Effects of waterborne copper on oxidative stress and immune responses in red seabream, Pagrus major

• Published in: Molecular & cellular toxicology Vol. 14; no. 3; pp. 291 - 301
• Main Authors: Choi, Cheol Young, Choe, Jong Ryeol, Shin, Yoon Sub, Kim, Tae Hwan, Choi, Ji Yong, Kim, Bong-Seok
• Format: Journal Article
• Language: English
• Published: Incheon The Korean Society of Toxicogenomics and Toxicoproteomics 01.07.2018; Springer Nature B.V; 대한독성 유전단백체 학회
• Subjects: Antioxidants; Biomedical and Life Sciences; Catalase; Cell Biology; Copper; Enzymes; Hydrogen peroxide; Immune response; Immunoglobulin M; Life Sciences; Lysozyme; Melatonin; mRNA; Original Paper; Oxidation; Oxidative stress; Pagrus major; Pharmacology/Toxicology; Superoxide dismutase; 생물학; Oxidative stress; Immune response; Copper; Red seabream; Antioxidant activity
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-018-0032-2

Backgrounds Copper (Cu) plays an essential role in many enzymatic processes including cellular respiration. In this study, we investigated the oxidative stress and immunity of red seabream, Pagrus major , exposed to different concentrations of Cu 2+ (10, 20, 30, and 40 μg/L) for 120 h. Methods We measured the expressions of mRNA, activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), the levels of plasma hydrogen peroxide (H 2 O 2 ) and lipid peroxide (LPO), and immune parameters lysozyme, immunoglobulin M (IgM), and melatonin. Results The mRNA expressions, activities of antioxidant enzymes, and the levels of plasma H 2 O 2 and LPO were significantly higher after exposure to 30 and 40 μg/L of Cu 2+ than after exposure to lower concentrations (0, 10, and 20 μg/L). However, the levels of plasma lysozyme, IgM, and melatonin were significantly lower after exposure to 30 and 40 μg/L of Cu 2+ . Conclusion These results indicate that Cu 2+ concentrations of 30 and 40 μg/L can induce an acute toxic oxidative stress and decrease the immune response in red seabream in aquarium conditions.