The IGH locus relocalizes to a “recombination compartment” in the perinucleolar region of differentiating B-lymphocytes
The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events...
Saved in:
| Published in | Oncotarget Vol. 8; no. 25; pp. 40079 - 40089 |
|---|---|
| Main Authors | , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Impact journals
20.06.2017
Impact Journals LLC |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1949-2553 1949-2553 |
| DOI | 10.18632/oncotarget.16941 |
Cover
| Abstract | The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events rely on the activity of activation-induced cytidine deaminase (AID) which requires DNA double strand breaks be created, a potential danger to the cell. Applying 3D-fluorescence in situ hybridization coupled with immunofluorescence staining to a previously described experimental system recapitulating normal B-cell differentiation ex vivo, we have kinetically analyzed the radial positioning of the two IGH gene loci as well as their proximity with the nucleolus, heterochromatin and γH2AX foci. Our observations are consistent with the proposal that these IGH gene rearrangements take place in a specific perinucleolar "recombination compartment" where AID could be sequestered thus limiting the extent of its potentially deleterious off-target effects. |
|---|---|
| AbstractList | The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events rely on the activity of activation-induced cytidine deaminase (AID) which requires DNA double strand breaks be created, a potential danger to the cell. Applying 3D-fluorescence in situ hybridization coupled with immunofluorescence staining to a previously described experimental system recapitulating normal B-cell differentiation ex vivo, we have kinetically analyzed the radial positioning of the two IGH gene loci as well as their proximity with the nucleolus, heterochromatin and γH2AX foci. Our observations are consistent with the proposal that these IGH gene rearrangements take place in a specific perinucleolar "recombination compartment" where AID could be sequestered thus limiting the extent of its potentially deleterious off-target effects. The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events rely on the activity of activation-induced cytidine deaminase (AID) which requires DNA double strand breaks be created, a potential danger to the cell. Applying 3D-fluorescence in situ hybridization coupled with immunofluorescence staining to a previously described experimental system recapitulating normal B-cell differentiation ex vivo, we have kinetically analyzed the radial positioning of the two IGH gene loci as well as their proximity with the nucleolus, heterochromatin and γH2AX foci. Our observations are consistent with the proposal that these IGH gene rearrangements take place in a specific perinucleolar "recombination compartment" where AID could be sequestered thus limiting the extent of its potentially deleterious off-target effects.The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events rely on the activity of activation-induced cytidine deaminase (AID) which requires DNA double strand breaks be created, a potential danger to the cell. Applying 3D-fluorescence in situ hybridization coupled with immunofluorescence staining to a previously described experimental system recapitulating normal B-cell differentiation ex vivo, we have kinetically analyzed the radial positioning of the two IGH gene loci as well as their proximity with the nucleolus, heterochromatin and γH2AX foci. Our observations are consistent with the proposal that these IGH gene rearrangements take place in a specific perinucleolar "recombination compartment" where AID could be sequestered thus limiting the extent of its potentially deleterious off-target effects. |
| Author | Lipinski, Marc Sklyar, Ilya Fest, Thierry Ivashkin, Evgeny Gavrilov, Alexey Pichugin, Andrey Razin, Sergey V. Caron, Gersende Vassetzky, Yegor S. Iarovaia, Olga V. Barinova, Natalja Barinov, Aleksandr Aoufouchi, Said |
| AuthorAffiliation | 1 UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France 2 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia 6 UMR8200 CNRS, Université Paris-Sud, Institut de Cancérologie Gustave Roussy, Villejuif, France 8 Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia 3 LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France 4 Department of Experimental Neurocytology, Research Center of Neurology, Branch of Brain Research, Moscow, Russia 7 Moscow State University, Moscow, Russia 5 INSERM U1236, CHU de Rennes, Université Rennes 1, Rennes, France |
| AuthorAffiliation_xml | – name: 7 Moscow State University, Moscow, Russia – name: 1 UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France – name: 4 Department of Experimental Neurocytology, Research Center of Neurology, Branch of Brain Research, Moscow, Russia – name: 2 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia – name: 6 UMR8200 CNRS, Université Paris-Sud, Institut de Cancérologie Gustave Roussy, Villejuif, France – name: 3 LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – name: 8 Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia – name: 5 INSERM U1236, CHU de Rennes, Université Rennes 1, Rennes, France |
| Author_xml | – sequence: 1 givenname: Andrey surname: Pichugin fullname: Pichugin, Andrey organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia – sequence: 2 givenname: Olga V. surname: Iarovaia fullname: Iarovaia, Olga V. organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 3 givenname: Alexey surname: Gavrilov fullname: Gavrilov, Alexey organization: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 4 givenname: Ilya surname: Sklyar fullname: Sklyar, Ilya organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 5 givenname: Natalja surname: Barinova fullname: Barinova, Natalja organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 6 givenname: Aleksandr surname: Barinov fullname: Barinov, Aleksandr organization: LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 7 givenname: Evgeny surname: Ivashkin fullname: Ivashkin, Evgeny organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France, Department of Experimental Neurocytology, Research Center of Neurology, Branch of Brain Research, Moscow, Russia – sequence: 8 givenname: Gersende surname: Caron fullname: Caron, Gersende organization: INSERM U1236, CHU de Rennes, Université Rennes 1, Rennes, France – sequence: 9 givenname: Said surname: Aoufouchi fullname: Aoufouchi, Said organization: UMR8200 CNRS, Université Paris-Sud, Institut de Cancérologie Gustave Roussy, Villejuif, France – sequence: 10 givenname: Sergey V. surname: Razin fullname: Razin, Sergey V. organization: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France, Moscow State University, Moscow, Russia – sequence: 11 givenname: Thierry surname: Fest fullname: Fest, Thierry organization: INSERM U1236, CHU de Rennes, Université Rennes 1, Rennes, France – sequence: 12 givenname: Marc surname: Lipinski fullname: Lipinski, Marc organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France – sequence: 13 givenname: Yegor S. surname: Vassetzky fullname: Vassetzky, Yegor S. organization: UMR8126, CNRS, Université Paris Sud Paris Saclay, Institut Gustave Roussy, Villejuif, France, LIA 1066, Laboratoire Franco-Russe de Recherche en Oncologie, Villejuif, France, Moscow State University, Moscow, Russia |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28445143$$D View this record in MEDLINE/PubMed https://univ-rennes.hal.science/hal-01560456$$DView record in HAL |
| BookMark | eNp1ks1u1DAUhS1UREvpA7BBXsIiJf5L4g1SW5VOpZHYlLXlOPaMkWMH26k0sOmD0Jfrk-DOtFAq4c29ss_5fKR7X4M9H7wG4C2qj1HXEPwxeBWyjCudj1HDKXoBDhCnvMKMkb0n_T44SulbXQ6jbYf5K7CPO0oZouQA_Lxaa3h5sYAuqDnBqEuVzv7QCeYAJby7-RW1CmNvvcw2eFj6ScY8ap_vbm6h9TAXwqSj9bNyOjgZC2V1Lw0GDtYYHYvWFrdfwdPKbcZpHdQm6_QGvDTSJX30UA_B18_nV2eLavnl4vLsZFkpwutctWwwTA9YqrbpCFdGkr5vetUOXDJDdMvJ0DFlOk4MbsmAWcMbiltN674QenIIPu2409yPelAlTpROTNGOMm5EkFb8--LtWqzCtWAMY0xJAXzYAdbPbIuTpbi_qxFrasqaa1S07x8-i-H7rFMWo01KOye9DnMSqOO4xaxr6iJ99zTXH_LjdIqg3QlUDClFbYSyeTuGEtM6gWqxXQXxdxXEdhWKEz1zPsL_7_kNQCK_bg |
| CitedBy_id | crossref_primary_10_1134_S0006297918040119 crossref_primary_10_3390_cancers13235949 crossref_primary_10_1002_jmv_28633 crossref_primary_10_1038_s41598_020_75160_z crossref_primary_10_1002_jcp_28219 crossref_primary_10_1016_j_tcb_2019_04_003 crossref_primary_10_1016_j_molimm_2022_03_003 |
| Cites_doi | 10.1016/1074-7613(95)90061-6 10.1073/pnas.1315581110 10.4049/jimmunol.1200301 10.1016/j.molcel.2012.06.036 10.1016/j.cell.2012.04.035 10.1016/S0092-8674(02)01004-8 10.1126/science.1068768 10.1186/1471-213X-12-30 10.1038/nrm2277 10.1016/j.coi.2011.02.002 10.1038/nri.2016.2 10.1038/ni.1735 10.4049/jimmunol.1401849 10.3109/08916934.2012.749244 10.1146/annurev.biochem.72.121801.161724 10.1038/nrm2184 10.1146/annurev.genet.39.073003.110544 10.1128/MCB.9.12.5594 10.1038/ni0901-848 10.1038/sj.onc.1204640 10.1002/jcb.25516 10.1016/B978-0-12-394280-7.00005-1 10.1101/sqb.2010.75.005 10.1371/journal.pone.0026844 10.1101/cshperspect.a000646 10.1016/j.cell.2013.01.007 10.1093/nar/gkg719 10.1182/blood-2013-06-510511 10.7124/bc.00091C 10.4049/jimmunol.173.3.1842 10.1016/j.tig.2011.01.002 10.1242/jcs.107.8.2191 10.1016/j.jmb.2012.11.026 10.1084/jem.20070950 10.1016/j.celrep.2015.08.034 10.1073/pnas.0409491102 10.1093/nar/gkt491 10.1016/j.jmb.2009.10.031 10.1016/bs.ircmb.2014.11.004 10.1016/j.gde.2011.11.004 |
| ContentType | Journal Article |
| Copyright | Distributed under a Creative Commons Attribution 4.0 International License Copyright: © 2017 Pichugin et al. 2017 |
| Copyright_xml | – notice: Distributed under a Creative Commons Attribution 4.0 International License – notice: Copyright: © 2017 Pichugin et al. 2017 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 1XC VOOES 5PM |
| DOI | 10.18632/oncotarget.16941 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| EISSN | 1949-2553 |
| EndPage | 40089 |
| ExternalDocumentID | PMC5522243 oai_HAL_hal_01560456v1 28445143 10_18632_oncotarget_16941 |
| Genre | Journal Article |
| GroupedDBID | --- 53G AAYXX ADBBV ADRAZ AENEX ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL CITATION DIK FRJ GX1 HYE KQ8 M48 OK1 PGMZT RPM CGR CUY CVF ECM EIF NPM 7X8 1XC VOOES 5PM |
| ID | FETCH-LOGICAL-c390t-75df5ed2ac76839cfa3bb6bc7d9a5f3e793d85cf893f273d25696427e40bc39b3 |
| IEDL.DBID | M48 |
| ISSN | 1949-2553 |
| IngestDate | Tue Sep 30 15:43:59 EDT 2025 Fri Sep 12 12:45:59 EDT 2025 Thu Jul 10 18:59:40 EDT 2025 Thu Apr 03 07:01:32 EDT 2025 Tue Jul 01 03:28:02 EDT 2025 Thu Apr 24 23:03:48 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 25 |
| Keywords | differentiation recombination chromatin Chromosome Section immunoglobulin genes CHROMATIN Differentiation |
| Language | English |
| License | Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c390t-75df5ed2ac76839cfa3bb6bc7d9a5f3e793d85cf893f273d25696427e40bc39b3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-2530-5081 0000-0003-3101-7043 0000-0002-6437-4189 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.18632/oncotarget.16941 |
| PMID | 28445143 |
| PQID | 1892725860 |
| PQPubID | 23479 |
| PageCount | 11 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_5522243 hal_primary_oai_HAL_hal_01560456v1 proquest_miscellaneous_1892725860 pubmed_primary_28445143 crossref_citationtrail_10_18632_oncotarget_16941 crossref_primary_10_18632_oncotarget_16941 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2017-06-20 |
| PublicationDateYYYYMMDD | 2017-06-20 |
| PublicationDate_xml | – month: 06 year: 2017 text: 2017-06-20 day: 20 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Oncotarget |
| PublicationTitleAlternate | Oncotarget |
| PublicationYear | 2017 |
| Publisher | Impact journals Impact Journals LLC |
| Publisher_xml | – name: Impact journals – name: Impact Journals LLC |
| References | Ribrag (24) 2014; 123 Vassetzky (25) 2016; 117 Schwer (29) 2013; 152 Singh (19) 2002; 296 Singh (32) 2012; 149 Cook (14) 1994; 107 Pandolfi (9) 2007; 8 Vassetzky (33) 2016; 32 Hwang (35) 2015; 3 Cook (13) 2010; 395 Reynaud (3) 2011; 23 Lamond (8) 2011; 3 Beaujean (41) 2012; 12 Schatz (18) 2009; 10 Fest (21) 2012; 189 Stavnezer (31) 1989; 9 Kavli (23) 2013; 425 Schrader (2) 2014; 193 Längst (36) 2011; 27 Thon (34) 2013; 110 Casali (26) 2013; 46 Reynaud (27) 2008; 205 McBride (5) 2012; 113 Gall (10) 2010; 75 Di Noia (7) 2016; 16 Spector (16) 2003; 72 Cogné (39) 2004; 173 Baltimore (22) 1995; 3 Lamond (37) 2007; 8 de Laat (38) 2013; 41 Maizels (1) 2005; 39 Corces (12) 2002; 111 Roth (6) 2015; 12 Li (11) 2012; 22 Razin (17) 2015; 315 Fisher (20) 2001; 2 Honjo (28) 2005; 102 Dalla-Favera (30) 2001; 20 Skok (4) 2012; 47 Baldacci (15) 2003; 31 Vassetzky (40) 2011; 6 11607811 - Oncogene. 2001 Sep 10;20(40):5580-94 24191010 - Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4465-73 2511438 - Mol Cell Biol. 1989 Dec;9(12):5594-601 23181381 - Autoimmunity. 2013 Mar;46(2):83-101 20926517 - Cold Spring Harb Perspect Biol. 2011 Feb 01;3(2):null 19852969 - J Mol Biol. 2010 Jan 8;395(1):1-10 25411432 - J Immunol. 2014 Dec 1;193(11):5370-8 22132080 - PLoS One. 2011;6(11):e26844 21439803 - Curr Opin Immunol. 2011 Jun;23(3):337-44 16285851 - Annu Rev Genet. 2005;39:23-46 21047905 - Cold Spring Harb Symp Quant Biol. 2010;75:313-20 17928811 - Nat Rev Mol Cell Biol. 2007 Dec;8(12 ):1006-16 26898111 - Nat Rev Immunol. 2016 Mar;16(3):164-76 17519961 - Nat Rev Mol Cell Biol. 2007 Jul;8(7):574-85 23748562 - Nucleic Acids Res. 2013 Aug;41(14):6905-16 8777717 - Immunity. 1995 Dec;3(6):715-26 12930957 - Nucleic Acids Res. 2003 Sep 1;31(17):5064-73 22634617 - J Immunol. 2012 Jul 1;189(1):161-73 26873538 - J Cell Biochem. 2016 Jul;117(7):1506-10 12408858 - Cell. 2002 Oct 18;111(2):151-4 22429855 - Adv Cancer Res. 2012;113:167-90 24452204 - Blood. 2014 Mar 27;123(13):2044-53 11526401 - Nat Immunol. 2001 Sep;2(9):848-54 22178420 - Curr Opin Genet Dev. 2012 Apr;22(2):101-9 26365182 - Cell Rep. 2015 Sep 22;12(11):1842-52 18474627 - J Exp Med. 2008 Jun 9;205(6):1357-68 15684068 - Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2022-7 19448632 - Nat Immunol. 2009 Jun;10(6):655-64 23183374 - J Mol Biol. 2013 Jan 23;425(2):424-43 23095683 - BMC Dev Biol. 2012 Oct 24;12:30 14527325 - Annu Rev Biochem. 2003;72:573-608 7983177 - J Cell Sci. 1994 Aug;107 ( Pt 8):2191-202 25708464 - Int Rev Cell Mol Biol. 2015;315:183-244 15265916 - J Immunol. 2004 Aug 1;173(3):1842-8 26104555 - Microbiol Spectr. 2015 Feb;3(1):MDNA3-0037-2014 11935030 - Science. 2002 Apr 5;296(5565):158-62 21295884 - Trends Genet. 2011 Apr;27(4):149-56 22726435 - Cell. 2012 Jun 22;149(7):1474-87 23374339 - Cell. 2013 Jan 31;152(3):417-29 22864115 - Mol Cell. 2012 Sep 28;47(6):873-85 |
| References_xml | – volume: 3 start-page: 715 year: 1995 ident: 22 article-title: Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2 publication-title: Immunity doi: 10.1016/1074-7613(95)90061-6 – volume: 110 start-page: E4465 year: 2013 ident: 34 article-title: Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.1315581110 – volume: 189 start-page: 161 year: 2012 ident: 21 article-title: IL-2 requirement for human plasma cell generation: coupling differentiation and proliferation by enhancing MAPK-ERK signaling publication-title: J Immunol doi: 10.4049/jimmunol.1200301 – volume: 47 start-page: 873 year: 2012 ident: 4 article-title: Close proximity to Igh is a contributing factor to AID-mediated translocations publication-title: Mol Cell doi: 10.1016/j.molcel.2012.06.036 – volume: 149 start-page: 1474 year: 2012 ident: 32 article-title: DNA sequence-dependent compartmentalization and silencing of chromatin at the nuclear lamina publication-title: Cell doi: 10.1016/j.cell.2012.04.035 – volume: 111 start-page: 151 year: 2002 ident: 12 article-title: Setting the boundaries of chromatin domains and nuclear organization publication-title: Cell doi: 10.1016/S0092-8674(02)01004-8 – volume: 296 start-page: 158 year: 2002 ident: 19 article-title: Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development publication-title: Science doi: 10.1126/science.1068768 – volume: 12 start-page: 30 year: 2012 ident: 41 article-title: 3D-FISH analysis of embryonic nuclei in mouse highlights several abrupt changes of nuclear organization during preimplantation development publication-title: BMC Dev Biol doi: 10.1186/1471-213X-12-30 – volume: 8 start-page: 1006 year: 2007 ident: 9 article-title: Structure, dynamics and functions of promyelocytic leukaemia nuclear bodies publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm2277 – volume: 23 start-page: 337 year: 2011 ident: 3 article-title: AID and partners: for better and (not) for worse publication-title: Curr Opin Immunol doi: 10.1016/j.coi.2011.02.002 – volume: 16 start-page: 164 year: 2016 ident: 7 article-title: Mutations, kataegis and translocations in B cells: understanding AID promiscuous activity publication-title: Nat Rev Immunol doi: 10.1038/nri.2016.2 – volume: 10 start-page: 655 year: 2009 ident: 18 article-title: RAG-1 and ATM coordinate monoallelic recombination and nuclear positioning of immunoglobulin loci publication-title: Nat Immunol doi: 10.1038/ni.1735 – volume: 193 start-page: 5370 year: 2014 ident: 2 article-title: IgH Chain Class Switch Recombination: Mechanism and Regulation publication-title: J Immunol doi: 10.4049/jimmunol.1401849 – volume: 46 start-page: 83 year: 2013 ident: 26 article-title: Regulation of Aicda expression and AID activity publication-title: Autoimmunity doi: 10.3109/08916934.2012.749244 – volume: 72 start-page: 573 year: 2003 ident: 16 article-title: The dynamics of chromosome organization and gene regulation publication-title: Annu Rev Biochem doi: 10.1146/annurev.biochem.72.121801.161724 – volume: 8 start-page: 574 year: 2007 ident: 37 article-title: The multifunctional nucleolus publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm2184 – volume: 39 start-page: 23 year: 2005 ident: 1 article-title: Immunoglobulin gene diversification publication-title: Annu Rev Genet doi: 10.1146/annurev.genet.39.073003.110544 – volume: 9 start-page: 5594 year: 1989 ident: 31 article-title: A B-cell-specific nuclear protein that binds to DNA sites 5’ to immunoglobulin S alpha tandem repeats is regulated during differentiation publication-title: Mol Cell Biol doi: 10.1128/MCB.9.12.5594 – volume: 2 start-page: 848 year: 2001 ident: 20 article-title: Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes publication-title: Nat Immunol doi: 10.1038/ni0901-848 – volume: 3 year: 2015 ident: 35 article-title: Related Mechanisms of Antibody Somatic Hypermutation and Class Switch Recombination publication-title: Microbiol Spectr – volume: 20 start-page: 5580 year: 2001 ident: 30 article-title: Mechanisms of chromosomal translocations in B cell lymphomas publication-title: Oncogene doi: 10.1038/sj.onc.1204640 – volume: 117 start-page: 1506 year: 2016 ident: 25 article-title: Distinct Patterns of Colocalization of the CCND1 and CMYC Genes With Their Potential Translocation Partner IGH at Successive Stages of B-Cell Differentiation publication-title: J Cell Biochem doi: 10.1002/jcb.25516 – volume: 113 start-page: 167 year: 2012 ident: 5 article-title: Activation-induced cytidine deaminase in antibody diversification and chromosome translocation publication-title: Adv Cancer Res doi: 10.1016/B978-0-12-394280-7.00005-1 – volume: 75 start-page: 313 year: 2010 ident: 10 article-title: Cajal bodies and histone locus bodies in Drosophila and Xenopus publication-title: Cold Spring Harb Symp Quant Biol doi: 10.1101/sqb.2010.75.005 – volume: 6 start-page: e26844 year: 2011 ident: 40 article-title: Ring-like distribution of constitutive heterochromatin in bovine senescent cells publication-title: PLoS One doi: 10.1371/journal.pone.0026844 – volume: 3 year: 2011 ident: 8 article-title: Nuclear speckles publication-title: Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a000646 – volume: 152 start-page: 417 year: 2013 ident: 29 article-title: Mechanisms of programmed DNA lesions and genomic instability in the immune system publication-title: Cell doi: 10.1016/j.cell.2013.01.007 – volume: 31 start-page: 5064 year: 2003 ident: 15 article-title: Nuclear factories for signalling and repairing DNA double strand breaks in living fission yeast publication-title: Nucleic Acids Res doi: 10.1093/nar/gkg719 – volume: 123 start-page: 2044 year: 2014 ident: 24 article-title: Perinucleolar relocalization and nucleolin as crucial events in the transcriptional activation of key genes in mantle cell lymphoma publication-title: Blood doi: 10.1182/blood-2013-06-510511 – volume: 32 start-page: 179 year: 2016 ident: 33 article-title: Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human B-cell maturation publication-title: Biopolym Cell doi: 10.7124/bc.00091C – volume: 173 start-page: 1842 year: 2004 ident: 39 article-title: Transcription-dependent somatic hypermutation occurs at similar levels on functional and nonfunctional rearranged IgH alleles publication-title: J Immunol doi: 10.4049/jimmunol.173.3.1842 – volume: 27 start-page: 149 year: 2011 ident: 36 article-title: Genome organization in and around the nucleolus publication-title: Trends Genet doi: 10.1016/j.tig.2011.01.002 – volume: 107 start-page: 2191 year: 1994 ident: 14 article-title: Replication factories and nuclear bodies: the ultrastructural characterization of replication sites during the cell cycle publication-title: J Cell Sci doi: 10.1242/jcs.107.8.2191 – volume: 425 start-page: 424 year: 2013 ident: 23 article-title: A combined nuclear and nucleolar localization motif in activation-induced cytidine deaminase (AID) controls immunoglobulin class switching publication-title: J Mol Biol doi: 10.1016/j.jmb.2012.11.026 – volume: 205 start-page: 1357 year: 2008 ident: 27 article-title: Proteasomal degradation restricts the nuclear lifespan of AID publication-title: J Exp Med doi: 10.1084/jem.20070950 – volume: 12 start-page: 1842 year: 2015 ident: 6 article-title: Off-Target V(D)J Recombination Drives Lymphomagenesis and Is Escalated by Loss of the Rag2 C Terminus publication-title: Cell Rep doi: 10.1016/j.celrep.2015.08.034 – volume: 102 start-page: 2022 year: 2005 ident: 28 article-title: DNA cleavage in immunoglobulin somatic hypermutation depends on de novo protein synthesis but not on uracil DNA glycosylase publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0409491102 – volume: 41 start-page: 6905 year: 2013 ident: 38 article-title: Allelic exclusion of the immunoglobulin heavy chain locus is independent of its nuclear localization in mature B cells