Statistical Optimization of Process Parameters and Purification of Uricase Using Isolate Pseudomonas mosselii DSS002

• Published in: Indian journal of microbiology Vol. 65; no. 2; pp. 1089 - 1104
• Main Authors: Dudala, Sai Sushma, Venkateswarulu, T. C., Venkata Narayana, A., John Babu, D.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.06.2025; Springer Nature B.V
• Subjects: Algorithms; Artificial neural networks; Back propagation; Biomedical and Life Sciences; Enzymes; Genetic algorithms; Inoculum; Life Sciences; Medical Microbiology; Medical treatment; Microbiology; Molecular weight; Mutation; Neural networks; Neurons; Optimization; Optimization techniques; Original Research Article; pH effects; Pharmaceutical industry; Pharmaceuticals; Process parameters; Pseudomonas; Pseudomonas mosselii; Response surface methodology; Rheumatism; Statistical methods; Statistics; Temperature tolerance; Urate oxidase; Uric acid; Variance analysis; Artificial neural network; Uric acid; Box-Behnken design; Uricase; Genetic algorithm; Pseudomonas
• ISSN: 0046-8991; 0973-7715
• DOI: 10.1007/s12088-025-01467-y

Uricase holds significant pharmaceutical applications, particularly in treating diseases associated with elevated uric acid levels and serving as a diagnostic enzyme to detect uric acid in biological fluids. Enhancing uricase production is crucial to meet the demands of large-scale applications. This study focuses on optimizing process parameters for uricase production using advanced statistical methods, namely Response Surface Methodology (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA). Seven key process parameters were investigated: temperature, pH, medium volume, incubation time, inoculum size, inoculum age, and rpm. Conformational experimental studies at the ANN-GA predicted optimal conditions revealed a significant uricase activity of 63.92 ± 0.06 U/mL. The purified uricase exhibited specific activity of 92.48 U/mg and a molecular weight of approximately 32 kDa. Itdemonstrated remarkable stability, withstanding a wide pH range (6.0 to 10.0) and temperatures up to 50 °C, with an optimum pH of 9.0 and temperature of 30 °C. This broad pH and temperature tolerance of the purified uricase from Pseudomonas mosselii DSS002 underscores its potential as a valuable source for industrial-scale production, catering to various pharmaceutical applications. This study’s findings pave the way for efficient and scalable uricase production, offering promising implications for the pharmaceutical industry.