Trypsin-Immobilized Metal-Organic Framework as a Biocatalyst In Proteomics Analysis
Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)‐activated metal–organic frameworks (MOFs). After separation by nano‐LC‐MS2 (liquid chromatography–mass spectrometry), detection, and database searching the protein digestion efficiency of tryp...
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| Published in | ChemPlusChem (Weinheim, Germany) Vol. 77; no. 11; pp. 982 - 986 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Weinheim
WILEY-VCH Verlag
01.11.2012
WILEY‐VCH Verlag |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2192-6506 2192-6506 |
| DOI | 10.1002/cplu.201200186 |
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| Abstract | Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)‐activated metal–organic frameworks (MOFs). After separation by nano‐LC‐MS2 (liquid chromatography–mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin‐MOF was comparable to traditional in‐solution digestion (see figure). Furthermore, the trypsin‐MOF was reusable. |
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| AbstractList | Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)‐activated metal–organic frameworks (MOFs). After separation by nano‐LC‐MS2 (liquid chromatography–mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin‐MOF was comparable to traditional in‐solution digestion (see figure). Furthermore, the trypsin‐MOF was reusable. |
| Author | Chang, I-Hsin Lo, Sheng-Han Singco, Brenda Shih, Yung-Han Cheng, Yi-Jie Wu, Cheng-You Yang, Ni-Shin Huang, Hsi-Ya Lin, Chia-Her |
| Author_xml | – sequence: 1 givenname: Yung-Han surname: Shih fullname: Shih, Yung-Han organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 2 givenname: Sheng-Han surname: Lo fullname: Lo, Sheng-Han organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 3 givenname: Ni-Shin surname: Yang fullname: Yang, Ni-Shin organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 4 givenname: Brenda surname: Singco fullname: Singco, Brenda organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 5 givenname: Yi-Jie surname: Cheng fullname: Cheng, Yi-Jie organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 6 givenname: Cheng-You surname: Wu fullname: Wu, Cheng-You organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 7 givenname: I-Hsin surname: Chang fullname: Chang, I-Hsin organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 8 givenname: Hsi-Ya surname: Huang fullname: Huang, Hsi-Ya email: hyhuang@cycu.edu.tw organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 – sequence: 9 givenname: Chia-Her surname: Lin fullname: Lin, Chia-Her email: chiaher@cycu.edu.tw organization: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399 |
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| Snippet | Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)‐activated metal–organic frameworks (MOFs). After... |
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| SubjectTerms | enzyme immobilization mass spectrometry metal-organic framework nanobiocatalysis proteomics |
| Title | Trypsin-Immobilized Metal-Organic Framework as a Biocatalyst In Proteomics Analysis |
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