publication-title: Nucleic Acids Res doi: 10.1093/nar/gkt491 – volume: 395 start-page: 1 year: 2010 ident: 13 article-title: A model for all genomes: the role of transcription factories publication-title: J Mol Biol doi: 10.1016/j.jmb.2009.10.031 – volume: 315 start-page: 183 year: 2015 ident: 17 article-title: Nuclear compartments, genome folding, and enhancer-promoter communication publication-title: Int Rev Cell Mol Biol doi: 10.1016/bs.ircmb.2014.11.004 – volume: 22 start-page: 101 year: 2012 ident: 11 article-title: A view of nuclear Polycomb bodies publication-title: Curr Opin Genet Dev doi: 10.1016/j.gde.2011.11.004 – reference: 18474627 - J Exp Med. 2008 Jun 9;205(6):1357-68 – reference: 24191010 - Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4465-73 – reference: 17928811 - Nat Rev Mol Cell Biol. 2007 Dec;8(12 ):1006-16 – reference: 15265916 - J Immunol. 2004 Aug 1;173(3):1842-8 – reference: 26365182 - Cell Rep. 2015 Sep 22;12(11):1842-52 – reference: 26873538 - J Cell Biochem. 2016 Jul;117(7):1506-10 – reference: 22864115 - Mol Cell. 2012 Sep 28;47(6):873-85 – reference: 20926517 - Cold Spring Harb Perspect Biol. 2011 Feb 01;3(2):null – reference: 12930957 - Nucleic Acids Res. 2003 Sep 1;31(17):5064-73 – reference: 17519961 - Nat Rev Mol Cell Biol. 2007 Jul;8(7):574-85 – reference: 2511438 - Mol Cell Biol. 1989 Dec;9(12):5594-601 – reference: 25411432 - J Immunol. 2014 Dec 1;193(11):5370-8 – reference: 22726435 - Cell. 2012 Jun 22;149(7):1474-87 – reference: 21439803 - Curr Opin Immunol. 2011 Jun;23(3):337-44 – reference: 12408858 - Cell. 2002 Oct 18;111(2):151-4 – reference: 11526401 - Nat Immunol. 2001 Sep;2(9):848-54 – reference: 23374339 - Cell. 2013 Jan 31;152(3):417-29 – reference: 11607811 - Oncogene. 2001 Sep 10;20(40):5580-94 – reference: 23095683 - BMC Dev Biol. 2012 Oct 24;12:30 – reference: 23183374 - J Mol Biol. 2013 Jan 23;425(2):424-43 – reference: 19852969 - J Mol Biol. 2010 Jan 8;395(1):1-10 – reference: 26898111 - Nat Rev Immunol. 2016 Mar;16(3):164-76 – reference: 11935030 - Science. 2002 Apr 5;296(5565):158-62 – reference: 26104555 - Microbiol Spectr. 2015 Feb;3(1):MDNA3-0037-2014 – reference: 23181381 - Autoimmunity. 2013 Mar;46(2):83-101 – reference: 23748562 - Nucleic Acids Res. 2013 Aug;41(14):6905-16 – reference: 14527325 - Annu Rev Biochem. 2003;72:573-608 – reference: 16285851 - Annu Rev Genet. 2005;39:23-46 – reference: 22634617 - J Immunol. 2012 Jul 1;189(1):161-73 – reference: 22178420 - Curr Opin Genet Dev. 2012 Apr;22(2):101-9 – reference: 22132080 - PLoS One. 2011;6(11):e26844 – reference: 25708464 - Int Rev Cell Mol Biol. 2015;315:183-244 – reference: 15684068 - Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2022-7 – reference: 22429855 - Adv Cancer Res. 2012;113:167-90 – reference: 21295884 - Trends Genet. 2011 Apr;27(4):149-56 – reference: 8777717 - Immunity. 1995 Dec;3(6):715-26 – reference: 7983177 - J Cell Sci. 1994 Aug;107 ( Pt 8):2191-202 – reference: 21047905 - Cold Spring Harb Symp Quant Biol. 2010;75:313-20 – reference: 24452204 - Blood. 2014 Mar 27;123(13):2044-53 – reference: 19448632 - Nat Immunol. 2009 Jun;10(6):655-64 |
| SSID | ssj0000547829 |
| Score | 2.1975842 |
| Snippet | The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and... |
| SourceID | pubmedcentral hal proquest pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 40079 |
| SubjectTerms | B-Lymphocytes - immunology B-Lymphocytes - metabolism Cancer Cell Differentiation - immunology Cell Line, Tumor Cell Nucleolus - immunology Cell Nucleolus - metabolism Cells, Cultured Cytidine Deaminase - immunology Cytidine Deaminase - metabolism Germinal Center - cytology Germinal Center - immunology Germinal Center - metabolism Humans Immunoglobulin Class Switching - genetics Immunoglobulin Class Switching - immunology Immunoglobulin Heavy Chains - genetics Immunoglobulin Heavy Chains - immunology Immunoglobulin Heavy Chains - metabolism Immunology In Situ Hybridization, Fluorescence - methods Life Sciences Lymphocyte Activation - immunology Microscopy, Confocal Research Paper: Chromosome Somatic Hypermutation, Immunoglobulin - genetics Somatic Hypermutation, Immunoglobulin - immunology |
