Safety of ovarian tissue transplantation in patients with borderline ovarian tumors

Abstract STUDY QUESTION Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? SUMMARY ANSWER BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients. WHAT IS KNOWN ALREADY The risk of reintroducin...

Full description

Saved in:
Bibliographic Details
Published inHuman reproduction (Oxford) Vol. 33; no. 2; pp. 212 - 219
Main Authors Masciangelo, Rossella, Bosisio, Chiara, Donnez, Jacques, Amorim, Christiani A, Dolmans, Marie-Madeleine
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.02.2018
Subjects
Online AccessGet full text
ISSN0268-1161
1460-2350
1460-2350
DOI10.1093/humrep/dex352

Cover

Abstract Abstract STUDY QUESTION Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? SUMMARY ANSWER BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients. WHAT IS KNOWN ALREADY The risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring. STUDY DESIGN, SIZE, DURATION Prospective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs. PARTICIPANTS/MATERIALS, SETTING, METHODS Histology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample. MAIN RESULTS AND THE ROLE OF CHANCE Analysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10. LIMITATIONS, REASONS FOR CAUTION Cryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells. WIDER IMPLICATIONS OF THE FINDINGS BOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario. STUDY FUNDING/COMPETING INTEREST(S) This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare.
AbstractList Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients. The risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring. Prospective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs. Histology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample. Analysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10. Cryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells. BOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario. This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare.
Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure?STUDY QUESTIONIs transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure?BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients.SUMMARY ANSWERBOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients.The risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring.WHAT IS KNOWN ALREADYThe risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring.Prospective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs.STUDY DESIGN, SIZE, DURATIONProspective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs.Histology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample.PARTICIPANTS/MATERIALS, SETTING, METHODSHistology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample.Analysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10.MAIN RESULTS AND THE ROLE OF CHANCEAnalysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10.Cryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells.LIMITATIONS, REASONS FOR CAUTIONCryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells.BOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario.WIDER IMPLICATIONS OF THE FINDINGSBOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario.This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare.STUDY FUNDING/COMPETING INTEREST(S)This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare.
