A fully automated nanoelectrospray tandem mass spectrometric method for analysis of Caco-2 samples
Caco‐2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem ma...
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| Published in | Rapid communications in mass spectrometry Vol. 17; no. 14; pp. 1573 - 1578 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Chichester, UK
John Wiley & Sons, Ltd
01.01.2003
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0951-4198 1097-0231 |
| DOI | 10.1002/rcm.1087 |
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| Abstract | Caco‐2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time‐consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI‐MS/MS), is introduced. This novel approach requires an off‐line ZipTip™ desalting step followed by automated nanoESI‐MS/MS, using the NanoMate™ 100 and ESI Chip™. In addition to reduced method development time, automated nanoESI‐MS/MS also offers no carry‐over between samples, low sample consumption, and ease‐of‐use as compared with conventional pulled‐capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high‐throughput. A comparison of Caco‐2 samples analyzed both by LC/MS/MS and by automated nanoESI‐MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI‐MS/MS were in excellent agreement. Copyright © 2003 John Wiley & Sons, Ltd. |
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| AbstractList | Caco‐2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time‐consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI‐MS/MS), is introduced. This novel approach requires an off‐line ZipTip™ desalting step followed by automated nanoESI‐MS/MS, using the NanoMate™ 100 and ESI Chip™. In addition to reduced method development time, automated nanoESI‐MS/MS also offers no carry‐over between samples, low sample consumption, and ease‐of‐use as compared with conventional pulled‐capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high‐throughput. A comparison of Caco‐2 samples analyzed both by LC/MS/MS and by automated nanoESI‐MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI‐MS/MS were in excellent agreement. Copyright © 2003 John Wiley & Sons, Ltd. Caco-2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time-consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI-MS/MS), is introduced. This novel approach requires an off-line ZipTip desalting step followed by automated nanoESI-MS/MS, using the NanoMate 100 and ESI Chip. In addition to reduced method development time, automated nanoESI-MS/MS also offers no carry-over between samples, low sample consumption, and ease-of-use as compared with conventional pulled-capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high-throughput. A comparison of Caco-2 samples analyzed both by LC/MS/MS and by automated nanoESI-MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI-MS/MS were in excellent agreement.Caco-2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time-consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI-MS/MS), is introduced. This novel approach requires an off-line ZipTip desalting step followed by automated nanoESI-MS/MS, using the NanoMate 100 and ESI Chip. In addition to reduced method development time, automated nanoESI-MS/MS also offers no carry-over between samples, low sample consumption, and ease-of-use as compared with conventional pulled-capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high-throughput. A comparison of Caco-2 samples analyzed both by LC/MS/MS and by automated nanoESI-MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI-MS/MS were in excellent agreement. Caco-2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time-consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI-MS/MS), is introduced. This novel approach requires an off-line ZipTip desalting step followed by automated nanoESI-MS/MS, using the NanoMate 100 and ESI Chip. In addition to reduced method development time, automated nanoESI-MS/MS also offers no carry-over between samples, low sample consumption, and ease-of-use as compared with conventional pulled-capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high-throughput. A comparison of Caco-2 samples analyzed both by LC/MS/MS and by automated nanoESI-MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI-MS/MS were in excellent agreement. |
| Author | Korfmacher, Walter A. Chu, Inhou Li, Cheng Van Pelt, Colleen K. Henion, Jack Liu, Tongtong Zhang, Sheng Fung, Eliza |
