An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections

Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and valida...

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Published inJournal of immunological methods Vol. 530; p. 113696
Main Authors Donadel, Nicola, Tesser, Alessandra, Valencic, Erica, De Martino, Eleonora, Boz, Valentina, Pin, Alessia, Zorat, Francesca, Pozzato, Gabriele, Tommasini, Alberto
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.07.2024
Subjects
Antibodies, Neutralizing - blood
Antibodies, Neutralizing - immunology
antiviral agents
Autoantibodies
Autoantibodies - blood
Autoantibodies - immunology
COVID-19
COVID-19 - blood
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 infection
Cytokines
Cytokines - blood
Cytokines - immunology
Female
Functional assay
Humans
Interferon
Interferon Type I - immunology
interferons
Male
Middle Aged
Reproducibility of Results
SARS-CoV-2 - immunology
COVID-19
Interferon
Autoantibodies
Cytokines
Functional assay
Online AccessGet full text
ISSN0022-1759
1872-7905
1872-7905
DOI10.1016/j.jim.2024.113696

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Abstract Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and validated to measure the neutralizing potency of autoantibodies directed to type I or type II IFN. Identifying of anti-cytokine autoantibodies might reflect on early treatments for subsequent infections, such as with antivirals or virus-neutralizing monoclonal antibodies.
AbstractList Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and validated to measure the neutralizing potency of autoantibodies directed to type I or type II IFN. Identifying of anti-cytokine autoantibodies might reflect on early treatments for subsequent infections, such as with antivirals or virus-neutralizing monoclonal antibodies.
Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and validated to measure the neutralizing potency of autoantibodies directed to type I or type II IFN. Identifying of anti-cytokine autoantibodies might reflect on early treatments for subsequent infections, such as with antivirals or virus-neutralizing monoclonal antibodies.Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and validated to measure the neutralizing potency of autoantibodies directed to type I or type II IFN. Identifying of anti-cytokine autoantibodies might reflect on early treatments for subsequent infections, such as with antivirals or virus-neutralizing monoclonal antibodies.
ArticleNumber 113696
Author Boz, Valentina
De Martino, Eleonora
Zorat, Francesca
Donadel, Nicola
Pozzato, Gabriele
Tommasini, Alberto
Pin, Alessia
Tesser, Alessandra
Valencic, Erica
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Keywords COVID-19
Interferon
Autoantibodies
Cytokines
Functional assay
Language English
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Copyright © 2023. Published by Elsevier B.V.
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Snippet Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has...
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SubjectTerms Antibodies, Neutralizing - blood
Antibodies, Neutralizing - immunology
antiviral agents
Autoantibodies
Autoantibodies - blood
Autoantibodies - immunology
COVID-19
COVID-19 - blood
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 infection
Cytokines
Cytokines - blood
Cytokines - immunology
Female
Functional assay
Humans
Interferon
Interferon Type I - immunology
interferons
Male
Middle Aged
Reproducibility of Results
SARS-CoV-2 - immunology
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Title An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections
URI https://dx.doi.org/10.1016/j.jim.2024.113696
https://www.ncbi.nlm.nih.gov/pubmed/38797274
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https://doi.org/10.1016/j.jim.2024.113696
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