Pinoresinol diglucoside is screened as a putative α-glucosidase inhibiting compound in Actinidia arguta leaves

Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. arguta were screened and identified through α-glucosidase-g...

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Published inApplied biological chemistry Vol. 57; no. 4; pp. 473 - 479
Main Authors Kwon, Dodan, Kim, Gyoung Deuck, Kang, Wonseok, Park, Jeong-Eun, Kim, Song Hee, Choe, Eunok, Kim, Jung-In, Auh, Joong-Hyuck
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Applied Biological Chemistry 01.08.2014
Springer Nature B.V
한국응용생명화학회
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Online AccessGet full text
ISSN1738-2203
2468-0834
2234-344X
2234-344X
2468-0842
DOI10.1007/s13765-014-4167-0

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Abstract Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. arguta were screened and identified through α-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting α-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as α-glucosidase inhibitory agents, pinoresinol diglucoside was proposed to be a putative key compound for α-glucosidase inhibition in A. arguta . This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural α-glucosidase inhibitor.
AbstractList Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. arguta were screened and identified through α-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting α-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as α-glucosidase inhibitory agents, pinoresinol diglucoside was proposed to be a putative key compound for α-glucosidase inhibition in A. arguta . This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural α-glucosidase inhibitor.
Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. arguta were screened and identified through α-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting α-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as α-glucosidase inhibitory agents, pinoresinol diglucoside was proposed to be a putative key compound for α-glucosidase inhibition in A. arguta. This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural α-glucosidase inhibitor.
Actinidia arguta leaves are consumed as a popularfood material in Korea and have been reported to exert beneficialeffects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. argutawere screened and identified through α-glucosidase-guidedfractionation and metabolomic analysis. The 50% ethanol extractsof A. arguta showed strong inhibitory effect (32.6%), which wascomparable to acarbose as a positive control (30.0%). Throughmultiple steps of fractionation, pinoresinol diglucoside and fertaricacid were identified as the major potent compounds in A. argutainhibiting α-glucosidase activity by liquid chromatography massspectrometry analysis and metabolomic comparison. Particularly,because pinoresinol and its glycosides have been demonstrated asα-glucosidase inhibitory agents, pinoresinol diglucoside wasproposed to be a putative key compound for α-glucosidaseinhibition in A. arguta. This is the first study demonstrating theanti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural α-glucosidase inhibitor. KCI Citation Count: 8
Author Kim, Song Hee
Kim, Jung-In
Kwon, Dodan
Kang, Wonseok
Park, Jeong-Eun
Auh, Joong-Hyuck
Kim, Gyoung Deuck
Choe, Eunok
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  givenname: Dodan
  surname: Kwon
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  surname: Kim
  fullname: Kim, Gyoung Deuck
  organization: Department of Food Science and Technology, Chung-Ang University
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  surname: Kang
  fullname: Kang, Wonseok
  organization: Department of Food Science and Technology, Chung-Ang University
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  surname: Park
  fullname: Park, Jeong-Eun
  organization: Department of Food Science and Technology, Chung-Ang University
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  givenname: Song Hee
  surname: Kim
  fullname: Kim, Song Hee
  organization: Department of Food Science and Technology, Chung-Ang University
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  surname: Choe
  fullname: Choe, Eunok
  organization: Department of Food and Nutrition, Inha University
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  surname: Kim
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  organization: Department of Smart Foods and Drugs, School of Food and Life Science, Inje University
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  givenname: Joong-Hyuck
  surname: Auh
  fullname: Auh, Joong-Hyuck
  email: jhauh@cau.ac.kr
  organization: Department of Food Science and Technology, Chung-Ang University
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pinoresinol diglucoside
α-glucosidase
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Snippet Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent...
Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent...
Actinidia arguta leaves are consumed as a popularfood material in Korea and have been reported to exert beneficialeffects on humans due to its constituent...
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SubjectTerms Acarbose
Actinidia arguta
Applied Microbiology
Biological Techniques
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Diabetes mellitus
Ethanol
Fractionation
Glucosidase
Glycosides
Inhibitors
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolomics
Polyphenols
α-Glucosidase
농학
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Title Pinoresinol diglucoside is screened as a putative α-glucosidase inhibiting compound in Actinidia arguta leaves
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