The Platelet Aggregation Score and Its Correlation to β- Thromboglobulin and Platelet Factor 4 Levels

• Published in: Clinical and applied thrombosis/hemostasis Vol. 1; no. 2; pp. 135 - 141
• Main Authors: Kaplan, Svetlana, Kaplan, Alexander, Marcoe, Karen F., Gey, Gerge O., Schiller, Harvey, Roby, Paul V., Fisher, Llod D., El-Massry, Sherif, Sauvage, Lester R.
• Format: Journal Article
• Language: English
• Published: Thousand Oaks, CA SAGE Publications 01.03.1995; SAGE PUBLICATIONS, INC
• ISSN: 1076-0296; 1938-2723
• DOI: 10.1177/107602969500100206

We describe a new method of interpreting platelet aggregometry results and its correlation to platelet secretory protein levels. We studied 123 healthy male volunteers, 37-85 years of age. The aggregation response to 2.5 μM ADP was measured in a standardized platelet suspension (SPS) prepared from platelet-rich plasma. Turbidimetic measurements included amplitude of the primary aggregation wave (Amp1), maximum aggregation amplitude (Amax), and area under the aggregation curve (Sagg). A platelet aggregation (PA) score was calculated as (Amp 1 /A max) ·Sagg ·(whole-blood platelet count/SPS platelet count). β-Thromboglobulin (β-TG)and . platelet factor 4 (PF4) levels were measured by radioimmunoassay. The PA score correlated significantly (p < 0.0001) with β-TG (r = 0.86) and PF4 (r = 0.87). It was concluded that the PA score correlates closely with individual subjects' propensities to platelet activation, as reflected by plasma β-TG and PF4 levels. Key Words: Platelets-Platelet aggregometry-Platelet secretory proteins.