Characterization of a secreted aminopeptidase of M28 family from B. fragilis and its possible role in protein metabolism in the gut

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 5; p. 130598
• Main Authors: Kulkarni, Bhushan S., Makde, Ravindra D., Jamdar, Sahayog N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2024
• Subjects: active sites; Amino Acids; Aminopeptidase; Aminopeptidases; Bacteroides fragilis; colon; domain; Endopeptidases; Escherichia coli; family; Humans; M28 family; Peptides; protein metabolism; proteinases; small intestine; soy protein; Soybean protein; species; β-Casein; β-Casein; VpAP; OPA; SgAP; Bacteroides fragilis; PaAP; M28 family; X-βNA; BfAP; EcAP; PDB; AAP; M28; LpAP; Aminopeptidase; Soybean protein; BtAP; OMVs; BsAP
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2024.130598

Products of microbial protein metabolism in the gut can influence the health of the host in many ways. Members of the Bacteriodales, major commensals of the human colon have been associated with long-term intake of high-protein diets. Undigested proteins or peptides that reach the colon can be hydrolyzed by extra-cellular proteases found in some Bacteroides species into amino acids and peptides which can be further catabolized. In this communication, we have characterized one such secreted aminopeptidase (BfAP) from Bacteroides fragilis belonging to the M28 family which is capable of degrading peptides released from soybean protein after predigestion in the small intestine. The BfAP enzyme was cloned, expressed in E. coli, and purified to homogeneity. It is a metallopeptidase requiring Co2+ ion for optimum activity at 55 °C and pH 8 and preferentially cleaves neutral aliphatic (Met/Leu) and positively charged (Arg/Lys) amino acids from the N-terminus of peptides. It showed high specificity for long peptides as well as proteins like β-casein. Structural analysis of BfAP and its orthologues using AlphaFold2 reveal a shared highly conserved M28 domain, but vary with respect to their N-terminal region with some of them possessing an additional cap domain which may be important for regulation of substrate binding. Although BfAP lacks the typical cap domain, it shows small extensions that can form a loop adjacent to the proposed active site and may affect substrate binding. We suggest that this secreted enzyme may play an important role in protein metabolism in the colon where Bacteroides species are abundant. •Extracellular aminopeptidase from B. fragilis was cloned and expressed in E. coli.•It efficiently cleaves Leu, Met, Arg, and Lys residues from the N-terminus of peptides.•Processes wide range of peptides with length 3–12 amino acids long•Enzyme hydrolyzes soy-peptides released after gastrointestinal digestion.