The use of microdialysis to characterize the endocrine production of human subcutaneous adipose tissue in vivo

• Published in: Regulatory peptides Vol. 155; no. 1; pp. 156 - 162
• Main Authors: Dostálová, Ivana, Kaválková, Petra, Haluzíková, Denisa, Housová, Jitka, Matoulek, Martin, Haluzík, Martin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 05.06.2009; Elsevier
• Subjects: Adipokines; Adipokines - metabolism; Adiponectin - metabolism; Biological and medical sciences; Diurnal rhythm; Female; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - metabolism; Humans; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Leptin - metabolism; Microdialysis; Microdialysis - methods; Plasminogen Activator Inhibitor 1 - metabolism; Proinflammatory cytokines; Resistin - metabolism; Subcutaneous Fat - metabolism; Trauma; Vertebrates: endocrinology; Young Adult; Diurnal rhythm; Microdialysis; Proinflammatory cytokines; Glucose; Adipokines; Trauma; Human; In vivo; Adipose tissue; Subcutaneous; Cytokine; Adipokine
• ISSN: 0167-0115; 1873-1686; 1873-1686
• DOI: 10.1016/j.regpep.2009.03.008

Adipose tissue-derived factors represent important players in the metabolic regulations acting both on systemic and local level. However, their local concentrations in human adipose tissue are poorly described. We measured 24-hour profile and post-glucose load concentrations of selected adipokines in the subcutaneous adipose tissue of 17 healthy women by in vivo microdialysis. During 24-hour period, subjects consumed two standardized meals (at 13.00 h and at 19.00 h). During 24-hour period, fat interleukin-6, interleukin-8/CXCL8, resistin, and hepatocyte growth factor (HGF) exhibited increase/decrease/plateau pattern and peaked at about 14.30 h. Fat leptin exhibited increase/plateau/decrease/increase pattern and reached plateau between 22.00 and 5.30 h. Fat adiponectin exhibited decrease/plateau pattern and reached plateau between 1.00 and 7.00 h. Fat plasminogen activator inhibitor-1 (PAI-1) exhibited decrease/increase pattern with the lowest value at 20.30 h. Oral glucose consumption significantly increased fat adiponectin and resistin levels and decreased fat leptin and PAI-1 levels, respectively. The levels of studied adipokines in subcutaneous fat exhibited significant variations during the 24-hour period after microdialysis catheter insertion that were not reflected in the circulation. Concentrations of adiponectin, resistin, leptin and PAI-1 were regulated by oral glucose ingestion from 1 to 3 h after oral glucose load in healthy women.