Enantioselective determination of terazosin in human plasma by normal phase high-performance liquid chromatography–electrospray mass spectrometry
A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introducti...
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| Published in | Journal of Chromatography A Vol. 794; no. 1-2; pp. 45 - 56 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
23.01.1998
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0021-9673 |
| DOI | 10.1016/S0021-9673(97)00892-3 |
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| Abstract | A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introduction of the normal-phase eluent into an electrospray source followed by mass-selective detection. No pre-column derivatization was required prior to analysis. The chiral stationary phase used was Chiralpak AD 100 mm×2.1 mm I.D. (10 μm particle size). The method was utilized to determine the concentrations of terazosin enantiomers in human subjects following a 5 mg single oral dose. Results were compared with those from an enantioselective HPLC–fluorescence method from the same subject plasma samples. The LC–MS results confirm with confidence that the two terazosin enantiomers have different elimination profiles. |
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| AbstractList | A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introduction of the normal-phase eluent into an electrospray source followed by mass-selective detection. No pre-column derivatization was required prior to analysis. The chiral stationary phase used was Chiralpak AD 100 mm x 2.1 mm I.D. (10 microns particle size). The method was utilized to determine the concentrations of terazosin enantiomers in human subjects following a 5 mg single oral dose. Results were compared with those from an enantioselective HPLC-fluorescence method from the same subject plasma samples. The LC-MS results confirm with confidence that the two terazosin enantiomers have different elimination profiles.A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introduction of the normal-phase eluent into an electrospray source followed by mass-selective detection. No pre-column derivatization was required prior to analysis. The chiral stationary phase used was Chiralpak AD 100 mm x 2.1 mm I.D. (10 microns particle size). The method was utilized to determine the concentrations of terazosin enantiomers in human subjects following a 5 mg single oral dose. Results were compared with those from an enantioselective HPLC-fluorescence method from the same subject plasma samples. The LC-MS results confirm with confidence that the two terazosin enantiomers have different elimination profiles. A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introduction of the normal-phase eluent into an electrospray source followed by mass-selective detection. No pre-column derivatization was required prior to analysis. The chiral stationary phase used was Chiralpak AD 100 mm x 2.1 mm I.D. (10 microns particle size). The method was utilized to determine the concentrations of terazosin enantiomers in human subjects following a 5 mg single oral dose. Results were compared with those from an enantioselective HPLC-fluorescence method from the same subject plasma samples. The LC-MS results confirm with confidence that the two terazosin enantiomers have different elimination profiles. A sensitive and selective analytical method for the enantioselective determination of terazosin in human plasma has been developed. The chromatography is based on the normal-phase chiral separation utilizing the analogue prazosin as the internal standard. The detection involves the direct introduction of the normal-phase eluent into an electrospray source followed by mass-selective detection. No pre-column derivatization was required prior to analysis. The chiral stationary phase used was Chiralpak AD 100 mm×2.1 mm I.D. (10 μm particle size). The method was utilized to determine the concentrations of terazosin enantiomers in human subjects following a 5 mg single oral dose. Results were compared with those from an enantioselective HPLC–fluorescence method from the same subject plasma samples. The LC–MS results confirm with confidence that the two terazosin enantiomers have different elimination profiles. |
| Author | Zavitsanos, A.Paul Alebic-Kolbah, Tanja |
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| Cites_doi | 10.1016/S0021-9673(96)00756-X 10.1002/j.1552-4604.1993.tb01965.x 10.1016/S0378-4347(00)84711-8 |
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| Keywords | Terazosin Prazosin Enantiomer separation |
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| References | Patterson (BIB4) 1984; 311 Notices in Federal Register, 57, No. 102, May 27, 1992; also Announcement in Chirality, 4 (1992) 338. Alebic-Kolbah, Zavitsanos (BIB3) 1997; 759 Kyncl (BIB1) 1993; 33 Alebic-Kolbah (10.1016/S0021-9673(97)00892-3_BIB3) 1997; 759 10.1016/S0021-9673(97)00892-3_BIB2 Patterson (10.1016/S0021-9673(97)00892-3_BIB4) 1984; 311 Kyncl (10.1016/S0021-9673(97)00892-3_BIB1) 1993; 33 |
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| SubjectTerms | Adrenergic alpha-Antagonists - blood Chromatography, High Pressure Liquid Humans Indicators and Reagents Mass Spectrometry Prazosin Prazosin - analogs & derivatives Prazosin - blood Spectrometry, Fluorescence Stereoisomerism Terazosin |
| Title | Enantioselective determination of terazosin in human plasma by normal phase high-performance liquid chromatography–electrospray mass spectrometry |
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