Abstract STUDY QUESTION Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? SUMMARY ANSWER BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients. WHAT IS KNOWN ALREADY The risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring. STUDY DESIGN, SIZE, DURATION Prospective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs. PARTICIPANTS/MATERIALS, SETTING, METHODS Histology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample. MAIN RESULTS AND THE ROLE OF CHANCE Analysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10. LIMITATIONS, REASONS FOR CAUTION Cryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells. WIDER IMPLICATIONS OF THE FINDINGS BOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario. STUDY FUNDING/COMPETING INTEREST(S) This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare.
Author Bosisio, Chiara
Donnez, Jacques
Dolmans, Marie-Madeleine
Masciangelo, Rossella
Amorim, Christiani A
Author_xml – sequence: 1
  givenname: Rossella
  surname: Masciangelo
  fullname: Masciangelo, Rossella
  organization: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200 Brussels, Belgium
– sequence: 2
  givenname: Chiara
  surname: Bosisio
  fullname: Bosisio, Chiara
  organization: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200 Brussels, Belgium
– sequence: 3
  givenname: Jacques
  surname: Donnez
  fullname: Donnez, Jacques
  organization: Société de Recherche pour l'Infertilité, Avenue Grandchamp 143, 1150 Brussels, Belgium
– sequence: 4
  givenname: Christiani A
  orcidid: 0000-0003-1794-0368
  surname: Amorim
  fullname: Amorim, Christiani A
  organization: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200 Brussels, Belgium
– sequence: 5
  givenname: Marie-Madeleine
  surname: Dolmans
  fullname: Dolmans, Marie-Madeleine
  email: marie-madeleine.dolmans@uclouvain.be
  organization: Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200 Brussels, Belgium
BackLink https://www.ncbi.nlm.nih.gov/pubmed/29281007$$D View this record in MEDLINE/PubMed
BookMark eNqFkE1LxDAQhoMouq4evUqPXuomzTbZHmXxCxY8rJ5Dmk7YSJvUJFX33xvpLoIgnmYYnneYeU7RoXUWELog-Jrgis42Q-ehnzXwScviAE3InOG8oCU-RBNcsEVOCCMn6DSEV4xTu2DH6KSoigXBmE_Qei01xG3mdObepTfSZtGEMEAWvbShb6WNMhpnM2OzPnVgY8g-TNxktfMN-NZY-IkOnfPhDB1p2QY439Uperm7fV4-5Kun-8flzSpXlJUxr7RqeIkVaQjlGApWzzXBjOq6VJQ3jPI0a3Q516XWnFSaS1oBrTHDinOQdIquxr29d28DhCg6ExS06WZwQxCkSk-WRbKU0MsdOtQdNKL3ppN-K_YiEkBHQHkXggctlBkfTx5MKwgW37rFqFuMulMq_5XaL_6L313shv4f9At0eJQP
CitedBy_id crossref_primary_10_1097_OGX_0000000000001325
crossref_primary_10_1186_s12958_024_01266_y
crossref_primary_10_1016_j_ejogrb_2024_07_055
crossref_primary_10_1016_j_fertnstert_2021_03_008
crossref_primary_10_1016_j_glohj_2021_04_005
crossref_primary_10_1111_aogs_13577
crossref_primary_10_2147_IJWH_S258478
crossref_primary_10_1016_j_rbmo_2024_104450
crossref_primary_10_1016_j_ejca_2019_04_018
crossref_primary_10_1016_j_ygyno_2021_03_030
crossref_primary_10_1007_s13669_021_00312_5
crossref_primary_10_1093_hropen_hoad012
crossref_primary_10_3390_biomedicines10092217
crossref_primary_10_1007_s10815_020_01729_2
crossref_primary_10_1016_j_gine_2023_100933
crossref_primary_10_3390_cimb44050157
crossref_primary_10_1007_s00129_018_4347_9
crossref_primary_10_1177_1179558119867357
