Development of novel digital PCR assays for the rapid quantification of Gram-negative bacteria biomarkers using RUCS algorithm - Bogožalec Košir, Alexandra; Alič, Špela; Tomič, Viktorija; Lužnik, Dane; Dreo, Tanja; Milavec, Mojca (Methods (San Diego, Calif.), 2024)
Keywords: Acinetobacter baumannii; Acinetobacter baumannii - genetics; Acinetobacter baumannii - isolation & purification; Algorithms; Biomarkers; Biomarkers - analysis; cross reaction; detection limit; diagnostic techniques; Digital PCR (dPCR); disease control; DNA; DNA, Bacterial - analysis; DNA, Bacterial - genetics; genome; Gram-negative bacteria; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - diagnosis; Gram-Negative Bacterial Infections - microbiology; hospitals; Humans; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; matrix-assisted laser desorption-ionization mass spectrometry; Pathogen detection; Polymerase Chain Reaction - methods; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification; Reproducibility of Results; Respiratory infections; RUCS algorithm; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; uncertainty; Biomarkers; Pathogen detection; Digital PCR (dPCR); Gram-negative bacteria; RUCS algorithm; Respiratory infections