Triptolide-induced in vitro and in vivo cytotoxicity in human breast cancer stem cells and primary breast cancer cells
We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and...
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| Published in | Oncology reports Vol. 31; no. 5; pp. 2181 - 2186 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Greece
D.A. Spandidos
01.05.2014
Spandidos Publications UK Ltd |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1021-335X 1791-2431 1791-2431 |
| DOI | 10.3892/or.2014.3115 |
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| Abstract | We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and BCSCs from invasive ductal carcinoma samples were cultured and treated with 0.1, 0.5 or 1.0 μM triptolide. Cell death and apoptosis were measured after 24, 48 and 72 h of treatment. Mammospheres were found to be highly tumorigenic when implanted subcutaneously in nude BALB/c mice. Triptolide was cytotoxic against both human primary BCCs and BCSCs in vitro (P<0.05), but the cytotoxicity was stronger against the BCCs. In response to 1 μM triptolide for 72 h, the apoptotic rates were approximately 60% for BCCs and 30% for BCSCs. The BCSCs exhibited a high formation rate of tumors when implanted subcutaneously in nude BALB/c mice. Triptolide treatment in vivo significantly inhibited tumor growth compared with mock treatment. In conclusion, the cytotoxicity of triptolide against BCCs and BCSCs in vitro and in vivo suggests that this natural diterpenoid triepoxide compound may have clinical applications for the suppression of breast tumor growth. |
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| AbstractList | We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and BCSCs from invasive ductal carcinoma samples were cultured and treated with 0.1, 0.5 or 1.0 μM triptolide. Cell death and apoptosis were measured after 24, 48 and 72 h of treatment. Mammospheres were found to be highly tumorigenic when implanted subcutaneously in nude BALB/c mice. Triptolide was cytotoxic against both human primary BCCs and BCSCs in vitro (P<0.05), but the cytotoxicity was stronger against the BCCs. In response to 1 μM triptolide for 72 h, the apoptotic rates were approximately 60% for BCCs and 30% for BCSCs. The BCSCs exhibited a high formation rate of tumors when implanted subcutaneously in nude BALB/c mice. Triptolide treatment in vivo significantly inhibited tumor growth compared with mock treatment. In conclusion, the cytotoxicity of triptolide against BCCs and BCSCs in vitro and in vivo suggests that this natural diterpenoid triepoxide compound may have clinical applications for the suppression of breast tumor growth. We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and BCSCs from invasive ductal carcinoma samples were cultured and treated with 0.1, 0.5 or 1.0 µM triptolide. Cell death and apoptosis were measured after 24, 48 and 72 h of treatment. Mammospheres were found to be highly tumorigenic when implanted subcutaneously in nude BALB/c mice. Triptolide was cytotoxic against both human primary BCCs and BCSCs in vitro (P<0.05), but the cytotoxicity was stronger against the BCCs. In response to 1 µM triptolide for 72 h, the apoptotic rates were approximately 60% for BCCs and 30% for BCSCs. The BCSCs exhibited a high formation rate of tumors when implanted subcutaneously in nude BALB/c mice. Triptolide treatment in vivo significantly inhibited tumor growth compared with mock treatment. In conclusion, the cytotoxicity of triptolide against BCCs and BCSCs in vitro and in vivo suggests that this natural diterpenoid triepoxide compound may have clinical applications for the suppression of breast tumor growth. We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and BCSCs from invasive ductal carcinoma samples were cultured and treated with 0.1, 0.5 or 1.0 µM triptolide. Cell death and apoptosis were measured after 24, 48 and 72 h of treatment. Mammospheres were found to be highly tumorigenic when implanted subcutaneously in nude BALB/c mice. Triptolide was cytotoxic against both human primary BCCs and BCSCs in vitro (P<0.05), but the cytotoxicity was stronger against the BCCs. In response to 1 µM triptolide for 72 h, the apoptotic rates were approximately 60% for BCCs and 30% for BCSCs. The BCSCs exhibited a high formation rate of tumors when implanted subcutaneously in nude BALB/c mice. Triptolide treatment in vivo significantly inhibited tumor growth compared with mock treatment. In conclusion, the cytotoxicity of triptolide against BCCs and BCSCs in vitro and in vivo suggests that this natural diterpenoid triepoxide compound may have clinical applications for the suppression of breast tumor growth.We investigated the potential efficacy of the Chinese herbal extract triptolide for the treatment of human breast cancer by measuring the triptolide-induced cytotoxicity in cultures of human primary breast cancer cells (BCCs) and breast cancer stem cells (BCSCs) in vitro and in vivo. Human BCCs and BCSCs from invasive ductal carcinoma samples were cultured and treated with 0.1, 0.5 or 1.0 µM triptolide. Cell death and apoptosis were measured after 24, 48 and 72 h of treatment. Mammospheres were found to be highly tumorigenic when implanted subcutaneously in nude BALB/c mice. Triptolide was cytotoxic against both human primary BCCs and BCSCs in vitro (P<0.05), but the cytotoxicity was stronger against the BCCs. In response to 1 µM triptolide for 72 h, the apoptotic rates were approximately 60% for BCCs and 30% for BCSCs. The BCSCs exhibited a high formation rate of tumors when implanted subcutaneously in nude BALB/c mice. Triptolide treatment in vivo significantly inhibited tumor growth compared with mock treatment. In conclusion, the cytotoxicity of triptolide against BCCs and BCSCs in vitro and in vivo suggests that this natural diterpenoid triepoxide compound may have clinical applications for the suppression of breast tumor growth. |
| Author | LI, JUNJIE LIU, RUILEI YANG, YE LI, XI SHEN, XIAOYAN HUANG, YONG LIU, RUIMING |
| Author_xml | – sequence: 1 givenname: JUNJIE surname: LI fullname: LI, JUNJIE organization: Department of Breast Surgery, Sichuan Cancer Hospital, Chengdu 610041, P.R. China – sequence: 2 givenname: RUILEI surname: LIU fullname: LIU, RUILEI organization: Department of Breast Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, P.R. China – sequence: 3 givenname: YE surname: YANG fullname: YANG, YE organization: Department of Pulmonary Tumor, Sichuan Cancer Hospital, Chengdu 610041, P.R. China – sequence: 4 givenname: YONG surname: HUANG fullname: HUANG, YONG organization: Department of Breast Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, P.R. China – sequence: 5 givenname: XI surname: LI fullname: LI, XI organization: Department of Breast Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, P.R. China – sequence: 6 givenname: RUIMING surname: LIU fullname: LIU, RUIMING organization: Laboratory of Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P.R. China – sequence: 7 givenname: XIAOYAN surname: SHEN fullname: SHEN, XIAOYAN organization: Laboratory of Pharmacology and Toxicology, College of Pharmacy, Sun Yat-sen University, Guangzhou 510006, P.R. China |
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| SubjectTerms | Animals Antineoplastic Agents, Alkylating - pharmacology Apoptosis Apoptosis - drug effects Breast cancer breast cancer cells breast cancer stem cells Breast Neoplasms - drug therapy Cancer therapies Carcinoma, Ductal, Breast - drug therapy Cell culture Cytotoxicity Diterpenes - pharmacology Drugs, Chinese Herbal - pharmacology Epidermal growth factor Epoxy Compounds - pharmacology Female Genotype & phenotype Humans Humidity Immunoglobulins Leukemia Metastasis Mice Mice, Inbred BALB C Mice, Nude Neoplastic Stem Cells - drug effects Phenanthrenes - pharmacology Spheroids, Cellular - transplantation Stem cells Studies triptolide Tumor Cells, Cultured Tumors Xenograft Model Antitumor Assays |
| Title | Triptolide-induced in vitro and in vivo cytotoxicity in human breast cancer stem cells and primary breast cancer cells |
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