Photocurable O-carboxymethyl chitosan derivatives for biomedical applications: Synthesis, in vitro biocompatibility, and their wound healing effects

• Published in: Macromolecular research Vol. 20; no. 11; pp. 1144 - 1149
• Main Authors: Na, Ha-Na, Park, Shin-Hye, Kim, Kwang-Il, Kim, Mi Kyung, Son, Tae-Il
• Format: Journal Article
• Language: English
• Published: Heidelberg The Polymer Society of Korea 01.11.2012; 한국고분자학회
• Subjects: Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Nanochemistry; Nanotechnology; Physical Chemistry; Polymer Sciences; Soft and Granular Matter; 고분자공학; photo-curing; carboxymethyl chitosan; furfuryl glycidyl ether; biomedical materials
• ISSN: 1598-5032; 2092-7673
• DOI: 10.1007/s13233-012-0167-2

In this work, photocurable water-soluble chitosan derivatives were prepared for biomedical applications by modifying water-soluble O -carboxymethyl chitosan ( O -CMC) derivatives with furfuryl glycidyl ether ( O -CMC/FGE). Successful derivatization of chitosan to the final product ( O -CMC/FGE) was verified by UV and 1 H NMR spectral analysis. The degree of photo-crosslinking was measured by a flow distance experiment after exposure to visible light for a certain period of time. The degree of crosslinking increased linearly in proportion to exposure time. The in vitro cell viability test revealed a lack of cytotoxicity of O -CMC/FGE against mouse 3T3 fibroblasts. However, poor cell attachment was observed for the cells seeded onto the photocured O -CMC/FGE; this is likely due to the anionic nature of this material. O -CMC/FGE displayed wound healing effects in an in vivo animal experiment using a burn wound model. Due to its good biocompatibility, wound healing effect, and mild cross-linking condition, together with an inhibitory effect on cell attachment, O -CMC/FGE would be a promising candidate as an anti-adhesion material for biomedical applications.