Antibacterial and antibiofilm activities of linalool nanoemulsions against Salmonella Typhimurium

• Published in: Food bioscience Vol. 28; pp. 57 - 65
• Main Authors: Prakash, Anand, Vadivel, Vellingiri, Rubini, Durairajan, Nithyanand, Paramasivam
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.04.2019
• Subjects: antibacterial properties; Antibiofilm; biofilm; cell membranes; cinnamon; Coriandrum sativum; droplets; emulsifying; encapsulation; essential oils; food industry; Linalool; Nanoemulsions; pineapples; polysorbates; Salmonella Typhimurium; sensory evaluation; ultrasonics; water solubility; Nanoemulsions; Antibiofilm; Linalool; Salmonella Typhimurium
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2019.01.018

Linalool is the active constituent of essential oils of several plant species like coriander, rosewood and cinnamon, which is known to have strong antibacterial activity. However, its application in food systems is limited because of low water solubility, high volatility and low stability. The present study was undertaken to overcome such problems by encapsulating the linalool into nanoemulsions using Tween 80 and water using ultrasonic assisted emulsification. The stable nanoemulsions with a mean droplet diameter of 10.9 ± 0.1 nm was obtained at a 1:3 (v/v) ratio of linalool and Tween 80. The antibacterial and antibiofilm activities of the stable nanoemulsions were further investigated. The linalool nanoemulsions showed twofold enhanced antibacterial activity against Salmonella Typhimurium through their increased ability to disrupt cell membrane integrity. Moreover, compared to the pure linalool, the linalool nanoemulsions showed an 11.5% higher antibiofilm activity. Nanoemulsions also effectively reduced the S. Typhimurium biofilm on the surface of cut pineapple. Sensory analysis showed acceptance of fresh cut pineapple with linalool nanoemulsions treatment. Therefore, results of the present study indicated the potential use of linalool nanoemulsions as a natural antibacterial and antibiofilm agent against S. Typhimurium, which could find applications in the food industry.