Ocular Surface Expression of SARS-CoV-2 Receptors

The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown. Gene expression of SARS-CoV-2 receptors was investigated in c...

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Published inOcular immunology and inflammation Vol. 28; no. 5; pp. 735 - 738
Main Authors Leonardi, Andrea, Rosani, Umberto, Brun, Paola
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 03.07.2020
Subjects
ACE2
ADAR
Adenosine Deaminase - genetics
Adolescent
Adult
Aged
Angiotensin-Converting Enzyme 2
APOBEC3
Basigin - genetics
Betacoronavirus - physiology
CD147
Child
conjunctiva
Conjunctiva - metabolism
cornea
Cornea - metabolism
Coronavirus Infections - metabolism
COVID-19
Cytidine Deaminase - genetics
Gene Expression Profiling
Gene Expression Regulation - physiology
Humans
Middle Aged
Pandemics
Peptidyl-Dipeptidase A - genetics
Pneumonia, Viral - metabolism
Proteins - genetics
Receptors, Virus - genetics
RNA-Binding Proteins - genetics
SARS-CoV-2
Serine Endopeptidases - genetics
TMPRSS2
Young Adult
COVID-19
ACE2
APOBEC3
SARS-CoV-2
cornea
conjunctiva
TMPRSS2
ADAR
CD147
Online AccessGet full text
ISSN0927-3948
1744-5078
1744-5078
DOI10.1080/09273948.2020.1772314

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Abstract The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown. Gene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing. ACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed. The ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement.
AbstractList The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown.PURPOSEThe spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown.Gene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing.MATERIAL AND METHODSGene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing.ACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed.RESULTSACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed.The ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement.CONCLUSIONSThe ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement.
The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown. Gene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing. ACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed. The ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement.
Author Rosani, Umberto
Brun, Paola
Leonardi, Andrea
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  organization: University of Padova
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cornea
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Snippet The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine...
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SubjectTerms ACE2
ADAR
Adenosine Deaminase - genetics
Adolescent
Adult
Aged
Angiotensin-Converting Enzyme 2
APOBEC3
Basigin - genetics
Betacoronavirus - physiology
CD147
Child
conjunctiva
Conjunctiva - metabolism
cornea
Cornea - metabolism
Coronavirus Infections - metabolism
COVID-19
Cytidine Deaminase - genetics
Gene Expression Profiling
Gene Expression Regulation - physiology
Humans
Middle Aged
Pandemics
Peptidyl-Dipeptidase A - genetics
Pneumonia, Viral - metabolism
Proteins - genetics
Receptors, Virus - genetics
RNA-Binding Proteins - genetics
SARS-CoV-2
Serine Endopeptidases - genetics
TMPRSS2
Young Adult
Title Ocular Surface Expression of SARS-CoV-2 Receptors
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