Exploring the Expression and Function of T Cell Surface Markers Identified through Cellular Indexing of Transcriptomes and Epitopes by Sequencing

• Published in: Yonsei medical journal Vol. 65; no. 9; pp. 544 - 555
• Main Authors: Hwang, Joon Yeon, Kim, Youngtaek, Kim, Dong Kwon, Lee, Seul, Kang, Seong-san, Baek, Sujeong, Yang, Seung Min, Kim, Mi Hyun, Han, Heekyung, Jeong, Seong Su, Lee, Chai Young, Han, Yu Jin, Sohn, Jie-Ohn, Ye, Sang-Kyu, Pyo, Kyoung-Ho
• Format: Journal Article
• Language: English
• Published: Korea (South) Yonsei University College of Medicine 01.09.2024; 연세대학교의과대학
• Subjects: CD4-Positive T-Lymphocytes - metabolism; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; Epitopes - genetics; Epitopes - immunology; Gene Expression Profiling - methods; Humans; Leukocytes, Mononuclear - metabolism; Original; RNA, Messenger - genetics; RNA, Messenger - metabolism; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Transcriptome; 의학일반; single-cell gene expression analysis; T-lymphocyte subsets; CITE-seq; transcriptome; proteome
• ISSN: 0513-5796; 1976-2437; 1976-2437
• DOI: 10.3349/ymj.2023.0639

By utilizing both protein and mRNA expression patterns, we can identify more detailed and diverse immune cells, providing insights into understanding the complex immune landscape in cancer ecosystems. This study was performed by obtaining publicly available Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) data of peripheral blood mononuclear cells (PBMCs) from the Gene Expression Omnibus database. A total of 94674 total cells were analyzed, of which 32412 were T cells. There were 228 protein features and 16262 mRNA features in the data. The Seurat package was used for quality control and preprocessing, principal component analysis was performed, and Uniform Manifold Approximation and Projection was used to visualize the clusters. Protein and mRNA levels in the CITE-seq were analyzed. We observed that a subset of T cells in the clusters generated at the protein level divided better. By identifying mRNA markers that were highly correlated with the CD4 and CD8 proteins and cross-validating CD26 and CD99 markers using flow cytometry, we found that CD4 and CD8 T cells were better discriminated in PBMCs. Weighted Nearest Neighbor clustering results identified a previously unobserved T cell subset. In this study, we used CITE-seq data to confirm that protein expression patterns could be used to identify cells more precisely. These findings will improve our understanding of the heterogeneity of immune cells in the future and provide valuable insights into the complexity of the immune response in health and disease.