Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer

• Published in: Skin research and technology Vol. 25; no. 4; pp. 424 - 433
• Main Authors: Ma, Jie, Xu, Shuangjun, Wang, Xiaomeng, Zhang, Jing, Wang, Yaochi, Liu, Mengting, Jin, Ling, Wu, Mingshun, Qian, Danfeng, Li, Xueying, Zhen, Qi, Guo, Huimin, Gao, Jinping, Yang, Sen, Zhang, Xuejun
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.07.2019
• Subjects: Asian Continental Ancestry Group - ethnology; Epidermis - metabolism; Forearm; Healthy Volunteers; Humans; Intermediate Filament Proteins - metabolism; Keratin; Liquid chromatography; mass spectrometer; Mass spectrometry; Mass Spectrometry - instrumentation; Mass spectroscopy; Metabolism; natural moisturizing factor; Peptides; protein extraction; Proteins; Proteins - metabolism; Proteomes; Proteomics - methods; Skin; Skin - metabolism; skin barrier; skin protein; Software; stratum corneum; Trypsin; skin barrier; stratum corneum; protein extraction; skin protein; natural moisturizing factor; mass spectrometer
• ISSN: 0909-752X; 1600-0846; 1600-0846
• DOI: 10.1111/srt.12668

Objective The objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label‐free nanoflow liquid chromatography‐mass spectrometry (nLC‐MS). Materials and Methods Five consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin‐digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data‐dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2). Results In this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier. Conclusion This study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease.