-1 10.1111/tpj.14224 10.1186/s12870-019-1971-z 10.1186/gb-2002-3-7-research0034 10.1104/pp.109.150557 10.1371/journal.pone.0066582 10.1186/s12864-015-1621-2 10.1007/s12041-017-0758-y 10.1038/protex.2017.107 10.1093/jxb/ery439 10.1007/978-981-13-8335-9 10.3390/ijms21030848 10.1105/tpc.18.00570 10.1111/jam.15481 10.1007/978-94-007-0434-3_10 10.3390/molecules21050573 10.1038/ng.475 10.1104/pp.15.00596 10.1093/nar/gkr954 10.1186/s12870-021-02900-2 10.1007/s00425-008-0706-4 10.1242/dev.180166 10.1111/mpp.12547 10.1093/jxb/eri285 10.3389/fpls.2017.01605 10.1371/annotation/6a5108f5-50f8-418e-854d-8f3eb94e6fc0 10.1104/pp.19.00317 10.1158/0008-5472.CAN-04-0496 10.1016/j.scienta.2019.109070 10.1371/journal.pone.0194436 10.1007/s11032-010-9487-0 10.7717/peerj.6536 10.1038/s41396-021-00974-2 10.1093/jxb/ery329 10.1007/s11105-015-0956-9 |
| ContentType | Journal Article |
| Copyright | Copyright © 2022 Ji, Ma, Liang, Wang, Gao and Tian. Copyright © 2022 Ji, Ma, Liang, Wang, Gao and Tian 2022 Ji, Ma, Liang, Wang, Gao and Tian |
| Copyright_xml | – notice: Copyright © 2022 Ji, Ma, Liang, Wang, Gao and Tian. – notice: Copyright © 2022 Ji, Ma, Liang, Wang, Gao and Tian 2022 Ji, Ma, Liang, Wang, Gao and Tian |
| DBID | AAYXX CITATION NPM 7X8 5PM ADTOC UNPAY DOA |
| DOI | 10.3389/fpls.2022.1061921 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) Unpaywall for CDI: Periodical Content Unpaywall DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic CrossRef |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: UNPAY name: Unpaywall url: https://proxy.k.utb.cz/login?url=https://unpaywall.org/ sourceTypes: Open Access Repository |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Botany |
| EISSN | 1664-462X |
| ExternalDocumentID | oai_doaj_org_article_27ae9bb67cb54694bc3828e42a4db25f 10.3389/fpls.2022.1061921 PMC9799720 36589116 10_3389_fpls_2022_1061921 |
| Genre | Journal Article |
| GroupedDBID | 5VS 9T4 AAFWJ AAKDD AAYXX ACGFO ACGFS ADBBV ADRAZ AENEX AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BCNDV CITATION EBD ECGQY GROUPED_DOAJ GX1 HYE KQ8 M48 M~E OK1 PGMZT RNS RPM ACXDI IAO IEA IGS IPNFZ ISR NPM RIG 7X8 5PM ADTOC UNPAY |
| ID | FETCH-LOGICAL-c395t-84431ca20180eed00ecb55089544b0aa4076172c030aac79f21f0a270376d1903 |
| IEDL.DBID | M48 |
| ISSN | 1664-462X |
| IngestDate | Fri Oct 03 12:51:15 EDT 2025 Sun Oct 26 03:54:35 EDT 2025 Tue Sep 30 17:17:27 EDT 2025 Fri Sep 05 08:35:21 EDT 2025 Thu Jan 02 22:54:00 EST 2025 Thu Apr 24 23:09:09 EDT 2025 Wed Oct 01 01:37:52 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | reference gene Cucumis sativus Meloidogyne incognita Pseudomonas qRT-PCR |
| Language | English |
| License | Copyright © 2022 Ji, Ma, Liang, Wang, Gao and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. cc-by |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c395t-84431ca20180eed00ecb55089544b0aa4076172c030aac79f21f0a270376d1903 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Erika V.S. Albuquerque, Brazilian Agricultural Research Corporation (EMBRAPA), Brazil; Tariq Mukhtar, Pir Mehr Ali Shah Arid Agriculture University, Pakistan These authors have contributed equally to this work Edited by: Carolina Escobar, University of Castilla-La Mancha, Spain This article was submitted to Plant Pathogen Interactions, a section of the journal Frontiers in Plant Science |
| OpenAccessLink | https://doaj.org/article/27ae9bb67cb54694bc3828e42a4db25f |
| PMID | 36589116 |
| PQID | 2760172746 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_27ae9bb67cb54694bc3828e42a4db25f unpaywall_primary_10_3389_fpls_2022_1061921 pubmedcentral_primary_oai_pubmedcentral_nih_gov_9799720 proquest_miscellaneous_2760172746 pubmed_primary_36589116 crossref_primary_10_3389_fpls_2022_1061921 crossref_citationtrail_10_3389_fpls_2022_1061921 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2022-12-15 |
| PublicationDateYYYYMMDD | 2022-12-15 |
| PublicationDate_xml | – month: 12 year: 2022 text: 2022-12-15 day: 15 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland |
| PublicationTitle | Frontiers in plant science |
| PublicationTitleAlternate | Front Plant Sci |
| PublicationYear | 2022 |
| Publisher | Frontiers Media S.A |
| Publisher_xml | – name: Frontiers Media S.A |
| References | Rajsz (B33) 2016; 34 Zhu (B59) 2009; 19 Catinot (B2) 2008; 582 Mbaluto (B22) 2020; 12 Kopczewski (B13) 2022; 21 Vejan (B44) 2016; 21 Le (B15) 2012; 7 Wen (B49) 2019; 146 Kumar (B14) 2019 Jones (B11) 2011 Yook (B51) 2011; 40 Rodrigues (B35) 2010; 406 Shahid (B37) 2022; 6 Li (B18) 2021; 15 Nicot (B26) 2005; 56 Zhang (B56) 2020; 262 Moens (B25) 2009; 47 Andersen (B1) 2004; 64 Vijayapalani (B45) 2018; 30 Zhao (B57) 2019; 31 Ma (B21) 2019; 98 Chen (B3) 2017; 134 Li (B17) 2013; 8 Hammes (B6) 2005; 18 Huang (B9) 2022; 9 Warzybok (B47) 2013; 8 Vandesompele (B43) 2002; 3 Jaiteh (B10) 2012; 20 Nolan (B28) 2006; 1 Qin (B32) 2021; 21 Migocka (B24) 2011; 28 Yuan (B52) 2022; 132 Olaetxea (B29) 2015; 169 Liang (B16) 2018; 13 Shukla (B39) 2018; 19 Uehara (B42) 2010; 51 Sandhya (B36) 2010; 62 Miao (B23) 2019; 7 Lü (B20) 2017; 95 Pfaffl (B31) 2004; 26 Xu (B50) 2019; 70 Joseph (B12) 2018; 43 Zhang (B55) 2019; 19 Song (B40) 2019; 19 Wei (B48) 2016; 172 Shen (B38) 2019; 116 Zhang (B53) 2021; 33 Hewezi (B7) 2010; 152 Duan (B4) 2017; 8 Huang (B8) 2009; 41 Zhou (B58) 2020; 21 Remans (B34) 2008; 227 Niu (B27) 2018; 69 Li (B19) 2017; 6159 Wang (B46) 2015; 16 Zhang (B54) 2010; 23 Gan (B5) 2017; 96 Petriccione (B30) 2015; 5 Sun (B41) 2019; 180 |
| References_xml | – volume: 23 start-page: 1097 year: 2010 ident: B54 article-title: Choline and osmotic-stress tolerance induced in arabidopsis by the soil microbe Bacillus subtilis (GB03) publication-title: Mol. Plant Microbe Interact. doi: 10.1094/MPMI-23-8-1097 – volume: 18 start-page: 1247 year: 2005 ident: B6 article-title: Nematode-induced changes of transporter gene expression in arabidopsis roots publication-title: Mol. Plant-Microbe Interact. doi: 10.1094/MPMI-18-1247 – volume: 582 start-page: 473 year: 2008 ident: B2 article-title: Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana publication-title: FEBS Lett. doi: 10.1016/j.febslet.2007.12.039 – volume: 43 start-page: 173 year: 2018 ident: B12 article-title: Plant reference genes for development and stress response studies publication-title: J. Biosci. doi: 10.1007/s12038-017-9728-z – volume: 95 start-page: 1 year: 2017 ident: B20 article-title: Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance publication-title: Plant Mol. Biol. doi: 10.1007/s11103-017-0621-9 – volume: 5 start-page: 1696 year: 2015 ident: B30 article-title: Reference gene selection for normalization of RT-qPCR gene expression data from actinidia deliciosa leaves infected with Pseudomonas syringae pv publication-title: Sci. Rep. doi: 10.1038/srep16961 – volume: 8 year: 2013 ident: B47 article-title: Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition publication-title: PloS One doi: 10.1371/journal.pone.0072887 – volume: 1 start-page: 1559 year: 2006 ident: B28 article-title: Quantification of mRNA using real-time RT- PCR publication-title: Nat. Protoc. doi: 10.1038/nprot.2006.236 – volume: 172 start-page: 603 year: 2016 ident: B48 article-title: Integrative analyses of nontargeted volatile profiling and transcriptome data provide molecular insight into VOC diversity in cucumber plants (Cucumis sativus) publication-title: Plant Physiol. doi: 10.1104/pp.16.01051 – volume: 12 start-page: plaa029 year: 2020 ident: B22 article-title: The impact of spodoptera exigua herbivory on Meloidogyne incognita induced root responses depends on the nematodeslife cycle stages publication-title: AoB Plants doi: 10.1093/aobpla/plaa029 – volume: 47 start-page: 313 year: 2009 ident: B25 article-title: Migratory plant endoparasitic nematodes: a group rich in contrasts and divergence publication-title: Annu. Rev. Phytopathol. doi: 10.1146/annurev-phyto-080508-081846 – volume: 33 start-page: 306 year: 2021 ident: B53 article-title: Gain-of-function of the 1-aminocyclopropane-1-carboxylate synthase gene ACS1G induces female flower development in cucumber gynoecy publication-title: Plant Cell. doi: 10.1093/plcell/koaa018 – volume: 31 start-page: 1289 year: 2019 ident: B57 article-title: A Functional Allele of CsFUL1 Regulates Fruit Length through Repressing CsSUP and Inhibiting Auxin Transport in Cucumber publication-title: Plant Cell doi: 10.1105/tpc.18.00905 – volume: 62 start-page: 21 year: 2010 ident: B36 article-title: Effect of plant growth promoting pseudomonas spp. 0n compatible solutes antioxidant status and plant growth of maize under drought stress publication-title: Plant Growth Regul. doi: 10.1007/s10725-010-9479-4 – volume: 20 start-page: 41 year: 2012 ident: B10 article-title: Evaluation of tomato genotypes for resistance to root-knot nematodes publication-title: Afr. Crop Sci. J. doi: 10.4314/ACSJ.V20I1 – volume: 19 start-page: 20 year: 2019 ident: B40 article-title: Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia rasiliens schw publication-title: BMC Plant Biol. doi: 10.1186/s12870-019-1629-x – volume: 26 start-page: 509 year: 2004 ident: B31 article-title: Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: best-keeper- excel-based tool using pair-wise correlations publication-title: Biotechnol. Lett. doi: 10.1023/B:BILE.0000019559.84305.47 – volume: 134 start-page: 1 year: 2017 ident: B3 article-title: Blueberry VcLON 2, a peroxisomal LON protease, is involved in abiotic stress tolerance publication-title: Environ. Exp. Bot. doi: 10.1016/j.envexpbot.2016.10.008 – volume: 51 start-page: 1524 year: 2010 ident: B42 article-title: Resistant and susceptible responses in tomato to cyst nematode are differentially regulated by salicylic acid publication-title: Plant Cell Physiol. doi: 10.1093/pcp/pcq109 – volume: 21 start-page: 6378 year: 2022 ident: B13 article-title: Local and systemic changes in photosynthetic parameters and antioxidant activity in cucumber challenged with Pseudomonas syringae pv lachrymans publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms21176378 – volume: 116 start-page: 17105 year: 2019 ident: B38 article-title: CsBRC1 inhibits axillary bud outgrowth by directly repressing the auxin efflux carrier CsPIN3 in cucumber publication-title: Proc. Natl. Acad. Sci. U S A. doi: 10.1073/pnas.1907968116 – volume: 9 start-page: uhac197 year: 2022 ident: B9 article-title: SlWRKY45 interacts with jasmonate-ZIM domain proteins to negatively regulate defense against the root-knot nematode Meloidogyne incognita in tomato publication-title: Horticult. Res. doi: 10.1093/hr/uhac197 – volume: 406 start-page: 185 year: 2010 ident: B35 article-title: The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean publication-title: Anal. Biochem. doi: 10.1016/j.ab.2010.07.020 – volume: 6 start-page: 133 year: 2022 ident: B37 article-title: Studies on the possible role of plant host on the development of root-knot nematode, Meloidogyne javanica and Pasteuria penetrans as affected by different harvesting dates publication-title: Plant Prot. doi: 10.33804/pp.006.02.4207 – volume: 19 start-page: 1 year: 2009 ident: B59 article-title: Genome-wide identification, structure characterization, and expression pattern profiling of aquaporin gene family in cucumber publication-title: BMC Plant Biol. doi: 10.1186/s12870-019-1953-1 – volume: 98 start-page: 391 year: 2019 ident: B21 article-title: Phloem loading in cucumber: combined symplastic andapoplastic strategies publication-title: Plant J. doi: 10.1111/tpj.14224 – volume: 19 start-page: 371 year: 2019 ident: B55 article-title: Expression and functional analysis of the propamocarb-related gene CsMAPEG in cucumber publication-title: BMC Plant Biol. doi: 10.1186/s12870-019-1971-z – volume: 3 start-page: 1 year: 2002 ident: B43 article-title: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes publication-title: Genome Biol. doi: 10.1186/gb-2002-3-7-research0034 – volume: 152 start-page: 968 year: 2010 ident: B7 article-title: Arabidopsis spermidine synthase is targeted by an effector protein of the cyst nematode Heterodera schachtii publication-title: Plant Physiol. doi: 10.1104/pp.109.150557 – volume: 8 start-page: 1 year: 2013 ident: B17 article-title: Brassinosteroids-induced systemic stress tolerance was associated with increased transcripts of several defence-related genes in the phloem in Cucumis sativus publication-title: PloS One doi: 10.1371/journal.pone.0066582 – volume: 16 start-page: 386 year: 2015 ident: B46 article-title: Genome-wide identification of MAPK, MAPKK, and MAPKKK gene families and transcriptional profiling analysis during development and stress response in cucumber publication-title: BMC Genomics doi: 10.1186/s12864-015-1621-2 – volume: 96 start-page: 235 year: 2017 ident: B5 article-title: Expression analysis of argonaute, dicer-like, and RNA-dependent RNA polymerase genes in cucumber (Cucumis sativus l.) in response to abiotic stress publication-title: J. Genet. doi: 10.1007/s12041-017-0758-y – volume: 6159 year: 2017 ident: B19 article-title: A protocol for agrobacterium-mediated transformation of cucumber (Cucumis sativus l.) from cotyledon explants publication-title: Protocol. exch doi: 10.1038/protex.2017.107 – volume: 70 start-page: 1325 year: 2019 ident: B50 article-title: Strigolactones positively regulate defense against root-knot nematodes in tomato publication-title: J. Exp. Bot. doi: 10.1093/jxb/ery439 – volume-title: Saline soil-based agriculture by halotolerant microorganisms year: 2019 ident: B14 doi: 10.1007/978-981-13-8335-9 – volume: 21 start-page: 848 year: 2020 ident: B58 article-title: Transcriptome analysis of rice roots in response to root-knot nematode infection publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms21030848 – volume: 30 start-page: 2795 year: 2018 ident: B45 article-title: An effector from the Cyst nematode heterodera schachtii derepresses host rRNA genes by altering histone acetylation publication-title: Plant Cell. doi: 10.1105/tpc.18.00570 – volume: 132 start-page: 3717 year: 2022 ident: B52 article-title: Selection and evaluation of suitable reference genes for quantitative gene expression analysis during infection of Cucumis sativus with Pectobacterium rasiliense publication-title: J. Appl. Microbiol. doi: 10.1111/jam.15481 – start-page: 175 volume-title: Genomics and molecular genetics of plant–nematode interactions year: 2011 ident: B11 article-title: C. elegans as a resource for studies on plant-parasitic nematodes doi: 10.1007/978-94-007-0434-3_10 – volume: 21 start-page: 573 year: 2016 ident: B44 article-title: Role of plant growth promoting rhizobacteria in agricultural sustainability-a review publication-title: Molecules doi: 10.3390/molecules21050573 – volume: 41 start-page: 1275 year: 2009 ident: B8 article-title: The genome of the cucumber, Cucumis sativus l publication-title: Nat. Genet. doi: 10.1038/ng.475 – volume: 169 start-page: 2587 year: 2015 ident: B29 article-title: Abscisic acid regulation of root hhydraulic conductivity and aquaporin gene expression is crucial to the plant shoot growth eenhancement caused by rhizosphere humic acids publication-title: Plant Physiol. doi: 10.1104/pp.15.00596 – volume: 40 start-page: 735 year: 2011 ident: B51 article-title: Wormbase 2012: more genomics, more data, new website publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkr954 – volume: 21 start-page: 143 year: 2021 ident: B32 article-title: Genome-wide identification of CLE gene family and their potential roles in bolting and fruit bearing in cucumber (Cucumis sativus l.) publication-title: BMC Plant Biol. doi: 10.1186/s12870-021-02900-2 – volume: 227 start-page: 1343 year: 2008 ident: B34 article-title: Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations publication-title: Planta doi: 