Human liver nuclear transcortin its postulated role in glucocorticoid regulation of genetic activity

• Published in: Biochimica et biophysica acta Vol. 362; no. 2; pp. 332 - 345
• Main Authors: Amaral, Leonard, Lin, Kobkul, Samuels, Arthur J., Werthamer, Seymour
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.09.1974
• Subjects: Animals; Autopsy; Binding Sites; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Centrifugation, Density Gradient; Chromatin - metabolism; Dialysis; DNA-Directed RNA Polymerases - metabolism; Drug Stability; Electrophoresis, Disc; Escherichia coli - enzymology; Fluoresceins; Humans; Hydrocortisone - metabolism; Immunodiffusion; Immunoelectrophoresis; Liver - cytology; Liver - metabolism; Liver - pathology; Mercaptoethanol - pharmacology; Mercuribenzoates - pharmacology; Mercury - pharmacology; Protein Binding; Rabbits - immunology; Receptors, Cell Surface - drug effects; RNA, Bacterial - biosynthesis; Temperature; Transcortin - metabolism; Transcortin - pharmacology; Transcription, Genetic; Tritium
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(74)90226-8

Highly purified human transcortin was injected into rabbits, and the antibody subsequently obtained was employed for the demonstration of transcortin-like molecules within various subcellular fractions of the human liver cell. Results obtained via quantitative double diffusion ouchterlony procedures indicate that proteins extracted from the nucleus or from chromatin form continuous precipitin lines of identity with those of transcortin. Fluorescein-tagged anti-transcortin permitted the visual localization of this molecule within isolated nuclei. Cortisol binding studies of all the subcellular fractions, particularly that extracted from the chromatin, suggest that such proteins do indeed bind cortisol specifically, as well as responding to exogeneous additions to the buffer (sulfhydryl reagents) as does purified transcortin. Purified transcortin when dialyzed exhaustively loses its cortisol binding ability, although the latter can be restored after its incubation with chromatin at 4°C. The restoration of such activity is dependent upon a dialyzable, heat-resistant chromatin component which itself lacks cortisol binding activity and which increases the sedimentation characteristics of dialyzed transcortin. The effect of transcortin on the in vitro synthesis of RNA in an Escherichia coli RNA polymerase human liver chromatin system is also presented. All of the above results are interpreted to indicate that transcortin is involved directly in the regulation of that genetic activity observed subsequent to the administration of cortisol.