Influence of deep-eutectic and organic solvents on the recovery, molecular mass, and functional properties of dextran: Application using dextran film

The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtai...

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Published inInternational journal of biological macromolecules Vol. 293; p. 139202
Main Authors Rahman, Sameeha Syed Abdul, Pasupathi, Saroja, Karuppiah, Sugumaran
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.2025
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Online AccessGet full text
ISSN0141-8130
1879-0003
1879-0003
DOI10.1016/j.ijbiomac.2024.139202

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Abstract The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation. [Display omitted]
AbstractList The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation.
The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation.
The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation.The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation.
The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant activity, poly-dispersity index, and functional properties of microbial dextran. The optimized conditions for maximum dextran recovery were obtained using organic solvent found to be: supernatant: organic solvent - 1:4 v/v; organic solvents: ethanol, isopropanol, and acetone; temperature: 0 °C; and time: 16 h. Though a similar structure was obtained for dextran recovered using various solvents, the degree of branching varied, with DES-precipitated dextran having the highest branching of 20 % α-(1,3) linkages. Similarly, the molecular mass and functional properties of dextran were significantly influenced by solvents, enabling their different applications. Ethanol-recovered dextran had the highest water-holding capacity (441.7 ± 2.5 %), whose films could be used in agriculture to retain soil water. On the contrary, acetone-precipitated dextran had a maximum solubility (96.0 ± 0.4 %) and antioxidant activity, which could be used as food additives and/or wound dressings. The DES-recovered dextran exhibited high fat-binding capacity (287.1 ± 2.0 %) and emulsifying activity (50.0 ± 2.9 %), making it suitable as an emulsifier in mayonnaise, etc. The isopropanol-recovered dextran showed the highest mass-average molecular mass (3224.8 kDa) and could be employed as flocculants for waste-water remediation. [Display omitted]
ArticleNumber 139202
Author Rahman, Sameeha Syed Abdul
Karuppiah, Sugumaran
Pasupathi, Saroja
Author_xml – sequence: 1
  givenname: Sameeha Syed Abdul
  surname: Rahman
  fullname: Rahman, Sameeha Syed Abdul
  organization: Bioprocess Engineering Laboratory, School of Chemical and Biotechnology, SASTRA Deemed to be University, India
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  givenname: Saroja
  surname: Pasupathi
  fullname: Pasupathi, Saroja
  organization: Bioprocess Engineering Laboratory, School of Chemical and Biotechnology, SASTRA Deemed to be University, India
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  givenname: Sugumaran
  surname: Karuppiah
  fullname: Karuppiah, Sugumaran
  email: sugumaran@biotech.sastra.edu
  organization: Bioprocess Engineering Laboratory, School of Chemical and Biotechnology, Centre for Bioenergy, SASTRA Deemed to be University, India
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Keywords Dextran
Functional properties
Molasses
Deep eutectic solvents
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  ident: 10.1016/j.ijbiomac.2024.139202_bb0320
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  publication-title: Carbohydr. Polym.
  doi: 10.1016/j.carbpol.2015.09.035
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Snippet The novelty of this study is to examine the impact of different solvent systems, namely organic and deep eutectic solvents, on recovery yield, antioxidant...
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SubjectTerms acetone
Acetone - chemistry
antioxidant activity
Antioxidants - chemistry
Antioxidants - pharmacology
Deep eutectic solvents
Deep Eutectic Solvents - chemistry
Dextran
Dextrans - chemistry
Dextrans - isolation & purification
Dextrans - pharmacology
emulsifiers
ethanol
flocculants
Functional properties
isopropyl alcohol
mayonnaise
Molasses
Molecular Weight
remediation
soil water
Solubility
solvents
Solvents - chemistry
Temperature
wastewater
Water - chemistry
water holding capacity
Title Influence of deep-eutectic and organic solvents on the recovery, molecular mass, and functional properties of dextran: Application using dextran film
URI https://dx.doi.org/10.1016/j.ijbiomac.2024.139202
https://www.ncbi.nlm.nih.gov/pubmed/39733893
https://www.proquest.com/docview/3150138164
https://www.proquest.com/docview/3200328976
Volume 293
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