Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing
A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a gravity-induced flow injection chip（gFIC） was exploited to replace a pump.Also two charge coupled devices（CCDs） were used to detect HepG2 cells by...
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| Published in | Chemical research in Chinese universities Vol. 30; no. 2; pp. 211 - 215 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Heidelberg
Jilin University and The Editorial Department of Chemical Research in Chinese Universities
01.04.2014
Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P.R.China%Department of Information Science & Electronic Engineering, Zhejiang University,Hangzhou 310027, P.R.China |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1005-9040 2210-3171 |
| DOI | 10.1007/s40242-014-3415-5 |
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| Abstract | A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a gravity-induced flow injection chip（gFIC） was exploited to replace a pump.Also two charge coupled devices（CCDs） were used to detect HepG2 cells by SPRi and image processing,respectively.The results of two CCDs are associated.Protein A was used to modify the sensing surface.The inlet angle was carefully adjusted for the device to get an enhanced image.In the test,the contrast among cell solutions at different concentrations can be easily distinguished.The other CCD using image processing can tell false-positive in some degree.This detection is label-free,real time,and precise. |
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| AbstractList | A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a gravity-induced flow injection chip（gFIC） was exploited to replace a pump.Also two charge coupled devices（CCDs） were used to detect HepG2 cells by SPRi and image processing,respectively.The results of two CCDs are associated.Protein A was used to modify the sensing surface.The inlet angle was carefully adjusted for the device to get an enhanced image.In the test,the contrast among cell solutions at different concentrations can be easily distinguished.The other CCD using image processing can tell false-positive in some degree.This detection is label-free,real time,and precise. A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing. In this device, a gravity-induced flow injection chip(gFIC) was exploited to replace a pump. Also two charge coupled devices(CCDs) were used to detect HepG2 cells by SPRi and image processing, respectively. The results of two CCDs are associated. Protein A was used to modify the sensing surface. The inlet angle was carefully adjusted for the device to get an enhanced image. In the test, the contrast among cell solutions at different concentrations can be easily distinguished. The other CCD using image processing can tell false-positive in some degree. This detection is label-free, real time, and precise. |
| Author | YANG Mengchao JIN Xinyu YUAN Maokai LIU Fan LANG Yicong WU Zhongyu XIANG Liancheng ZHOU Chao OUYANG Bo JIN Wei MU Ying |
| AuthorAffiliation | Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P.R.China Department of Information Science ＆ Electronic Engineering, Zhejiang University,Hangzhou 310027, P.R.China |
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| Keywords | Gravity induced Surface plasmon resonance Image processing Tumor detection |
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| Notes | Surface plasmon resonance; Image processing; Tumor detection; Gravity induced A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a gravity-induced flow injection chip（gFIC） was exploited to replace a pump.Also two charge coupled devices（CCDs） were used to detect HepG2 cells by SPRi and image processing,respectively.The results of two CCDs are associated.Protein A was used to modify the sensing surface.The inlet angle was carefully adjusted for the device to get an enhanced image.In the test,the contrast among cell solutions at different concentrations can be easily distinguished.The other CCD using image processing can tell false-positive in some degree.This detection is label-free,real time,and precise. 22-1183/06 |
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| Publisher | Jilin University and The Editorial Department of Chemical Research in Chinese Universities Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P.R.China%Department of Information Science & Electronic Engineering, Zhejiang University,Hangzhou 310027, P.R.China |
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| Snippet | A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging（SPRi） and image processing.In this device,a... A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing. In this device, a... |
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| SubjectTerms | Analytical Chemistry CCD Chemistry Chemistry and Materials Science Chemistry/Food Science HepG2细胞 Inorganic Chemistry Organic Chemistry Physical Chemistry 图像处理 成像 检测装置 电荷耦合器件 肿瘤细胞 表面等离子体共振 |
| Title | Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing |
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