crossref_primary_10_1093_humrep_deae291
crossref_primary_10_1093_humrep_deaa353
crossref_primary_10_1093_humrep_deaa175
crossref_primary_10_1093_humrep_dez077
crossref_primary_10_1007_s00404_024_07445_x
crossref_primary_10_4274_tjod_galenos_2022_42272
crossref_primary_10_1002_rmb2_12268
crossref_primary_10_23736_S2724_606X_22_05066_7
crossref_primary_10_1016_j_rbmo_2020_06_009
crossref_primary_10_1016_j_ygyno_2024_03_008
crossref_primary_10_1111_jog_13818
crossref_primary_10_3390_jcm9124101
crossref_primary_10_3390_jcm10184233
crossref_primary_10_1016_j_cryobiol_2022_11_240
crossref_primary_10_33667_2078_5631_2024_8_41_47
crossref_primary_10_1093_hropen_hoaa052
Cites_doi 10.1016/S0015-0282(00)00548-3
10.1038/nrendo.2013.205
10.1186/s13048-016-0226-y
10.1111/his.12182
10.1016/j.fertnstert.2013.03.030
10.1182/blood-2010-01-265751
10.1093/humrep/det340
10.1016/j.fertnstert.2009.11.032
10.1093/oxfordjournals.humrep.a138556
10.1016/j.ejca.2005.07.029
10.1016/S0140-6736(04)17222-X
10.1016/j.fertnstert.2014.10.045
10.5402/2011/140310
10.1016/j.fertnstert.2004.10.009
10.1093/humrep/dew193
10.1111/j.1365-2559.2010.03673.x
10.1007/s10815-016-0843-9
10.1016/j.fertnstert.2013.03.027
10.1136/jcp.55.5.391
10.1016/j.fertnstert.2011.05.003
10.1093/humupd/dms047
10.1016/j.ctrv.2016.06.010
10.1093/humrep/dep196
10.1016/j.fertnstert.2006.07.1503
10.1016/j.fertnstert.2017.03.017
10.1007/s10815-015-0544-9
10.1093/humrep/den459
10.1007/s10815-013-0065-3
10.1093/annonc/mdp267
10.1200/JCO.2001.19.10.2658
10.1016/j.bpobgyn.2011.12.006
10.1016/S0015-0282(03)00160-2
ContentType Journal Article
Copyright The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2017
The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com
Copyright_xml – notice: The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2017
– notice: The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1093/humrep/dex352
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Anatomy & Physiology
Pharmacy, Therapeutics, & Pharmacology
EISSN 1460-2350
EndPage 219
ExternalDocumentID 29281007
10_1093_humrep_dex352
10.1093/humrep/dex352
Genre Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: Fonds National de la Recherche Scientifique de Belgique
  grantid: T.0077.14; 7.4590.16; 5/4/150/5
GroupedDBID ---
-E4
.2P
.I3
.XZ
.ZR
0R~
1TH
29I
2WC
4.4
482
48X
53G
5GY
5RE
5VS
5WA
5WD
70D
AABZA
AACZT
AAIMJ
AAJKP
AAJQQ
AAMDB
AAMVS
AAOGV
AAPNW
AAPQZ
AAPXW
AARHZ
AAUAY
AAUQX
AAVAP
AAVLN
ABDFA
ABEJV
ABEUO
ABGNP
ABIXL
ABJNI
ABKDP
ABMNT
ABNHQ
ABNKS
ABPQP
ABPTD
ABQLI
ABQNK
ABVGC
ABWST
ABXVV
ABXZS
ABZBJ
ACCCW
ACGFS
ACPRK
ACUFI
ACUTJ
ACUTO
ACYHN
ADBBV
ADEYI
ADEZT
ADGKP
ADGZP
ADHKW
ADHZD
ADIPN
ADNBA
ADOCK
ADQBN
ADRTK
ADVEK
ADYVW
ADZXQ
AEGPL
AEJOX
AEKSI
AELWJ
AEMDU
AEMQT
AENEX
AENZO
AEPUE
AETBJ
AEWNT
AFFZL
AFGWE
AFIYH
AFOFC
AFXAL
AGINJ
AGKEF
AGQXC
AGSYK
AGUTN
AHMBA
AHMMS
AHXPO
AIJHB
AJEEA
AJNCP
AKWXX
ALMA_UNASSIGNED_HOLDINGS
ALUQC
ALXQX
APIBT
APWMN
ARIXL
ATGXG
AXUDD
AYOIW
BAWUL
BAYMD
BCRHZ
BEYMZ
BHONS
BQDIO
BSWAC
BTRTY
BVRKM
C45
CDBKE
CS3
CZ4
DAKXR
DIK
DILTD
DU5
D~K
E3Z
EBS
EE~
EJD
EMOBN
ENERS
F5P
F9B
FECEO
FHSFR
FLUFQ
FOEOM
FOTVD
FQBLK
GAUVT
GJXCC
GX1
H13
H5~
HAR
HW0
HZ~
IOX
J21
JXSIZ
KAQDR
KBUDW
KOP
KQ8
KSI
KSN
L7B
M-Z
M49
MHKGH
ML0
N9A
NGC
NLBLG
NOMLY
NOYVH
NU-
O9-
OAUYM
OAWHX
OBOKY
OCZFY
ODMLO
OJQWA
OJZSN
OK1
OPAEJ
OVD
OWPYF
P2P
PAFKI
PEELM
PQQKQ
Q1.