| Author_xml | – sequence: 1 givenname: Colleen K. surname: Van Pelt fullname: Van Pelt, Colleen K. email: cvanpelt@advion.com organization: Advion BioSciences, Inc., 15 Catherwood Road, Ithaca, NY 14850, USA – sequence: 2 givenname: Sheng surname: Zhang fullname: Zhang, Sheng organization: Advion BioSciences, Inc., 15 Catherwood Road, Ithaca, NY 14850, USA – sequence: 3 givenname: Eliza surname: Fung fullname: Fung, Eliza organization: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA – sequence: 4 givenname: Inhou surname: Chu fullname: Chu, Inhou organization: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA – sequence: 5 givenname: Tongtong surname: Liu fullname: Liu, Tongtong organization: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA – sequence: 6 givenname: Cheng surname: Li fullname: Li, Cheng organization: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA – sequence: 7 givenname: Walter A. surname: Korfmacher fullname: Korfmacher, Walter A. organization: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA – sequence: 8 givenname: Jack surname: Henion fullname: Henion, Jack organization: Advion BioSciences, Inc., 15 Catherwood Road, Ithaca, NY 14850, USA |
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| Cites_doi | 10.1093/jnci/59.1.221 10.1016/0006-291X(91)91647-U 10.1002/(SICI)1096-9888(200001)35:1<71::AID-JMS915>3.0.CO;2-5 10.1023/A:1011992518592 10.1021/ac000325y 10.1021/ac0260692 10.1016/S0016-5085(89)80072-1 10.1023/A:1012102522787 |
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| References | Hidalgo IJ, Raub TJ, Borchardt RT. Gastroenterology 1989; 96: 736. Artursson P, Karlsson J. Biochem. Biophys. Res. Commun. 1991; 175: 880. Van Pelt CK, Zhang S, Henion JD. J. Biomol. Technol. 2002; 13: 72. Jackson MJ. In Physiology of the Gastrointestinal Tract, Johnson LR (ed). Raven Press: New York, 1987; 1597-1621. Schultz GA, Corso TN, Prosser SJ, Zhang S. Anal. Chem. 2000; 72: 4058. Fogh J, Fogh JM, Orfeo T. J. Natl. Cancer Inst. 1977; 59: 221. Yee S. Pharm. Res. 1997; 14: 763. Polli JE, Ginski MJ. Pharm. Res. 1998; 15: 47. Dethy JM, Ackermann BL, Delatour C, Henion JD, Schultz GA. Anal. Chem. 2003; 75: 805. Artursson P. Crit. Rev. Ther. Drug Carrier Syst. 1991; 8: 305. Wang Z, Hop CE, Leung KH, Pang J. J. Mass Spectrom. 2000; 35: 71. 1987 2000; 72 1989; 96 1991; 175 1998; 15 1977; 59 2000; 35 1991; 8 2003; 75 1997; 14 2002; 13 Van Pelt CK (e_1_2_6_10_2) 2002; 13 e_1_2_6_7_2 e_1_2_6_9_2 e_1_2_6_4_2 e_1_2_6_3_2 e_1_2_6_6_2 e_1_2_6_5_2 Artursson P (e_1_2_6_8_2) 1991; 8 e_1_2_6_12_2 e_1_2_6_11_2 Jackson MJ (e_1_2_6_2_2) 1987 |
| References_xml | – reference: Dethy JM, Ackermann BL, Delatour C, Henion JD, Schultz GA. Anal. Chem. 2003; 75: 805. – reference: Wang Z, Hop CE, Leung KH, Pang J. J. Mass Spectrom. 2000; 35: 71. – reference: Artursson P. Crit. Rev. Ther. Drug Carrier Syst. 1991; 8: 305. – reference: Jackson MJ. In Physiology of the Gastrointestinal Tract, Johnson LR (ed). Raven Press: New York, 1987; 1597-1621. – reference: Schultz GA, Corso TN, Prosser SJ, Zhang S. Anal. Chem. 2000; 72: 4058. – reference: Fogh J, Fogh JM, Orfeo T. J. Natl. Cancer Inst. 1977; 59: 221. – reference: Artursson P, Karlsson J. Biochem. Biophys. Res. Commun. 1991; 175: 880. – reference: Van Pelt CK, Zhang S, Henion JD. J. Biomol. Technol. 2002; 13: 72. – reference: Polli JE, Ginski MJ. Pharm. Res. 1998; 15: 47. – reference: Hidalgo IJ, Raub TJ, Borchardt RT. Gastroenterology 1989; 96: 736. – reference: Yee S. Pharm. Res. 1997; 14: 763. – volume: 13 start-page: 72 year: 2002 publication-title: J. Biomol. Technol. – volume: 59 start-page: 221 year: 1977 publication-title: J. Natl. Cancer Inst. – volume: 75 start-page: 805 year: 2003 publication-title: Anal. Chem. – start-page: 1597 year: 1987 end-page: 1621 – volume: 14 start-page: 763 year: 1997 publication-title: Pharm. Res. – volume: 15 start-page: 47 year: 1998 publication-title: Pharm. Res. – volume: 35 start-page: 71 year: 2000 publication-title: J. Mass Spectrom. – volume: 96 start-page: 736 year: 1989 publication-title: Gastroenterology – volume: 175 start-page: 880 year: 1991 publication-title: Biochem. Biophys. Res. Commun. – volume: 8 start-page: 305 year: 1991 publication-title: Crit. Rev. Ther. Drug Carrier Syst. – volume: 72 start-page: 4058 year: 2000 publication-title: Anal. Chem. – ident: e_1_2_6_3_2 doi: 10.1093/jnci/59.1.221 – volume: 13 start-page: 72 year: 2002 ident: e_1_2_6_10_2 publication-title: J. Biomol. Technol. – ident: e_1_2_6_5_2 doi: 10.1016/0006-291X(91)91647-U – ident: e_1_2_6_9_2 doi: 10.1002/(SICI)1096-9888(200001)35:1<71::AID-JMS915>3.0.CO;2-5 – ident: e_1_2_6_7_2 doi: 10.1023/A:1011992518592 – ident: e_1_2_6_11_2 doi: 10.1021/ac000325y – volume: 8 start-page: 305 year: 1991 ident: e_1_2_6_8_2 publication-title: Crit. Rev. Ther. Drug Carrier Syst. – ident: e_1_2_6_12_2 doi: 10.1021/ac0260692 – start-page: 1597 volume-title: Physiology of the Gastrointestinal Tract year: 1987 ident: e_1_2_6_2_2 – ident: e_1_2_6_4_2 doi: 10.1016/S0016-5085(89)80072-1 – ident: e_1_2_6_6_2 doi: 10.1023/A:1012102522787 |
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| SubjectTerms | Algorithms Autoanalysis Caco-2 Cells - chemistry Calibration Cell Membrane Permeability Chromatography, High Pressure Liquid Chromatography, Liquid Drug Evaluation, Preclinical Humans Nanotechnology Robotics Spectrometry, Mass, Electrospray Ionization |
| Title | A fully automated nanoelectrospray tandem mass spectrometric method for analysis of Caco-2 samples |
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