10.1007/s00425-008-0706-4 – volume: 146 start-page: dev180166 year: 2019 ident: B49 article-title: CsTFL1 inhibits determinate growth and terminal flower formation through interaction with CsNOT2a in cucumber publication-title: Development doi: 10.1242/dev.180166 – volume: 19 start-page: 615 year: 2018 ident: B39 article-title: Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-infected tomato (Solanum lycopersicum) roots reveals complex gene expression profiles and metabolic networks of both host and nematode during susceptible and resistance responses publication-title: Mol. Plant Pathol. doi: 10.1111/mpp.12547 – volume: 56 start-page: 2907 year: 2005 ident: B26 article-title: Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress publication-title: J. Exp. Bot. doi: 10.1093/jxb/eri285 – volume: 8 year: 2017 ident: B4 article-title: Identification of optimal reference genes for expression analysis in radish (Raphanus sativus l.) and its relatives based on expression stability publication-title: Front. Plant Sci. doi: 10.3389/fpls.2017.01605 – volume: 7 year: 2012 ident: B15 article-title: Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions publication-title: PloS One doi: 10.1371/annotation/6a5108f5-50f8-418e-854d-8f3eb94e6fc0 – volume: 180 start-page: 986 year: 2019 ident: B41 article-title: Down-regulation of the sucrose transporter CsSUT1 causes male sterility by altering carbohydrate supply publication-title: Plant Physiol. doi: 10.1104/pp.19.00317 – volume: 64 start-page: 5245 year: 2004 ident: B1 article-title: Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-04-0496 – volume: 262 start-page: 109070 year: 2020 ident: B56 article-title: Alleviating effects of exogenous melatonin on salt stress in cucumber publication-title: Sci. Hortic. doi: 10.1016/j.scienta.2019.109070 – volume: 13 year: 2018 ident: B16 article-title: Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress publication-title: PloS One doi: 10.1371/journal.pone.0194436 – volume: 28 start-page: 343 year: 2011 ident: B24 article-title: Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators publication-title: Mol. Breed. doi: 10.1007/s11032-010-9487-0 – volume: 7 year: 2019 ident: B23 article-title: Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus l.), pumpkin (Cucurbita moschata duch.) and cucumber-pumpkin grafted plants publication-title: Peer J. doi: 10.7717/peerj.6536 – volume: 15 start-page: 2865 year: 2021 ident: B18 article-title: Salt-induced recruitment of specific root-associated bacterial consortium capable of enhancing plant adaptability to salt stress publication-title: Multidiscip. J. Microbial. Ecol. J. doi: 10.1038/s41396-021-00974-2 – volume: 69 start-page: 5373 year: 2018 ident: B27 article-title: The WUSCHEL-related homeobox1 gene of cucumber regulates reproductive organ development publication-title: J. Exp. Bot. doi: 10.1093/jxb/ery329 – volume: 34 start-page: 720 year: 2016 ident: B33 article-title: Genes encoding cucumber full-size ABCG proteins show different responses to plant growth regulators and sclareolide publication-title: Plant Mol. Biol. Rep. doi: 10.1007/s11105-015-0956-9 |
| SSID | ssj0000500997 |
| Score | 2.3993254 |
| Snippet | qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is... |
| SourceID | doaj unpaywall pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 1061921 |
| SubjectTerms | Cucumis sativus Meloidogyne incognita Plant Science Pseudomonas qRT-PCR reference gene |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1ba9RAGB2kFPRFrNeolRF8UmKTySSz89guliJUSmmhb2GudSFM1u7Gdn-Rf9PvS2bDLop98XWTye2cnZwvc-YMIR-8R_OgY2kmjUq5YzzVHlNEC8EYAO5sH7t4-q06ueRfr8qrjaW-0BM2xAMPD-6ACeWk1pUwuoRSjmtTQJHgOFPcalZ67H2zidwopoZUb5Q-YhjGhCpMHvh5g-ncjH3GIkiyfOtF1Of1_01k_umVfNiFuVrdqqbZeBEdPyGPo4Kkh8OV75EHLjwlu0ctqLzVM_JrTI6l19iN0ZmNfqAeAgoalf44v0jPpuc0oF5t4kRM2vq-BXV30RsbqIqJJXQW6LQzHXCCovvnZ7egOPnshp66pp3Z9noFDTHnAb1Iiq4dXngES88WrrMt0F0t6Ohrf04uj79cTE_SuBhDagpZLtMJB6lhFMPAL3ivZpkDQEDdyZJznSnF8YOIYAY6DaWMkJ7lPlMMOhRRWVAdxQuyE9rgXuE08YkpmC9cbjkHeHVeuhIYUnBpuVd5QrI1MrWJSeW4YEZTQ8WCYNYIZo1g1hHMhHwcm8yHmI5_7XyEcI87YsJ2_wPwro68q-_jXULer8lSw9PHYRYVXNvBqdBmBLKQVwl5OZBnPFVR4SqOOWwRW7TaupbtLWH2vU_9xvFXwbKEfBoJeP-tvv4ft_qGPMJjooknL9-SneVN5_ZBii31u_5f9xsCzDUj priority: 102 providerName: Directory of Open Access Journals – databaseName: Unpaywall dbid: UNPAY link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwFLagQ2Iv3C_lJiPxBEqXOI7TPG4V04S0qZpWqTxFji8jInK6tQHKH-Jvck7iRitMIMRjG58m7jm2vxN_5zMhb6xF8qBhQZgpGXDDeFBYVBGNU8bA4Ua3sovHJ-Joxj_Mk_mVWhikVVos3ceDoEvXKQV7ihiOcMiosj27qFBpm7ERJjQZi_YW2t4kOyIBPD4gO7OT6f5HzLSE4AEXbN5tZ15vu7Ugtbr914HN3zmTtxu3kOuvsqquLEiHd4nadKXjoXweNatipL7_ovL4f329R-54vEr3O4P75IZxD8itgxow5foh-dHr1NJznDRpqT37qHU4BURML07PgunklDpEx5Uv-6S1bS2o-eaZuI5Kr49CS0cnjWogAilyjb40S4qlbpf02FR1qevzNRiiqgQynyTd8MnwFzSdLk2jaxhcckl7Fv0jMjt8fzY5CvzRD4GKs2QVjDkAGyUZyovBKh6GRhWQS42zhPMilJLj65eUKZiipFRpZllkQ8lg-kqFBowTPyYDVzvzFIvSxypmNjaR5jyVsDYlJoF4jHmmuZXRkIQb_-fK66Lj8RxVDvkRuiBHF-Togty7YEje9iaLThTkT40PMKj6hqjn3X4B7s69u3MGD5YVhUihm1xkvFAxpMKGM8l1wRI7JK83IZnDv4-bOtKZuoFbIakJQCgXQ_KkC9H-VrHAMyMjuJJuBe_Ws2xfceWnVmMcd3tTFg7Juz7M_97VZ__U-jnZxY_IDYqSF2SwumzMS0B4q-KVH8I_AVPXUkY priority: 102 providerName: Unpaywall |
| Title | Reference genes identification for qRT-PCR normalization of gene expression analysis in Cucumis sativus under Meloidogyne incognita infection and Pseudomonas treatment |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/36589116 https://www.proquest.com/docview/2760172746 https://pubmed.ncbi.nlm.nih.gov/PMC9799720 https://www.frontiersin.org/articles/10.3389/fpls.2022.1061921/pdf https://doaj.org/article/27ae9bb67cb54694bc3828e42a4db25f |
| UnpaywallVersion | publishedVersion |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 1664-462X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: KQ8 dateStart: 20100101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1664-462X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: DOA dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVFQY databaseName: GFMER Free Medical Journals customDbUrl: eissn: 1664-462X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: GX1 dateStart: 20100101 isFulltext: true titleUrlDefault: http://www.gfmer.ch/Medical_journals/Free_medical.php providerName: Geneva Foundation for Medical Education and Research – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1664-462X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: M~E dateStart: 20100101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVAQN databaseName: PubMed Central customDbUrl: eissn: 1664-462X dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: RPM dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.ncbi.nlm.nih.gov/pmc/ providerName: National Library of Medicine – providerCode: PRVFZP databaseName: Scholars Portal Journals: Open Access customDbUrl: eissn: 1664-462X dateEnd: 20250131 omitProxy: true ssIdentifier: ssj0000500997 issn: 1664-462X databaseCode: M48 dateStart: 20100601 isFulltext: true titleUrlDefault: http://journals.scholarsportal.info providerName: Scholars Portal |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3fb9MwED5NG4K9TPwmAyoj8QTKSBwnaR4Q2irGhNSpmlapPEWOY5dKUdK1Dax_Ef8md4kbraLASx9SO3Fy59x38efvAN4aQ-RBzV0vUdIVmgs3M6QiGsSco8F13sguDi-ji7H4Ogkne7Apb2Uf4HJnakf1pMaL4uT2Zv0JJ_xHyjgx3n4w84KEtzk_ofwmoW3lBxioEqrkMLRov5X6JjzUlFuJIuGKiE_adc7dZzmE-0FERfeoFvqdoNVo--8CpH_yKh_U5Vyuf8qiuBO0zh_CkUWb7LR1j0ewp8vHcO-sQkS4fgK_OpVZNqVXHpvlljvUmIshnmU3V9fuaHDFSsK2hd20ySrT9GD61vJoSyatugmblWxQqxr9hxFT6Ee9ZLRRbcGGuqhmeTVdY0fShCDekmQbNhidIWejpa7zCqeGXLKOA_8UxuefrwcXri3c4KogCVduXyAsUZKTOBjGYM_TKsNMqJ-EQmSelII-nsRc4QtGShUnhvvGkxxfPnGUI0IJnsF-WZX6BW0p76uAm0D7uRCxxMgS6hC9KRBJLoz0HfA2lkmVVTWn4hpFitkN2TUlu6Zk19Ta1YF3XZd5K-nxr8ZnZO6uIalxNweqxTS1kzvlOLAky6IYb1NEichUgImsFlyKPOOhceDNxllSfPq0JCNLXdV4KaIkIYQUkQPPW-fpLrVxPgfiLbfaGsv2P-Xse6MQTmu1MfcceN854P9v9fivI3gJh9SQWDx--Ar2V4tav0Ystsp6zTcM_P0y8XvNbOvBwfhydPrtN17nNTY |
| linkProvider | Scholars Portal |
| linkToUnpaywall | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwFLagQ2Iv3C_lJiPxBEqXOI7TPG4V04S0qZpWqTxFji8jInK6tQHKH-Jvck7iRitMIMRjG58m7jm2vxN_5zMhb6xF8qBhQZgpGXDDeFBYVBGNU8bA4Ua3sovHJ-Joxj_Mk_mVWhikVVos3ceDoEvXKQV7ihiOcMiosj27qFBpm7ERJjQZi_YW2t4kOyIBPD4gO7OT6f5HzLSE4AEXbN5tZ15vu7Ugtbr914HN3zmTtxu3kOuvsqquLEiHd4nadKXjoXweNatipL7_ovL4f329R-54vEr3O4P75IZxD8itgxow5foh-dHr1NJznDRpqT37qHU4BURML07PgunklDpEx5Uv-6S1bS2o-eaZuI5Kr49CS0cnjWogAilyjb40S4qlbpf02FR1qevzNRiiqgQynyTd8MnwFzSdLk2jaxhcckl7Fv0jMjt8fzY5CvzRD4GKs2QVjDkAGyUZyovBKh6GRhWQS42zhPMilJLj65eUKZiipFRpZllkQ8lg-kqFBowTPyYDVzvzFIvSxypmNjaR5jyVsDYlJoF4jHmmuZXRkIQb_-fK66Lj8RxVDvkRuiBHF-Togty7YEje9iaLThTkT40PMKj6hqjn3X4B7s69u3MGD5YVhUihm1xkvFAxpMKGM8l1wRI7JK83IZnDv4-bOtKZuoFbIakJQCgXQ_KkC9H-VrHAMyMjuJJuBe_Ws2xfceWnVmMcd3tTFg7Juz7M_97VZ__U-jnZxY_IDYqSF2SwumzMS0B4q-KVH8I_AVPXUkY |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Reference+genes+identification+for+qRT-PCR+normalization+of+gene+expression+analysis+in+Cucumis+sativus+under+Meloidogyne+incognita+infection+and+Pseudomonas+treatment&rft.jtitle=Frontiers+in+plant+science&rft.au=Ji%2C+Tingting&rft.au=Ma%2C+Si&rft.au=Liang%2C+Meiting&rft.au=Wang%2C+Xueyun&rft.date=2022-12-15&rft.issn=1664-462X&rft.eissn=1664-462X&rft.volume=13&rft.spage=1061921&rft_id=info:doi/10.3389%2Ffpls.2022.1061921&rft_id=info%3Apmid%2F36589116&rft.externalDocID=36589116 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-462X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-462X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-462X&client=summon |