Analysis of the Water Soluble Extract of Comedones

The water soluble fraction of 713 open comedones, pooled from both the face and back of 47 subjects representing all grades of acne, were analyzed for total protein content, carbohydrate content, and for identification of specific proteins. In the water soluble fraction, the protein content represen...

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Published inJournal of investigative dermatology Vol. 71; no. 5; pp. 330 - 333
Main Authors Hernandez, Leopoldo G, Puhvel, S Madli
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.1978
Subjects
Acne Vulgaris - enzymology
Acne Vulgaris - immunology
Acne Vulgaris - physiopathology
Adolescent
Adult
Antigens, Bacterial - analysis
Carbohydrates - analysis
Esterases - analysis
Female
Glycoproteins - analysis
Humans
Hyaluronoglucosaminidase - analysis
Male
Propionibacterium acnes - immunology
Proteins - analysis
Serum Albumin - analysis
Skin - analysis
Skin - enzymology
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ISSN0022-202X
1523-1747
DOI10.1111/1523-1747.ep12529831

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Abstract The water soluble fraction of 713 open comedones, pooled from both the face and back of 47 subjects representing all grades of acne, were analyzed for total protein content, carbohydrate content, and for identification of specific proteins. In the water soluble fraction, the protein content represented 11.5%, and carbohydrate content 0.2% of the total comedonal crude weight. Esterase and hyaluronidase activity was demonstrated. Propionibacterium acnes antigenic material, serum albumin, and serum Zn alpha 2 glycoprotein, a minor serum constituent, were identified by immunodiffusion and immunoelectrophoresis.
AbstractList The water soluble fraction of 713 open comedones, pooled from both the face and back of 47 subjects representing all grades of acne, were analyzed for total protein content, carbohydrate content, and for identification of specific proteins. In the water soluble fraction, the protein content represented 11.5%, and carbohydrate content 0.2% of the total comedonal crude weight. Esterase and hyaluronidase activity was demonstrated. Propionibacterium acnes antigenic material, serum albumin, and serum Zn alpha 2 glycoprotein, a minor serum constituent, were identified by immunodiffusion and immunoelectrophoresis.
The water soluble fraction of 713 open comedones, pooled from both the face and back of 47 subjects representing all grades of acne, were analyzed for total protein content, carbohydrate content, and for identification of specific proteins. In the water soluble fraction, the protein content represented 11.5%, and carbohydrate content 0.2% of the total comedonal crude weight. Esterase and hyaluronidase activity was demonstrated. Propionibacterium acnes antigenic material, serum albumin, and serum Zn alpha 2 glycoprotein, a minor serum constituent, were identified by immunodiffusion and immunoelectrophoresis.The water soluble fraction of 713 open comedones, pooled from both the face and back of 47 subjects representing all grades of acne, were analyzed for total protein content, carbohydrate content, and for identification of specific proteins. In the water soluble fraction, the protein content represented 11.5%, and carbohydrate content 0.2% of the total comedonal crude weight. Esterase and hyaluronidase activity was demonstrated. Propionibacterium acnes antigenic material, serum albumin, and serum Zn alpha 2 glycoprotein, a minor serum constituent, were identified by immunodiffusion and immunoelectrophoresis.
Author Hernandez, Leopoldo G
Puhvel, S Madli
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Cites_doi 10.1111/j.1365-2133.1977.tb15067.x
10.1001/archderm.1972.01620100033009
10.1038/jid.1959.139
10.2340/0001555557117120
10.1111/1523-1747.ep12260695
10.1016/S0021-9258(19)52451-6
10.1111/1523-1747.ep12724149
10.1111/1523-1747.ep12676801
10.1111/1523-1747.ep12551495
10.1038/jid.1966.151
10.1139/y59-099
10.1038/jid.1965.10
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References Puhvel, Reisner (bb0040) 1972; 106
Marples, Downing, Kligman (bb0085) 1971; 56
Fisher (bb0070) 1966; 47
Kochwa (bb0050) 1976
Puhvel, Amirian, Weintraub, Reisner (bb0060) 1977; 97
Fisher (bb0065) 1965; 44
Crowle (bb0045) 1961
Dische (bb0030) 1962
Marples, McGinley, Mills (bb0080) 1973; 60
Lowry, Rosebrough, Farr, Randall (bb0025) 1951; 193
Lees, Strauss, Downing, Pochi, Bachta (bb0015) 1977; 57
Kligman (bb0095) 1974; 62
Strauss, Mescon (bb0090) 1959; 33
Jamieson, Greenwalt (bb0055) 1971
Jamieson, Greenwalt (bb0075) 1971
Bligh, Over (bb0020) 1959; 37
Puhvel, Reisner (bb0035) 1972; 58
Puhvel, Sakamoto (bb0010) 1978
Kligman (10.1111/1523-1747.ep12529831_bb0095) 1974; 62
Puhvel (10.1111/1523-1747.ep12529831_bb0060) 1977; 97
Kochwa (10.1111/1523-1747.ep12529831_bb0050) 1976
Puhvel (10.1111/1523-1747.ep12529831_bb0010) 1978
Fisher (10.1111/1523-1747.ep12529831_bb0065) 1965; 44
Bligh (10.1111/1523-1747.ep12529831_bb0020) 1959; 37
Crowle (10.1111/1523-1747.ep12529831_bb0045) 1961
Fisher (10.1111/1523-1747.ep12529831_bb0070) 1966; 47
Puhvel (10.1111/1523-1747.ep12529831_bb0035) 1972; 58
Puhvel (10.1111/1523-1747.ep12529831_bb0040) 1972; 106
Jamieson (10.1111/1523-1747.ep12529831_bb0075) 1971
Marples (10.1111/1523-1747.ep12529831_bb0085) 1971; 56
Dische (10.1111/1523-1747.ep12529831_bb0030) 1962
Lowry (10.1111/1523-1747.ep12529831_bb0025) 1951; 193
Strauss (10.1111/1523-1747.ep12529831_bb0090) 1959; 33
Jamieson (10.1111/1523-1747.ep12529831_bb0055) 1971
Lees (10.1111/1523-1747.ep12529831_bb0015) 1977; 57
Marples (10.1111/1523-1747.ep12529831_bb0080) 1973; 60
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SubjectTerms Acne Vulgaris - enzymology
Acne Vulgaris - immunology
Acne Vulgaris - physiopathology
Adolescent
Adult
Antigens, Bacterial - analysis
Carbohydrates - analysis
Esterases - analysis
Female
Glycoproteins - analysis
Humans
Hyaluronoglucosaminidase - analysis
Male
Propionibacterium acnes - immunology
Proteins - analysis
Serum Albumin - analysis
Skin - analysis
Skin - enzymology
Title Analysis of the Water Soluble Extract of Comedones
URI https://dx.doi.org/10.1111/1523-1747.ep12529831
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