Medium optimization for the production of the aroma compound 2-phenylethanol using a genetic algorithm

Using a genetic algorithm, 13 medium constituents and the temperature were varied to improve the bioconversion of l-phenylalanine (l-phe) to 2-phenylethanol (2-PE) with Kluyveromyces marxianus CBS 600. Within four generations plus an additional temperature screening, corresponding to 98 parallel exp...

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Bibliographic Details
Published inJournal of molecular catalysis. B, Enzymatic Vol. 29; no. 1-6; pp. 187 - 193
Main Authors Etschmann, M.M.W, Sell, D, Schrader, J
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 21.06.2004
Elsevier Science
Subjects
2-Phenylethanol
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Flavor
Fragrance
Fundamental and applied biological sciences. Psychology
Genetic algorithm
Kluyveromyces
Methods. Procedures. Technologies
Yeast
Yeast
Genetic algorithm
Flavor
2-Phenylethanol
Fragrance
Kluyveromyces
Fungi
Production
Ascomycetes
Aroma
Optimization
Thallophyta
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ISSN1381-1177
1873-3158
DOI10.1016/j.molcatb.2003.10.014

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Summary:Using a genetic algorithm, 13 medium constituents and the temperature were varied to improve the bioconversion of l-phenylalanine (l-phe) to 2-phenylethanol (2-PE) with Kluyveromyces marxianus CBS 600. Within four generations plus an additional temperature screening, corresponding to 98 parallel experiments altogether, a maximum 2-PE concentration of 5.6g/l, equivalent to an increase of 87% compared to the non-optimized medium was obtained. The vitamin content of the medium had to be raised significantly.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2003.10.014