Q5Y
R44
RD5
ROL
ROX
ROZ
RUSNO
RW1
RXO
TCN
TCURE
TEORI
TJX
TLC
TR2
W8F
WH7
WOQ
X7H
YAYTL
YKOAZ
YXANX
ZKX
~91
AAYXX
AGORE
AJBYB
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7X8
ID FETCH-LOGICAL-c365t-9fcd750c1d1370e26b4f1063fb5c37d637e26df54f5ff719f7a39e3b060c77ea3
ISSN 0268-1161
1460-2350
IngestDate Sun Sep 28 08:53:52 EDT 2025
Thu Apr 03 07:04:40 EDT 2025
Tue Jul 01 00:55:05 EDT 2025
Thu Apr 24 22:57:29 EDT 2025
Wed Apr 02 07:03:34 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 2
Keywords allografting
minimal residual disease
ovarian metastasis
ovarian tissue cryopreservation
fertility preservation
borderline ovarian tumor
Language English
License The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c365t-9fcd750c1d1370e26b4f1063fb5c37d637e26df54f5ff719f7a39e3b060c77ea3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0003-1794-0368
OpenAccessLink https://academic.oup.com/humrep/article-pdf/33/2/212/23602284/dex352.pdf
PMID 29281007
PQID 1981052093
PQPubID 23479
PageCount 8
ParticipantIDs proquest_miscellaneous_1981052093
pubmed_primary_29281007
crossref_citationtrail_10_1093_humrep_dex352
crossref_primary_10_1093_humrep_dex352
oup_primary_10_1093_humrep_dex352
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20180201
2018-02-01
PublicationDateYYYYMMDD 2018-02-01
PublicationDate_xml – month: 02
  year: 2018
  text: 20180201
  day: 01
PublicationDecade 2010
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Human reproduction (Oxford)
PublicationTitleAlternate Hum Reprod
PublicationYear 2018
Publisher Oxford University Press
Publisher_xml – name: Oxford University Press
References Zanetta ( key 20180328162834_dex352C33) 2001; 19
Dolmans ( key 20180328162834_dex352C6) 2013; 99
Donnez ( key 20180328162834_dex352C11) 2013; 99
Gosden ( key 20180328162834_dex352C16) 1994; 9
Dolmans ( key 20180328162834_dex352C5) 2010; 116
Jensen ( key 20180328162834_dex352C18) 2017; 34
Dolmans ( key 20180328162834_dex352C7) 2016; 31
Fain-Kahn ( key 20180328162834_dex352C13) 2009; 24
Dittrich ( key 20180328162834_dex352C4) 2015; 103
Halimi ( key 20180328162834_dex352C17) 2013; 63
Lotz ( key 20180328162834_dex352C21) 2011; 95
Chen ( key 20180328162834_dex352C2) 2016; 9
Donnez ( key 20180328162834_dex352C10) 2013; 9
Donnez ( key 20180328162834_dex352C9) 2004; 364
Rosendahl ( key 20180328162834_dex352C26) 2010; 94
Luyckx ( key 20180328162834_dex352C22) 2013; 30
Donnez ( key 20180328162834_dex352C8) 2003; 79
Sayasneh ( key 20180328162834_dex352C28) 2011; 2011
Taylor ( key 20180328162834_dex352C30) 1929; 48
Morice ( key 20180328162834_dex352C24) 2006; 42
Uzan ( key 20180328162834_dex352C32) 2010; 21
Nisolle ( key 20180328162834_dex352C25) 2000; 74
Kim ( key 20180328162834_dex352C19) 2010; 57
Fortin ( key 20180328162834_dex352C15) 2007; 87
Tropé ( key 20180328162834_dex352C31) 2012; 26
Mangili ( key 20180328162834_dex352C23) 2016; 49
Buis ( key 20180328162834_dex352C1) 2013; 28
Donnez ( key 20180328162834_dex352C12) 2015; 32
Kristensen ( key 20180328162834_dex352C20) 2017; 107
Sanchez-Serrano ( key 20180328162834_dex352C27) 2009; 24
Daraï ( key 20180328162834_dex352C3) 2013; 19
Fauvet ( key 20180328162834_dex352C14) 2005; 83
Sumathi ( key 20180328162834_dex352C29) 2002; 55
References_xml – volume: 74
  start-page: 122
  year: 2000
  ident: key 20180328162834_dex352C25
  article-title: Histologic and ultrastructural evaluation of fresh and frozen-thawed human ovarian xenografts in nude mice
  publication-title: Fertil Steril
  doi: 10.1016/S0015-0282(00)00548-3
– volume: 9
  start-page: 735
  year: 2013
  ident: key 20180328162834_dex352C10
  article-title: Fertility preservation in women
  publication-title: Nat Rev Endocrinol
  doi: 10.1038/nrendo.2013.205
– volume: 9
  start-page: 16
  year: 2016
  ident: key 20180328162834_dex352C2
  article-title: Fertility-sparing surgery for young patients with borderline ovarian tumors (BOTs): single institution experience
