Characterization of FcγRIIIA effector cells used in in vitro ADCC bioassay: Comparison of primary NK cells with engineered NK-92 and Jurkat T cells - Hsieh, Yao-Te; Aggarwal, Poonam; Cirelli, David; Gu, Ling; Surowy, Teresa; Mozier, Ned M. (Journal of immunological methods, 2017)
Keywords: ADCC bioassay; antibodies; Antibody-Dependent Cell Cytotoxicity; Antibody-dependent cell-mediated cytotoxicity; bioassays; Biological Assay - methods; Cell Engineering; Cell Line, Tumor; cytotoxicity; dephosphorylation; dose response; drugs; FcγRIIIA; Genotype; Humans; immunoglobulin G; Jurkat Cells; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; manufacturing; mechanism of action; natural killer cells; Primary Cell Culture; Receptors, IgG - immunology; Receptors, IgG - isolation & purification; T-lymphocytes; ADCC bioassay; FACS; T cells; V-158; EGFR; NFAT; MFI; FITC; FcR; FBS; E/T ratio; EC50; Fc; ADCC; MOA; Antibody-dependent cell-mediated cytotoxicity; NK cells; F-158; Fab; ASN-297; IgG1; IL-2; FcγRIIIA; IRDye; PBMC; AAF-Glo; APC; PE; ITAM; mAbs; GAPDH; pNFAT2; Her2