The source of the nitrogen atom for the biosynthesis of anthranilic acid

Chorismic acid was converted into anthranilic acid using cell-free extracts of Aerobacter aerogenes and l-[ amide- 15N]glutamine as nitrogen source. Mass spectroscopy of the anthranilic acid showed that the amide-nitrogen of glutamine was incorporated. NH 4+ ions were also found effective as a nitro...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 93; no. 1; pp. 78 - 84
Main Authors Edwards, J.M., Gibson, F., Jackman, L.M., Shannon, J.S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 09.10.1964
Subjects
Ammonia
Chemical Phenomena
Chemistry
Enterobacter aerogenes
Glutamine
Leucine
Metabolism
Nitrogen
OldMedline
ortho-Aminobenzoates
Radioisotopes
AMMONIA
ANTHRANILIC ACID
GLUTAMINE
METABOLISM
AEROBACTER AEROGENES
EXPERIMENTAL LAB STUDY
CHEMISTRY
NITROGEN
RADIOISOTOPES
LEUCINE
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ISSN0304-4165
0006-3002
1872-8006
DOI10.1016/0304-4165(64)90262-4

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Summary:Chorismic acid was converted into anthranilic acid using cell-free extracts of Aerobacter aerogenes and l-[ amide- 15N]glutamine as nitrogen source. Mass spectroscopy of the anthranilic acid showed that the amide-nitrogen of glutamine was incorporated. NH 4+ ions were also found effective as a nitrogen source. Anthranilic acid synthesis with NH 4+, unlike that with glutamine, was not inhibited by 6-diazo-5-oxo- l-nor-leucine. End-product inhibition of the conversion of chorismic acid into anthranilic acid by l-tryptophan was effective with either nitrogen source.
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(64)90262-4