  publication-title: J Ovarian Res
  doi: 10.1186/s13048-016-0226-y
– volume: 63
  start-page: 534
  year: 2013
  ident: key 20180328162834_dex352C17
  article-title: Claudin-18 overexpression in intestinal-type mucinous borderline tumour of the ovary
  publication-title: Histopathology
  doi: 10.1111/his.12182
– volume: 99
  start-page: 1503
  year: 2013
  ident: key 20180328162834_dex352C11
  article-title: Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2013.03.030
– volume: 116
  start-page: 2908
  year: 2010
  ident: key 20180328162834_dex352C5
  article-title: Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe
  publication-title: Blood
  doi: 10.1182/blood-2010-01-265751
– volume: 28
  start-page: 3358
  year: 2013
  ident: key 20180328162834_dex352C1
  article-title: Increased risk for ovarian cancer and borderline ovarian tumours in subfertile women with endometriosis
  publication-title: Human Reprod
  doi: 10.1093/humrep/det340
– volume: 94
  start-page: 2186
  year: 2010
  ident: key 20180328162834_dex352C26
  article-title: Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2009.11.032
– volume: 9
  start-page: 597
  year: 1994
  ident: key 20180328162834_dex352C16
  article-title: Restoration of fertility to oophorectomised sheep by ovarian autografts stored at −196°C
  publication-title: Human Reprod
  doi: 10.1093/oxfordjournals.humrep.a138556
– volume: 42
  start-page: 149
  year: 2006
  ident: key 20180328162834_dex352C24
  article-title: Borderline tumours of the ovary and fertility
  publication-title: Eur J Cancer
  doi: 10.1016/j.ejca.2005.07.029
– volume: 364
  start-page: 1405
  year: 2004
  ident: key 20180328162834_dex352C9
  article-title: Livebirth after orthotopic transplantation of cryopreserved ovarian tissue
  publication-title: Lancet
  doi: 10.1016/S0140-6736(04)17222-X
– volume: 103
  start-page: 462
  year: 2015
  ident: key 20180328162834_dex352C4
  article-title: Pregnancies and live births after 20 transplantations of cryopreserved ovarian tissue in a single center
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2014.10.045
– volume: 2011
  start-page: Article ID 140310
  year: 2011
  ident: key 20180328162834_dex352C28
  article-title: Endometriosis and ovarian cancer: a systematic review
  publication-title: ISRN Obstet Gynecol
  doi: 10.5402/2011/140310
– volume: 83
  start-page: 284
  year: 2005
  ident: key 20180328162834_dex352C14
  article-title: Fertility after conservative treatment for borderline ovarian tumors: a French multicenter study
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2004.10.009
– volume: 31
  start-page: 2292
  year: 2016
  ident: key 20180328162834_dex352C7
  article-title: Evaluation of minimal disseminated disease in cryopreserved ovarian tissue from bone and soft tissue sarcoma patients
  publication-title: Human Reprod
  doi: 10.1093/humrep/dew193
– volume: 57
  start-page: 587
  year: 2010
  ident: key 20180328162834_dex352C19
  article-title: Endocervical-like (Mullerian) mucinous borderline tumours of the ovary are frequently associated with the KRAS mutation
  publication-title: Histopathology
  doi: 10.1111/j.1365-2559.2010.03673.x
– volume: 34
  start-page: 325
  year: 2017
  ident: key 20180328162834_dex352C18
  article-title: 86 Successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children
  publication-title: J Assist Reprod Genet
  doi: 10.1007/s10815-016-0843-9
– volume: 99
  start-page: 1514
  year: 2013
  ident: key 20180328162834_dex352C6
  article-title: Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2013.03.027
– volume: 55
  start-page: 391
  year: 2002
  ident: key 20180328162834_dex352C29
  article-title: CD10 is useful in demonstrating endometrial stroma at ectopic sites and in confirming a diagnosis of endometriosis
  publication-title: J Clin Pathol
  doi: 10.1136/jcp.55.5.391
– volume: 95
  start-page: 2612
  year: 2011
  ident: key 20180328162834_dex352C21
