Cooperativity in the annealing of DNA origamis

DNA based nanostructures built on a long single stranded DNA scaffold, known as DNA origamis, offer the possibility to organize various molecules at the nanometer scale in one pot experiments. The folding of the scaffold is guaranteed by the presence of short, single stranded DNA sequences (staples)...

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Bibliographic Details
Published inThe Journal of chemical physics Vol. 138; no. 1; p. 015105
Main Authors Arbona, Jean-Michel, Aimé, Jean-Pierre, Elezgaray, Juan
Format Journal Article
LanguageEnglish
Published United States 07.01.2013
Subjects
DNA - chemistry
Microscopy, Atomic Force
Models, Molecular
Nucleic Acid Conformation
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ISSN0021-9606
1089-7690
1089-7690
DOI10.1063/1.4773405

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Summary:DNA based nanostructures built on a long single stranded DNA scaffold, known as DNA origamis, offer the possibility to organize various molecules at the nanometer scale in one pot experiments. The folding of the scaffold is guaranteed by the presence of short, single stranded DNA sequences (staples), that hold together separate regions of the scaffold. In this paper, we modelize the annealing-melting properties of these DNA constructions. The model captures important features such as the hysteresis between melting and annealing, as well as the dependence upon the topology of the scaffold. We show that cooperativity between staples is critical to quantitatively explain the folding process of DNA origamis.
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ISSN:0021-9606
1089-7690
1089-7690
DOI:10.1063/1.4773405