Effect of Yishen Huayu Fang on kidney tissue E-cadherin expression in unilateral ureter ligation in rats
OBJECTIVE：To observe E-calcium sticky protein（E-cadherin） expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang（formula of tonifying the kidney and dissolving accumulated blood stasis） on the expression.METHODS：A total of 150 clean grade male r...
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| Published in | Journal of Traditional Chinese Medicine Vol. 32; no. 2; pp. 273 - 277 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
China
01.06.2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0255-2922 0254-6272 |
| DOI | 10.1016/S0254-6272(13)60024-2 |
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| Abstract | OBJECTIVE：To observe E-calcium sticky protein（E-cadherin） expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang（formula of tonifying the kidney and dissolving accumulated blood stasis） on the expression.METHODS：A total of 150 clean grade male rats were randomly divided into a control group,model group,low-dose Yishen Huayu Fang group（low-dose group）,high-dose Yishen Huayu Fang group（high-dose group）,and Lotensin group.A renal fibrosis model was established with unilateral ureteral obstruction（UUO）.Pathological changes of rat renal tissue were observed with light microscopy on days 3,7,14,21,and 28 after UUO.Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.RESULTS：Three days after modeling,kidney edema appeared followed by gradual inflammatory cell infiltration,and part of the small tubules disappeared while the renal cortex thinned.Meanwhile,the E-cadherin expression level dropped,which was negatively correlated with the obstruction time.After intervention,E-cadherin expression was increased in all treatment groups（P〈0.01 or P〈0.05）,while there were no significant differences between the high-dose and Lotensin groups.CONCLUSION：Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition. |
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| AbstractList | OBJECTIVE：To observe E-calcium sticky protein（E-cadherin） expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang（formula of tonifying the kidney and dissolving accumulated blood stasis） on the expression.METHODS：A total of 150 clean grade male rats were randomly divided into a control group,model group,low-dose Yishen Huayu Fang group（low-dose group）,high-dose Yishen Huayu Fang group（high-dose group）,and Lotensin group.A renal fibrosis model was established with unilateral ureteral obstruction（UUO）.Pathological changes of rat renal tissue were observed with light microscopy on days 3,7,14,21,and 28 after UUO.Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.RESULTS：Three days after modeling,kidney edema appeared followed by gradual inflammatory cell infiltration,and part of the small tubules disappeared while the renal cortex thinned.Meanwhile,the E-cadherin expression level dropped,which was negatively correlated with the obstruction time.After intervention,E-cadherin expression was increased in all treatment groups（P〈0.01 or P〈0.05）,while there were no significant differences between the high-dose and Lotensin groups.CONCLUSION：Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition. To observe E-calcium sticky protein (E-cadherin) expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang (formula of tonifying the kidney and dissolving accumulated blood stasis) on the expression.OBJECTIVETo observe E-calcium sticky protein (E-cadherin) expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang (formula of tonifying the kidney and dissolving accumulated blood stasis) on the expression.A total of 150 clean grade male rats were randomly divided into a control group, model group, low-dose Yishen Huayu Fang group (low-dose group), high-dose Yishen Huayu Fang group (high-dose group), and Lotensin group. A renal fibrosis model was established with unilateral ureteral obstruction (UUO). Pathological changes of rat renal tissue were observed with light microscopy on days 3, 7, 14, 21, and 28 after UUO. Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.METHODSA total of 150 clean grade male rats were randomly divided into a control group, model group, low-dose Yishen Huayu Fang group (low-dose group), high-dose Yishen Huayu Fang group (high-dose group), and Lotensin group. A renal fibrosis model was established with unilateral ureteral obstruction (UUO). Pathological changes of rat renal tissue were observed with light microscopy on days 3, 7, 14, 21, and 28 after UUO. Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.Three days after modeling, kidney edema appeared followed by gradual inflammatory cell infiltration, and part of the small tubules disappeared while the renal cortex thinned. Meanwhile, the E-cadherin expression level dropped, which was negatively correlated with the obstruction time. After intervention, E-cadherin expression was increased in all treatment groups (P < 0.01 or P < 0.05), while there were no significant differences between the high-dose and Lotensin groups.RESULTSThree days after modeling, kidney edema appeared followed by gradual inflammatory cell infiltration, and part of the small tubules disappeared while the renal cortex thinned. Meanwhile, the E-cadherin expression level dropped, which was negatively correlated with the obstruction time. After intervention, E-cadherin expression was increased in all treatment groups (P < 0.01 or P < 0.05), while there were no significant differences between the high-dose and Lotensin groups.Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition.CONCLUSIONYishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition. To observe E-calcium sticky protein (E-cadherin) expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang (formula of tonifying the kidney and dissolving accumulated blood stasis) on the expression. A total of 150 clean grade male rats were randomly divided into a control group, model group, low-dose Yishen Huayu Fang group (low-dose group), high-dose Yishen Huayu Fang group (high-dose group), and Lotensin group. A renal fibrosis model was established with unilateral ureteral obstruction (UUO). Pathological changes of rat renal tissue were observed with light microscopy on days 3, 7, 14, 21, and 28 after UUO. Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry. Three days after modeling, kidney edema appeared followed by gradual inflammatory cell infiltration, and part of the small tubules disappeared while the renal cortex thinned. Meanwhile, the E-cadherin expression level dropped, which was negatively correlated with the obstruction time. After intervention, E-cadherin expression was increased in all treatment groups (P < 0.01 or P < 0.05), while there were no significant differences between the high-dose and Lotensin groups. Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition. |
| Author | 张琳琪 李伟明 孙伟 |
| AuthorAffiliation | The First Clinical Medical College of Nanjing University of Traditional Chinese Medicine, Nanjing, Ji-angsu 210046, China TCM Internal Medicine Discipline, The Henan Institute of Traditional Chinese Medicine, Zhengzhou, Henan 450008, China |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/22876456$$D View this record in MEDLINE/PubMed |
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| Notes | Yishen Huayu Fang Formula of tonifying the kidney and dissolving accumulated blood stasis Renal fibrosis Unilateral ureter ligation E-calcium mucin 11-2167/R OBJECTIVE：To observe E-calcium sticky protein（E-cadherin） expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang（formula of tonifying the kidney and dissolving accumulated blood stasis） on the expression.METHODS：A total of 150 clean grade male rats were randomly divided into a control group,model group,low-dose Yishen Huayu Fang group（low-dose group）,high-dose Yishen Huayu Fang group（high-dose group）,and Lotensin group.A renal fibrosis model was established with unilateral ureteral obstruction（UUO）.Pathological changes of rat renal tissue were observed with light microscopy on days 3,7,14,21,and 28 after UUO.Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.RESULTS：Three days after modeling,kidney edema appeared followed by gradual inflammatory cell infiltration,and part of the small tubules disappeared while the renal cortex thinned.Meanwhile,the E-cadherin expression level dropped,which was negatively correlated with the obstruction time.After intervention,E-cadherin expression was increased in all treatment groups（P〈0.01 or P〈0.05）,while there were no significant differences between the high-dose and Lotensin groups.CONCLUSION：Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
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| SubjectTerms | Animals Cadherins - analysis Dose-Response Relationship, Drug Drugs, Chinese Herbal Fibrosis Immunohistochemistry Kidney - chemistry Kidney - pathology Male Medicine, Chinese Traditional Rats Rats, Sprague-Dawley Ureteral Obstruction - drug therapy Ureteral Obstruction - metabolism 免疫组化法 单侧 大鼠模型 显微镜观察 结扎 肾组织 肾间质纤维化 输尿管 |
| Title | Effect of Yishen Huayu Fang on kidney tissue E-cadherin expression in unilateral ureter ligation in rats |
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