Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish
Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophys...
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| Published in | Scientific reports Vol. 8; no. 1; pp. 15986 - 12 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Nature Publishing Group
30.10.2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 2045-2322 2045-2322 |
| DOI | 10.1038/s41598-018-33577-7 |
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| Abstract | Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient
in vivo
ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine. |
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| AbstractList | Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine. Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine.Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine. Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine. |
| ArticleNumber | 15986 |
| Author | Chen, Yu-Fu Lee, Chi-Chun Liu, Lawrence Yu-Min Chuang, Yung-Jen Chou, Huang-Cheng Liu, Wangta Lin, Min-Hsuan |
| Author_xml | – sequence: 1 givenname: Min-Hsuan surname: Lin fullname: Lin, Min-Hsuan – sequence: 2 givenname: Huang-Cheng surname: Chou fullname: Chou, Huang-Cheng – sequence: 3 givenname: Yu-Fu surname: Chen fullname: Chen, Yu-Fu – sequence: 4 givenname: Wangta surname: Liu fullname: Liu, Wangta – sequence: 5 givenname: Chi-Chun surname: Lee fullname: Lee, Chi-Chun – sequence: 6 givenname: Lawrence Yu-Min surname: Liu fullname: Liu, Lawrence Yu-Min – sequence: 7 givenname: Yung-Jen orcidid: 0000-0002-6022-0107 surname: Chuang fullname: Chuang, Yung-Jen |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30375400$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1161/01.CIR.32.4.549 10.1161/01.RES.58.1.109 10.1089/zeb.2015.1208 10.1007/s10439-009-9668-3 10.1021/jm501981g 10.1089/zeb.2010.0653 10.1152/ajpregu.00418.2002 10.1186/1475-925X-3-28 10.1109/Cic.2002.1166717 10.1371/journal.pone.0101606 10.1002/9780470942390.mo100159 10.1111/j.1471-4159.1982.tb04711.x 10.1038/s41598-017-03050-y 10.1038/srep25073 10.3791/1645 10.1016/j.bios.2015.04.027 10.1016/S0002-8703(96)90309-9 10.1016/j.cbpc.2015.03.007 10.1371/journal.pone.0060552 10.1016/j.vascn.2013.10.002 10.1080/19336950.2015.1121335 10.1152/ajpheart.00960.2005 10.1007/s10544-011-9612-9 10.3390/s18010061 10.1056/nejm199801013380107 10.1038/sj.bjp.0703451 10.1016/j.cca.2011.07.002 10.1111/j.1748-1716.2010.02111.x |
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| SubjectTerms | Danio rerio Economics EKG Electrocardiography Electrophysiology Heart Isoflurane Toxicity Zebrafish |
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| Title | Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish |
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