Ethylacetate extracts of the muscles of Anguilla japonica suppress glucose levels in db/db mice via activation of AMP-activated protein kinase

• Published in: Food science and biotechnology Vol. 23; no. 5; pp. 1695 - 1701
• Main Authors: Jeong, Yong-Tae, Yang, Ju Hye, Li, Xian, Kim, Geum Jin, Kim, Dong Soo, Kim, Cheorl-Ho, Na, Min Kyun, Chang, Hyeun Wook
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.10.2014; 한국식품과학회
• Subjects: Anguilla japonica; Chemistry; Chemistry and Materials Science; Food Science; Nutrition; Research Article; 식품과학; mouse; glucose uptake; AMP-activated protein kinase; L6 myotube; /
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-014-0231-0

Hypoglycemic effects of ethylacetate extracts of Anguilla japonica (EMA) muscles in db / db mice were investigated. To understand the mechanism responsible for the hypoglycemic effects of EMA, the effects of EMA on AMP-activated protein kinase (AMPK) activation in L6 myotubes and in vivo using type II diabetic db / db mice were analyzed. In L6 myotubes, the phosphorylation degrees of AMPK and acetyl-CoA carboxylase (ACC) were markedly increased and glucose uptake was significantly ( p <0.001) increased by EMA, compared with untretaed L6 myotubes. However, in L6 myotubes, these effects were abolished by compound C, an AMPK inhibitor. Moreover, EMA significantly reduced non-fasting blood glucose and serum insulin levels, and strongly induced AMPK phosphorylation in skeletal muscle tissues of db / db mice. EMA regulates glucose levels in L6 myotubes and in diabetic mice by activation of AMPK. Beneficial effects for diabetes treatment are indicated.