| Title | The IGH locus relocalizes to a “recombination compartment” in the perinucleolar region of differentiating B-lymphocytes |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/28445143 https://www.proquest.com/docview/1892725860 https://univ-rennes.hal.science/hal-01560456 https://pubmed.ncbi.nlm.nih.gov/PMC5522243 |
| Volume | 8 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 1949-2553 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000547829 issn: 1949-2553 databaseCode: KQ8 dateStart: 20100101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries – providerCode: PRVBFR databaseName: Free Medical Journals customDbUrl: eissn: 1949-2553 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000547829 issn: 1949-2553 databaseCode: DIK dateStart: 20100101 isFulltext: true titleUrlDefault: http://www.freemedicaljournals.com providerName: Flying Publisher – providerCode: PRVFQY databaseName: GFMER Free Medical Journals customDbUrl: eissn: 1949-2553 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000547829 issn: 1949-2553 databaseCode: GX1 dateStart: 20100101 isFulltext: true titleUrlDefault: http://www.gfmer.ch/Medical_journals/Free_medical.php providerName: Geneva Foundation for Medical Education and Research – providerCode: PRVAQN databaseName: PubMed Central customDbUrl: eissn: 1949-2553 dateEnd: 99991231 omitProxy: true ssIdentifier: ssj0000547829 issn: 1949-2553 databaseCode: RPM dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.ncbi.nlm.nih.gov/pmc/ providerName: National Library of Medicine – providerCode: PRVFZP databaseName: Scholars Portal Journals: Open Access customDbUrl: eissn: 1949-2553 dateEnd: 20250131 omitProxy: true ssIdentifier: ssj0000547829 issn: 1949-2553 databaseCode: M48 dateStart: 20100501 isFulltext: true titleUrlDefault: http://journals.scholarsportal.info providerName: Scholars Portal |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9NAEB415cIFFfEKfWhBnJBcnH34cahQQbQBUU5Eys1a74NGCjYkTkXg0h8Cf66_hJm1EwhUSFzt9Vreb8bzjXb2G4AnUhmM2y6LrMTcRJoSXYonPuJ5RgJamVOcTiOfvUuGI_lmrMZbsGpv1S3g_NrUjvpJjWbTwy-fl8_R4Y_I4bNE8Gc16RiEwunDAR3M7MENDEycjPysY_ut1LfEeJiHfWaZR0imRbfPee0sG5Gqd051kn-T0D9rKX8LTic7cKtjley4NYPbsOWqO_ANTYC9Ph0yjFeLOZu5ELcmX92cNTXT7OryO6XDHzE3DvCwth49lJ1fXf5gk4ohO2QkhVyR6jGtFKNGDji09mzVWqUhcKsP7EU0XaJp1GaJ7PUujE5evX85jLpeC5ERedxEqbJeOcu1wfxD5MZrUZZJaVKba-WFQze2mTIe6Y1HxmORKeWYuqROxiXOUIp7sF3VlXsAzIvSaoc0TQotFaZzlns9cCKx3nGlkz7Eq3UtTCdETv0wpgUlJARF8QuKIkDRh6frRz61Khz_GvwYwVqPI_3s4fHbgq6Fc-NIGS9w0KMVlgU6FO2S6MrVizlOmvOUqyyJ-3C_xXY9F8ZySQyzD-kG6hsv27xTTc6DaLdCosulePg_X7ILNzmxiDjBn9kebDezhdtHDtSUB9A7HQ8Ogn3_BHOODho |
| linkProvider | Scholars Portal |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+IGH+locus+relocalizes+to+a+%E2%80%9Crecombination+compartment%E2%80%9D+in+the+perinucleolar+region+of+differentiating+B-lymphocytes&rft.jtitle=Oncotarget&rft.au=Pichugin%2C+Andrey&rft.au=Iarovaia%2C+Olga+V.&rft.au=Gavrilov%2C+Alexey&rft.au=Sklyar%2C+Ilya&rft.date=2017-06-20&rft.issn=1949-2553&rft.eissn=1949-2553&rft.volume=8&rft.issue=25&rft.spage=40079&rft.epage=40089&rft_id=info:doi/10.18632%2Foncotarget.16941&rft.externalDBID=n%2Fa&rft.externalDocID=10_18632_oncotarget_16941 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1949-2553&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1949-2553&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1949-2553&client=summon |