Re-evaluation of CBMN test reference values of persons continuously occupationally exposed to low doses of ionizing radiation in Serbia

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 886; p. 503583
• Main Authors: Pajic, J., Rakic, B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2023
• Subjects: Cytokinesis; Humans; Micronuclei; Micronucleus Tests; Nuclear buds; Nuclear division index; Nucleoplasmic bridges; Occupational exposure; Radiation, Ionizing; Reference Values; Serbia; Serbia; Nuclear division index; Nuclear buds; Occupational exposure; Nucleoplasmic bridges; Micronuclei
• ISSN: 1383-5718; 1879-3592; 1879-3592
• DOI: 10.1016/j.mrgentox.2023.503583

When established, cytokinesis-block micronucleus (CBMN) test reference values should be periodically evaluated according to the recommendations of reference documents. The biodosimetry cytogenetic laboratory of the Serbian Institute of Occupational Health established the CBMN test reference range for people occupationally exposed to ionizing radiation in 2016. Since then, new occupationally exposed persons have been subjected to micronucleus testing, resulting in the need for re-evaluation of existing CBMN test values. The examined population comprised 608 occupationally exposed subjects – 201 from the previous laboratory database and 407 newly examined. Comparison of groups based on gender, age and cigarette consumption did not show significant differences, although certain CBMN values differed significantly between the old and new groups. Duration of occupational exposure, gender, age and smoking habit influenced micronuclei frequency in all three analyzed groups, while no relation was found between type of work and micronucleus test parameters. Since the mean values of all tested parameters in the new group of examinees are within previously established reference ranges, existing values can be used in further research. •When established, CBMN test reference values should be periodically evaluated.•We calculated CBMN test reference values for occupationally exposed persons in 2016.•In subsequent years we subjected new occupationally exposed persons to CBMN testing.•In this study we compare the old and the new database values.•New CBMN test values are within the already established reference range.