A SNP-based assay in a non model species: evolutionary lineage assignment of brown trout ancient DNA

A set of 12 single nucleotide polymorphisms selected for both confirming Salmo trutta identity and distinguishing the six European evolutionary lineages were included in a multiplex single-base primer extension assay to confidently assign brown trout ancient DNA to one of the six European haplogroup...

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Bibliographic Details
Published inConservation genetics resources Vol. 4; no. 1; pp. 59 - 62
Main Authors Chat, Joëlle, Manicki, Aurélie
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.03.2012
Springer Nature B.V
Springer
Subjects
Animal Genetics and Genomics
Biodiversity
Biodiversity and Ecology
Biomedical and Life Sciences
Conservation Biology/Ecology
Deoxyribonucleic acid
DNA
Ecology
Environmental Sciences
Evolutionary Biology
Life Sciences
Other
Plant Genetics and Genomics
Salmo trutta
Single-nucleotide polymorphism
Technical Note
Ancient DNA
Mitochondrial DNA
Evolutionary lineage
Homoplasy
SNaPshot
Multiplex primer extension
SALMONIDE
GENETIQUE
mitochondrial dna
salmo trutta
poisson
ancient dna
salmonids
truite commune
homplasy
multiplex primer extension
salmonidae
fish
sea trout
evolutionary lineage
snapshot
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ISSN1877-7252
1877-7260
DOI10.1007/s12686-011-9474-8

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Summary:A set of 12 single nucleotide polymorphisms selected for both confirming Salmo trutta identity and distinguishing the six European evolutionary lineages were included in a multiplex single-base primer extension assay to confidently assign brown trout ancient DNA to one of the six European haplogroups. The assignment rate reaches 75% for brown trout scales collected 40 years ago.
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ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-011-9474-8