Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar

• Published in: Forestry Studies in China Vol. 14; no. 1; pp. 1 - 7
• Main Authors: Song, Yue-peng, Jiang, Xi-bing, Zhang, Man, Wang, Ze-liang, Bo, Wen-hao, An, Xin-min, Zhang, De-qiang, Zhang, Zhi-yi
• Format: Journal Article
• Language: English
• Published: Heidelberg Beijing Forestry University 01.03.2012; Springer Nature B.V; National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, P.R.China%College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100, P.R.China
• Subjects: Biomedical and Life Sciences; China; Cluster analysis; Forestry; Forestry Management; Genetics; Life Sciences; Markers; Plant Ecology; Plant Genetics and Genomics; Polymorphism; Poplar; Research Article; SSR标记; Tree Biology; Wood Science & Technology; 基因型鉴定; 基因组; 多态性检测; 平均等位基因数; 杨树无性系; 评估; 遗传多样性; genomic-SSR; poplar; genetic differences; EST-SSR
• ISSN: 1008-1321; 2095-4034; 1993-0372; 2197-5620
• DOI: 10.1007/s11632-012-0106-5

We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show that the average number of alleles detected by genomic-SSR was 4.1, Shannon＇s index 1.0646, observed heterozygos- ity 0.4427 and expected heterozygosity 0.5523, while for the EST-SSR, the average number of alleles was 2.8, Shannon＇s index 0.6985, observed heterozygosity 0.2330 and expected heterozygosity 0.4684. Cluster analysis indicated that the EST-SSR capacity of genotypic identification was more precise than that of genomic-SSR. These resuks reveal that EST-SSR and genomic-SSR have statistically significant genetic differences in polymorphism detection and genotypic identification. These differences could provide a theoretical basis for the rational use of SSR markers in species diversity and other related research.