  article-title: Xenotransplantation of cryopreserved ovarian tissue from patients with ovarian tumors into SCID mice—no evidence of malignant cell contamination
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2011.05.003
– volume: 19
  start-page: 151
  year: 2013
  ident: key 20180328162834_dex352C3
  article-title: Fertility and borderline ovarian tumor: a systematic review of conservative management, risk of recurrence and alternative options
  publication-title: Hum Reprod Update
  doi: 10.1093/humupd/dms047
– volume: 49
  start-page: 13
  year: 2016
  ident: key 20180328162834_dex352C23
  article-title: Fertility preservation in women with borderline ovarian tumours
  publication-title: Cancer Treat Rev
  doi: 10.1016/j.ctrv.2016.06.010
– volume: 24
  start-page: 2238
  year: 2009
  ident: key 20180328162834_dex352C27
  article-title: Malignant cells are not found in ovarian cortex from breast cancer patients undergoing ovarian cortex cryopreservation
  publication-title: Human Reprod
  doi: 10.1093/humrep/dep196
– volume: 87
  start-page: 591
  year: 2007
  ident: key 20180328162834_dex352C15
  article-title: Impact of infertility drugs after treatment of borderline ovarian tumors: results of a retrospective multicenter study
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2006.07.1503
– volume: 107
  start-page: 1206
  year: 2017
  ident: key 20180328162834_dex352C20
  article-title: Fertility preservation and refreezing of transplanted ovarian tissue—a potential new way of managing patients with low risk of malignant cell recurrence
  publication-title: Fertil Steril
  doi: 10.1016/j.fertnstert.2017.03.017
– volume: 32
  start-page: 1167
  year: 2015
  ident: key 20180328162834_dex352C12
  article-title: Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice
  publication-title: J Assist Reprod Genet
  doi: 10.1007/s10815-015-0544-9
– volume: 24
  start-page: 850
  year: 2009
  ident: key 20180328162834_dex352C13
  article-title: Feasibility of ovarian cryopreservation in borderline ovarian tumours
  publication-title: Human Reprod
  doi: 10.1093/humrep/den459
– volume: 30
  start-page: 1289
  year: 2013
  ident: key 20180328162834_dex352C22
  article-title: Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study
  publication-title: J Assist Reprod Genet
  doi: 10.1007/s10815-013-0065-3
– volume: 48
  start-page: 204
  year: 1929
  ident: key 20180328162834_dex352C30
  article-title: Malignant and semi-malignant tumours of the ovary
  publication-title: Surg Gynecol Obstet
– volume: 21
  start-page: 55
  year: 2010
  ident: key 20180328162834_dex352C32
  article-title: Outcomes after conservative treatment of advanced-stage serous borderline tumors of the ovary
  publication-title: Ann Oncol
  doi: 10.1093/annonc/mdp267
– volume: 19
  start-page: 2658
  year: 2001
  ident: key 20180328162834_dex352C33
  article-title: Behavior of borderline tumors with particular interest to persistence, recurrence, and progression to invasive carcinoma: a prospective study
  publication-title: J Clin Oncol
  doi: 10.1200/JCO.2001.19.10.2658
– volume: 26
  start-page: 325
  year: 2012
  ident: key 20180328162834_dex352C31
  article-title: Borderline ovarian tumours
  publication-title: Best Pract Res Clin Obstet Gynaecol
  doi: 10.1016/j.bpobgyn.2011.12.006
– volume: 79
  start-page: 1216
  year: 2003
  ident: key 20180328162834_dex352C8
  article-title: Safety of conservative management and fertility outcome in women with borderline tumors of the ovary
  publication-title: Fertil Steril
  doi: 10.1016/S0015-0282(03)00160-2
SSID ssj0016186
Score 2.4175017
Snippet Abstract STUDY QUESTION Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? SUMMARY ANSWER...
Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure? BOT cells were found in frozen-thawed...
Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure?STUDY QUESTIONIs transplantation of...
