Induction of Adhesion Molecule Expression in Co-culture of Human Bronchial Epithelial Cells and Neutrophils Suppressed by Puerarin via Down-regulating p38 Mitogen-Activated Protein Kinase and Nuclear Factor κB Pathways

• Published in: Chinese journal of integrative medicine Vol. 20; no. 5; pp. 360 - 368
• Main Author: 刘野 邵玲俐 庞伟 兰晓梅 吕建新 丛玉隆 王成彬
• Format: Journal Article
• Language: English
• Published: Heidelberg Chinese Association of Traditional and Western Medicine 01.05.2014
• Subjects: Animals; Base Sequence; Bronchi - cytology; Bronchi - enzymology; Bronchi - metabolism; Cattle; Cell Adhesion Molecules - metabolism; Cell Line; Coculture Techniques; DNA Primers; Down-Regulation - drug effects; Epithelial Cells - enzymology; Epithelial Cells - metabolism; Isoflavones - pharmacology; Medicine; Medicine & Public Health; Neutrophils - enzymology; Neutrophils - metabolism; NF-kappa B - metabolism; Original Article; p38 Mitogen-Activated Protein Kinases - metabolism; p38丝裂原活化蛋白激酶; Phosphorylation; Real-Time Polymerase Chain Reaction; 共培养体系; 分子表达; 嗜中性粒细胞; 支气管上皮细胞; 核因子κB; 葛根素; 黏附; puerarin; p38 mitogen-activated protein kinase; bronchial epithelial cells; adhesion molecules; neutrophils; nuclear factor kappa B
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-013-1515-6

Objective： In this study, we aimed to investigate the expressions of adhesion molecules on human bronchial epithelial cells and neutrophils in co-culture system, assess the effects of puerarin on suppressing these adhesion molecules expressions, and explore the roles of two crucial signal-transduction elements p38 mitogen-activated protein kinase （p38 MAPK） and nuclear factor kappa B （NF- K B） in modulating adhesion molecules expressions. Methods： Neutrophils and BEAS-2B cells （one human bronchial epithelial cell line） were co-cultured, and adhesion molecules expressions on cell surface were detected using flow cytometry. The mRNA levels of adhesion molecules were assessed by real-time quantitative polymerase chain reaction （real-time qPCR）. Phosphorylated p38 MAPK and inhibitor K B were analyzed by Western blot. Results： In co-culture system, adhesion molecules expressions on BEAS-2B cells and neutrephils were enhanced significantly （P〈0.05）. Correspondingly, the mRNA levels of adhesion molecules were also increased greatly. Moreover, the pretreatment of peurarin obviously suppressed adhesion molecules expressions on cell surface. Furthermore, phosphorylated p38 MAPK and inhibitor K B in BEAS-2B cells and neutrophils were elevated in co-culture system, but decreased significantly after upon the treatment of peurarin （P〈0.05）. Conclusions： Co- culture boosted the interactions between human bronchial epithelial cells and neutrophils mimicking airway inflammation, whereas peurarin decreased the expression of adhesion molecules on cell surface by suppressing the activities of p38 MAPK and NF- K B pathways, and exhibiting its anti-inflammation activity.