SourceID proquest
pubmed
crossref
oup
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 212
SubjectTerms Adult
Animals
Cryopreservation
Female
Fertility Preservation - adverse effects
Fertility Preservation - methods
Humans
Immunohistochemistry
Keratin-7 - genetics
Keratin-7 - metabolism
Mice
Mice, SCID
Mucin-1 - genetics
Mucin-1 - metabolism
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Ovarian Neoplasms - surgery
Ovary - metabolism
Ovary - pathology
Ovary - transplantation
Prospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Safety
Tissue Transplantation - adverse effects
Tissue Transplantation - methods
Transplantation, Heterologous
Title Safety of ovarian tissue transplantation in patients with borderline ovarian tumors
URI https://www.ncbi.nlm.nih.gov/pubmed/29281007
https://www.proquest.com/docview/1981052093
Volume 33
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAFT
  databaseName: Open Access Digital Library
  customDbUrl:
  eissn: 1460-2350
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0016186
  issn: 0268-1161
  databaseCode: KQ8
  dateStart: 19960101
  isFulltext: true
  titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html
  providerName: Colorado Alliance of Research Libraries
– providerCode: PRVBFR
  databaseName: Free Medical Journals
  customDbUrl:
  eissn: 1460-2350
  dateEnd: 20241001
  omitProxy: true
  ssIdentifier: ssj0016186
  issn: 0268-1161
  databaseCode: DIK
  dateStart: 19960101
  isFulltext: true
  titleUrlDefault: http://www.freemedicaljournals.com
  providerName: Flying Publisher
– providerCode: PRVFQY
  databaseName: GFMER Free Medical Journals
  customDbUrl:
  eissn: 1460-2350
  dateEnd: 20241001
  omitProxy: true
  ssIdentifier: ssj0016186
  issn: 0268-1161
  databaseCode: GX1
  dateStart: 19960101
  isFulltext: true
  titleUrlDefault: http://www.gfmer.ch/Medical_journals/Free_medical.php
  providerName: Geneva Foundation for Medical Education and Research
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9swEBdZB2MvY0v3kX2hwchT3NqSbcWPZV9lkDHWFPJmZFvqAo1dGqc0_UP29-6kk-2k7Vi3F2Mcn-L4Lnen-_gdIe-VX4SFkoEnwL4YUG3pZTKPPLBFGQtjHujchAYm3-LD4_DrLJr1er82qpZWdbaXX93aV_I_XIVrwFfTJfsPnG0XhQtwDvyFI3AYjnfi8ZHUCisqqgvY88Jftbbv0Qx-KJdnp7J0tYQdgKrrZsss5KZ1MVvS1aJyqR3nrGKA38BeWlRYFJQxthhuRBAmcmlCIycKszg_wOwqHGXkChGXpn8dc_tzed5-8NHU2FxhqW5uzFMrfPAgOOQZoQ9g8bmLubr4RDBuSppbNcZi2KcGCLm-p1DNhrHvMY6Qs40eRkAMJ29sS6myDfvsVOwN1Y-wWD9XC3gtcFKoSx6xzso1mf1rxq8tScRkPE9xgRTJ75H7TMSxmYzxZdaWDtkZAxi7w1_msFuBfB_J95F8y9fZ6p-8sY2x7sz0MXnk9iH0AIXqCempsk92D0pZV4s1HVJbGWxTLn3yYOIKMPpk-B2hztcjOu0695YjS9GCoK93yRHKJq00dQJGUTbpNdmk85I2skmNbNJONjtSK5tPyfHnT9MPh54b4OHlPI5qL9F5AR5pHhQBF75icRbqAHxinUU5F0XMBVwrdBTqSGsRJFpIniie-bGfC6Ekf0Z2yqpULwgVWaRZIUCx6BD8KSk5fIOOCwXbAX-cBAMyal51mjt0ezNk5TS9lbEDMmxvP0NYlz_d-A749td7Gq6moJxNxk2Wqlot0yAZB7bQjA_Ic2R3uxRL2NiUKL2866O8Ig-7_9drslOfr9Qb8Ijr7K0Vz9_dpb_Y
linkProvider Geneva Foundation for Medical Education and Research
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Safety+of+ovarian+tissue+transplantation+in+patients+with+borderline+ovarian+tumors&rft.jtitle=Human+reproduction+%28Oxford%29&rft.au=Masciangelo%2C+Rossella&rft.au=Bosisio%2C+Chiara&rft.au=Donnez%2C+Jacques&rft.au=Amorim%2C+Christiani+A&rft.date=2018-02-01&rft.issn=0268-1161&rft.eissn=1460-2350&rft.volume=33&rft.issue=2&rft.spage=212&rft.epage=219&rft_id=info:doi/10.1093%2Fhumrep%2Fdex352&rft.externalDBID=n%2Fa&rft.externalDocID=10_1093_humrep_dex352
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0268-1161&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0268-1161&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0268-1161&client=summon